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Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21.
J Biomed Nanotechnol 2012; 8(6):1000-5JB

Abstract

In this article, we present an easy route to prepare monodisperse core-shell Fe3O4@SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core-shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4@SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M(s)) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core-shell Fe3O4@SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars.

Authors+Show Affiliations

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing 210096, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23030008

Citation

Ma, Chao, et al. "Preparation and Characterization of Monodisperse Core-shell Fe3O4@SiO2 Microspheres and Its Application for Magnetic Separation of Nucleic Acids From E. Coli BL21." Journal of Biomedical Nanotechnology, vol. 8, no. 6, 2012, pp. 1000-5.
Ma C, Li C, He N, et al. Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21. J Biomed Nanotechnol. 2012;8(6):1000-5.
Ma, C., Li, C., He, N., Wang, F., Ma, N., Zhang, L., ... Wang, Z. (2012). Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21. Journal of Biomedical Nanotechnology, 8(6), pp. 1000-5.
Ma C, et al. Preparation and Characterization of Monodisperse Core-shell Fe3O4@SiO2 Microspheres and Its Application for Magnetic Separation of Nucleic Acids From E. Coli BL21. J Biomed Nanotechnol. 2012;8(6):1000-5. PubMed PMID: 23030008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21. AU - Ma,Chao, AU - Li,Chuanyan, AU - He,Nongyue, AU - Wang,Fang, AU - Ma,Ningning, AU - Zhang,Liming, AU - Lu,Zhuoxuan, AU - Ali,Zeeshan, AU - Xi,Zhijiang, AU - Li,Xiaolong, AU - Liang,Gaofeng, AU - Liu,Hongna, AU - Deng,Yan, AU - Xu,Lijian, AU - Wang,Zhifei, PY - 2012/10/4/entrez PY - 2012/10/4/pubmed PY - 2012/11/14/medline SP - 1000 EP - 5 JF - Journal of biomedical nanotechnology JO - J Biomed Nanotechnol VL - 8 IS - 6 N2 - In this article, we present an easy route to prepare monodisperse core-shell Fe3O4@SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core-shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4@SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M(s)) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core-shell Fe3O4@SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars. SN - 1550-7033 UR - https://www.unboundmedicine.com/medline/citation/23030008/Preparation_and_characterization_of_monodisperse_core_shell_Fe3O4@SiO2_microspheres_and_its_application_for_magnetic_separation_of_nucleic_acids_from_E__coli_BL21_ DB - PRIME DP - Unbound Medicine ER -