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Rough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams.
Vet Microbiol. 2013 Jan 25; 161(3-4):339-43.VM

Abstract

The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now the mechanism of bacterial persistence is not understood. Cytokine expression profiles were studied for 8 months in rams after being experimentally infected with the rough virulent strain of B. ovis (R-B. ovis) to study the pathogenesis of B. ovis and immune mechanism possibly associated to bacteria tropism and persistence. The messenger RNA (mRNA) expression levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12, interferon-γ (INF-γ) and tumour necrosis factor-α (TNF-α) cytokines were quantified by real-time quantitative RT-PCR (qRT-PCR) in reproductive tissues (epididymus, testicles, ampolae, vesicular glands and bulbourethral glands), and non-reproductive (liver, spleen and kidneys) tissues at 30, 60, 120 and 240 days post infection (dpi). During the acute phase of infection at 30 dpi, the host immune response was most notable demonstrating an up-regulation of several cytokines in reproductive tissues, including the epididymus (IL-6, IL-1β and IL-1α), testicles (INF-γ and IL-12), bulbourethral glands (IL-6 and TNF-α) and ampolae (INF-γ, IL-10, IL-1β and IL-1α). During the development of infection, cytokine gene expression levels decreased, providing evidence of immunosuppression and evidence of immune evasion that favoured persistence of chronic R-B. ovis infection. During the chronic phase of R-B. ovis infection (120 and 240 dpi), cytokine production was down-regulated in the epididymus (IL-1β and IL-1α), testicles (INF-γ and IL-12), and ampolae (INF-γ, IL-10, IL-1β and IL-1α), with the exception of the bulbourethral glands (IL-6 and TNF-α) and epididymus (IL-6); in these tissues, R-B. ovis infection resulted in up-regulation of the pro-inflammatory cytokine IL-6. Herein, we report cytokine expression profiles in tissues of rams experimentally infected with the rough strain of B. ovis, which are associated with bacterial persistence and macrophage activation.

Authors+Show Affiliations

Faculdade de Medicina Veterinária e Zootecnia, Botucatu, UNESP - Univ Estadual Paulista, Botucatu, CEP 18618-000, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23041076

Citation

Antunes, João Marcelo Azevedo de Paula, et al. "Rough Virulent Strain of Brucella Ovis Induces Pro- and Anti-inflammatory Cytokines in Reproductive Tissues in Experimentally Infected Rams." Veterinary Microbiology, vol. 161, no. 3-4, 2013, pp. 339-43.
Antunes JM, Allendorf SD, Appolinário CM, et al. Rough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams. Vet Microbiol. 2013;161(3-4):339-43.
Antunes, J. M., Allendorf, S. D., Appolinário, C. M., Cagnini, D. Q., Figueiredo, P. R., Júnior, J. B., Baños, J. V., Kocan, K. M., de la Fuente, J., & Megid, J. (2013). Rough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams. Veterinary Microbiology, 161(3-4), 339-43. https://doi.org/10.1016/j.vetmic.2012.07.024
Antunes JM, et al. Rough Virulent Strain of Brucella Ovis Induces Pro- and Anti-inflammatory Cytokines in Reproductive Tissues in Experimentally Infected Rams. Vet Microbiol. 2013 Jan 25;161(3-4):339-43. PubMed PMID: 23041076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rough virulent strain of Brucella ovis induces pro- and anti-inflammatory cytokines in reproductive tissues in experimentally infected rams. AU - Antunes,João Marcelo Azevedo de Paula, AU - Allendorf,Susan Dora, AU - Appolinário,Camila Michele, AU - Cagnini,Didier Quevedo, AU - Figueiredo,Paula Ripamonte, AU - Júnior,José Buratini, AU - Baños,Joaquin Vicente, AU - Kocan,Katherine M, AU - de la Fuente,José, AU - Megid,Jane, Y1 - 2012/08/25/ PY - 2012/04/12/received PY - 2012/07/14/revised PY - 2012/07/16/accepted PY - 2012/10/9/entrez PY - 2012/10/9/pubmed PY - 2013/8/6/medline SP - 339 EP - 43 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 161 IS - 3-4 N2 - The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now the mechanism of bacterial persistence is not understood. Cytokine expression profiles were studied for 8 months in rams after being experimentally infected with the rough virulent strain of B. ovis (R-B. ovis) to study the pathogenesis of B. ovis and immune mechanism possibly associated to bacteria tropism and persistence. The messenger RNA (mRNA) expression levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12, interferon-γ (INF-γ) and tumour necrosis factor-α (TNF-α) cytokines were quantified by real-time quantitative RT-PCR (qRT-PCR) in reproductive tissues (epididymus, testicles, ampolae, vesicular glands and bulbourethral glands), and non-reproductive (liver, spleen and kidneys) tissues at 30, 60, 120 and 240 days post infection (dpi). During the acute phase of infection at 30 dpi, the host immune response was most notable demonstrating an up-regulation of several cytokines in reproductive tissues, including the epididymus (IL-6, IL-1β and IL-1α), testicles (INF-γ and IL-12), bulbourethral glands (IL-6 and TNF-α) and ampolae (INF-γ, IL-10, IL-1β and IL-1α). During the development of infection, cytokine gene expression levels decreased, providing evidence of immunosuppression and evidence of immune evasion that favoured persistence of chronic R-B. ovis infection. During the chronic phase of R-B. ovis infection (120 and 240 dpi), cytokine production was down-regulated in the epididymus (IL-1β and IL-1α), testicles (INF-γ and IL-12), and ampolae (INF-γ, IL-10, IL-1β and IL-1α), with the exception of the bulbourethral glands (IL-6 and TNF-α) and epididymus (IL-6); in these tissues, R-B. ovis infection resulted in up-regulation of the pro-inflammatory cytokine IL-6. Herein, we report cytokine expression profiles in tissues of rams experimentally infected with the rough strain of B. ovis, which are associated with bacterial persistence and macrophage activation. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/23041076/Rough_virulent_strain_of_Brucella_ovis_induces_pro__and_anti_inflammatory_cytokines_in_reproductive_tissues_in_experimentally_infected_rams_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(12)00409-9 DB - PRIME DP - Unbound Medicine ER -