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Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Nov 01; 908:137-42.JC

Abstract

A simple, convenient and high selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) using water-compatible cyromazine-imprinted polymer as adsorbent was proposed for the rapid screening of melamine from bovine milk coupled with liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized by cyromazine as dummy template and reformative methanol-water system as reaction medium showed higher affinity and selectivity to melamine, and so they were applied as the specific dispersant of MSPD to extraction of melamine and simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.24-60.0μgg(-1) with the correlation coefficient of 0.9994. The recoveries of melamine at three spiked levels were ranged from 86.0 to 96.2% with the relative standard deviation (RSD)≤4.0%. This proposed MI-MSPD method combined the advantages of MSPD and MIPs, and could be used as an alternative tool for analyzing the residues of melamine in complex milk samples.

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Authors+Show Affiliations

Yan H
Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China. yanhongyuan@126.com
Cheng X
No affiliation info available
Sun N
No affiliation info available
Cai T
No affiliation info available
Wu R
No affiliation info available
Han K
No affiliation info available

MeSH

AbsorptionAcetonitrilesAnimalsCattleChromatography, High Pressure LiquidLinear ModelsMethanolMilkMolecular ImprintingReproducibility of ResultsSolid Phase ExtractionSolventsSpectrophotometry, UltravioletTriazinesWater

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23040986

Citation

Yan, Hongyuan, et al. "Rapid and Selective Screening of Melamine in Bovine Milk Using Molecularly Imprinted Matrix Solid-phase Dispersion Coupled With Liquid Chromatography-ultraviolet Detection." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 908, 2012, pp. 137-42.
Yan H, Cheng X, Sun N, et al. Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2012;908:137-42.
Yan, H., Cheng, X., Sun, N., Cai, T., Wu, R., & Han, K. (2012). Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 908, 137-42. https://doi.org/10.1016/j.jchromb.2012.09.022
Yan H, et al. Rapid and Selective Screening of Melamine in Bovine Milk Using Molecularly Imprinted Matrix Solid-phase Dispersion Coupled With Liquid Chromatography-ultraviolet Detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Nov 1;908:137-42. PubMed PMID: 23040986.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection. AU - Yan,Hongyuan, AU - Cheng,Xiaoling, AU - Sun,Ning, AU - Cai,Tianyu, AU - Wu,Ruijun, AU - Han,Kun, Y1 - 2012/09/19/ PY - 2012/08/13/received PY - 2012/09/05/revised PY - 2012/09/13/accepted PY - 2012/10/9/entrez PY - 2012/10/9/pubmed PY - 2013/5/10/medline SP - 137 EP - 42 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 908 N2 - A simple, convenient and high selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) using water-compatible cyromazine-imprinted polymer as adsorbent was proposed for the rapid screening of melamine from bovine milk coupled with liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized by cyromazine as dummy template and reformative methanol-water system as reaction medium showed higher affinity and selectivity to melamine, and so they were applied as the specific dispersant of MSPD to extraction of melamine and simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.24-60.0μgg(-1) with the correlation coefficient of 0.9994. The recoveries of melamine at three spiked levels were ranged from 86.0 to 96.2% with the relative standard deviation (RSD)≤4.0%. This proposed MI-MSPD method combined the advantages of MSPD and MIPs, and could be used as an alternative tool for analyzing the residues of melamine in complex milk samples. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/23040986/Rapid_and_selective_screening_of_melamine_in_bovine_milk_using_molecularly_imprinted_matrix_solid_phase_dispersion_coupled_with_liquid_chromatography_ultraviolet_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(12)00552-1 DB - PRIME DP - Unbound Medicine ER -
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