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Expression of NOX5 in human teratozoospermia compared to normozoospermia.
Andrologia 2013; 45(5):351-6A

Abstract

Spermatozoa are capable of producing small amounts of reactive oxygen species (ROS), and sperm in teratozoospermia generate more ROS than sperm in normozoospermia. The source of ROS production in ejaculated human sperm has not been fully clarified. Recently, NADPH oxidase 5 (NOX5) was detected in human sperm, and ROS generation by this enzyme was reported. We investigated the magnitude of NOX5 expression in normozoospermic (n = 12) and teratozoospermic (n = 13) semen samples with different percentages of abnormal sperm. The existence of NOX5 enzymes in sperm was analysed by immunocytochemistry and flow cytometry and correlated with morphological abnormalities. Immunofluorescent studies identified NOX5 in acrosomal, equatorial, post-acrosomal regions, the body and the tail of both normal and abnormal sperm. Teratozoospermic semen samples had higher percentages of NOX5-positive sperm and expressed more NOX5 (based on higher mean fluorescent intensity) than normal semen samples. Positive correlations were observed between abnormal sperm morphology and both the percentage of NOX5-positive sperm and the magnitude of NOX5 expression. Based on these findings, we can assume that there is a positive correlation between ROS generation in teratozoospermia and that in NOX5 expression.

Authors+Show Affiliations

Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23030296

Citation

Ghani, E, et al. "Expression of NOX5 in Human Teratozoospermia Compared to Normozoospermia." Andrologia, vol. 45, no. 5, 2013, pp. 351-6.
Ghani E, Keshtgar S, Habibagahi M, et al. Expression of NOX5 in human teratozoospermia compared to normozoospermia. Andrologia. 2013;45(5):351-6.
Ghani, E., Keshtgar, S., Habibagahi, M., Ghannadi, A., & Kazeroni, M. (2013). Expression of NOX5 in human teratozoospermia compared to normozoospermia. Andrologia, 45(5), pp. 351-6. doi:10.1111/and.12023.
Ghani E, et al. Expression of NOX5 in Human Teratozoospermia Compared to Normozoospermia. Andrologia. 2013;45(5):351-6. PubMed PMID: 23030296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of NOX5 in human teratozoospermia compared to normozoospermia. AU - Ghani,E, AU - Keshtgar,S, AU - Habibagahi,M, AU - Ghannadi,A, AU - Kazeroni,M, Y1 - 2012/10/03/ PY - 2012/08/07/accepted PY - 2012/10/4/entrez PY - 2012/10/4/pubmed PY - 2014/3/19/medline KW - Human KW - NADPH oxidase 5 KW - immunocytochemistry KW - normozoospermia KW - teratozoospermia SP - 351 EP - 6 JF - Andrologia JO - Andrologia VL - 45 IS - 5 N2 - Spermatozoa are capable of producing small amounts of reactive oxygen species (ROS), and sperm in teratozoospermia generate more ROS than sperm in normozoospermia. The source of ROS production in ejaculated human sperm has not been fully clarified. Recently, NADPH oxidase 5 (NOX5) was detected in human sperm, and ROS generation by this enzyme was reported. We investigated the magnitude of NOX5 expression in normozoospermic (n = 12) and teratozoospermic (n = 13) semen samples with different percentages of abnormal sperm. The existence of NOX5 enzymes in sperm was analysed by immunocytochemistry and flow cytometry and correlated with morphological abnormalities. Immunofluorescent studies identified NOX5 in acrosomal, equatorial, post-acrosomal regions, the body and the tail of both normal and abnormal sperm. Teratozoospermic semen samples had higher percentages of NOX5-positive sperm and expressed more NOX5 (based on higher mean fluorescent intensity) than normal semen samples. Positive correlations were observed between abnormal sperm morphology and both the percentage of NOX5-positive sperm and the magnitude of NOX5 expression. Based on these findings, we can assume that there is a positive correlation between ROS generation in teratozoospermia and that in NOX5 expression. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/23030296/Expression_of_NOX5_in_human_teratozoospermia_compared_to_normozoospermia_ L2 - https://doi.org/10.1111/and.12023 DB - PRIME DP - Unbound Medicine ER -