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Taurine attenuates methamphetamine-induced autophagy and apoptosis in PC12 cells through mTOR signaling pathway.
Toxicol Lett. 2012 Nov 23; 215(1):1-7.TL

Abstract

Methamphetamine (METH), a commonly abused psychostimulant, has been shown to induce neuronal damage by causing reactive oxygen species (ROS) formation, apoptosis and autophagy. Taurine (2-aminoethanesulfonic acid) is involved in several physiological actions in the brain, including neuroprotection, osmoregulation and neurotransmission. In this study, we investigate the protective effect of taurine against METH-induced neurotoxicity in PC12 cells and the underlying mechanism. The results showed that taurine significantly increased the cell viability inhibited by METH. LC3-II expression was elevated by METH treatment, whereas such increase was obviously attenuated by taurine. Co-treatment of taurine strongly reversed the decline of antioxidase activities induced by METH. Moreover, phosphorylated mammalian target of rapamycin (p-mTOR) was significantly inhibited by METH, whereas complementation of taurine markedly increased the expression of p-mTOR in PC12 cells, rather than phosphorylated Erk. Interestingly, taurine-induced decreasing expression of LC3-II was partially blocked by pretreatment of RAD001, an mTOR inhibitor. These results indicated that taurine inhibits METH-induced autophagic process through activating mTOR rather than Erk signaling. Collectively, our study shows that taurine protects METH-induced PC12 cells damage by attenuating ROS production, apoptosis and autophagy, at least in part, via mTOR signaling pathway.

Authors+Show Affiliations

National Chengdu Center for Safety Evaluation of Drugs, State Key Lab of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23041169

Citation

Li, Yan, et al. "Taurine Attenuates Methamphetamine-induced Autophagy and Apoptosis in PC12 Cells Through mTOR Signaling Pathway." Toxicology Letters, vol. 215, no. 1, 2012, pp. 1-7.
Li Y, Hu Z, Chen B, et al. Taurine attenuates methamphetamine-induced autophagy and apoptosis in PC12 cells through mTOR signaling pathway. Toxicol Lett. 2012;215(1):1-7.
Li, Y., Hu, Z., Chen, B., Bu, Q., Lu, W., Deng, Y., Zhu, R., Shao, X., Hou, J., Zhao, J., Li, H., Zhang, B., Huang, Y., Lv, L., Zhao, Y., & Cen, X. (2012). Taurine attenuates methamphetamine-induced autophagy and apoptosis in PC12 cells through mTOR signaling pathway. Toxicology Letters, 215(1), 1-7. https://doi.org/10.1016/j.toxlet.2012.09.019
Li Y, et al. Taurine Attenuates Methamphetamine-induced Autophagy and Apoptosis in PC12 Cells Through mTOR Signaling Pathway. Toxicol Lett. 2012 Nov 23;215(1):1-7. PubMed PMID: 23041169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Taurine attenuates methamphetamine-induced autophagy and apoptosis in PC12 cells through mTOR signaling pathway. AU - Li,Yan, AU - Hu,Zhengtao, AU - Chen,Bo, AU - Bu,Qian, AU - Lu,Wenjie, AU - Deng,Yi, AU - Zhu,Ruiming, AU - Shao,Xue, AU - Hou,Jing, AU - Zhao,Jinxuan, AU - Li,Hongyu, AU - Zhang,Baolai, AU - Huang,Yina, AU - Lv,Lei, AU - Zhao,Yinglan, AU - Cen,Xiaobo, Y1 - 2012/10/02/ PY - 2012/08/16/received PY - 2012/09/18/revised PY - 2012/09/25/accepted PY - 2012/10/9/entrez PY - 2012/10/9/pubmed PY - 2013/1/1/medline SP - 1 EP - 7 JF - Toxicology letters JO - Toxicol Lett VL - 215 IS - 1 N2 - Methamphetamine (METH), a commonly abused psychostimulant, has been shown to induce neuronal damage by causing reactive oxygen species (ROS) formation, apoptosis and autophagy. Taurine (2-aminoethanesulfonic acid) is involved in several physiological actions in the brain, including neuroprotection, osmoregulation and neurotransmission. In this study, we investigate the protective effect of taurine against METH-induced neurotoxicity in PC12 cells and the underlying mechanism. The results showed that taurine significantly increased the cell viability inhibited by METH. LC3-II expression was elevated by METH treatment, whereas such increase was obviously attenuated by taurine. Co-treatment of taurine strongly reversed the decline of antioxidase activities induced by METH. Moreover, phosphorylated mammalian target of rapamycin (p-mTOR) was significantly inhibited by METH, whereas complementation of taurine markedly increased the expression of p-mTOR in PC12 cells, rather than phosphorylated Erk. Interestingly, taurine-induced decreasing expression of LC3-II was partially blocked by pretreatment of RAD001, an mTOR inhibitor. These results indicated that taurine inhibits METH-induced autophagic process through activating mTOR rather than Erk signaling. Collectively, our study shows that taurine protects METH-induced PC12 cells damage by attenuating ROS production, apoptosis and autophagy, at least in part, via mTOR signaling pathway. SN - 1879-3169 UR - https://www.unboundmedicine.com/medline/citation/23041169/Taurine_attenuates_methamphetamine_induced_autophagy_and_apoptosis_in_PC12_cells_through_mTOR_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4274(12)01324-0 DB - PRIME DP - Unbound Medicine ER -