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Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant.
J Ethnopharmacol. 2012 Dec 18; 144(3):474-82.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Bauhinia platypetala Burch. is a traditionally used Brazilian medicinal plant, although no evidence in the literature substantiates the safety of its use.

AIM OF THE STUDY

The aim of this study was to investigate the safety of the ethanolic extract and the ethereal fraction of B. platypetala leaves.

MATERIALS AND METHODS

The identification of chemical compounds from the B. platypetala ethanolic extract and its ethereal fraction was performed by GC/MS and ESI-MS/MS. The plant's toxicological, cytotoxic, mutagenic and genotoxic properties were determined in Saccharomyces cerevisiae strains and V79 cell culture by survival assays and comet assay.

RESULTS

The major compound identified in the B. platypetala ethanolic extract is palmitic acid, kaempferitirin and quercitrin, while the B. platypetala ethereal fraction was found to be rich in phytol, gamma-sitosterol and vitamin E. Moreover, the results indicated that the B. platypetala ethanolic extract has an anti-oxidative effect against H(2)O(2) in yeast. In addition, the B. platypetala ethanolic extract did not induce mutagenic effects on the S. cerevisiae N123 strain, but the ethereal fraction of B. platypetala at higher concentrations (250-500 μg/mL) induced cytotoxicity and mutagenicity. A slight cytotoxic effect was observed in mammalian V79 cells; however, both the B. platypetala ethanolic extract and its ethereal fraction were able to induce DNA strand breaks in V79 cells, as detected by the alkaline comet assay.

CONCLUSION

The B. platypetala ethanolic extract has antioxidant action and showed absence of mutagenic effects in yeast S. cerevisiae. On the other hand B. platypetala ethereal fraction is mutagenic and does not show antioxidant activity in yeast. In mammalian cells B. platypetala ethanolic extract and it's ethereal fraction induce cyotoxic and genotoxic action.

Authors+Show Affiliations

Post-Graduation Program in Genetic and Applied Toxicology, Lutheran University of Brazil, Canoas 92425-900, RS, Brazil. borgissantos@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23041699

Citation

Santos, Francisco José Borges Dos, et al. "Genotoxic and Mutagenic Properties of Bauhinia Platypetala Extract, a Traditional Brazilian Medicinal Plant." Journal of Ethnopharmacology, vol. 144, no. 3, 2012, pp. 474-82.
Santos FJ, Moura DJ, Péres VF, et al. Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant. J Ethnopharmacol. 2012;144(3):474-82.
Santos, F. J., Moura, D. J., Péres, V. F., Sperotto, A. R., Caramão, E. B., Cavalcante, A. A., & Saffi, J. (2012). Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant. Journal of Ethnopharmacology, 144(3), 474-82. https://doi.org/10.1016/j.jep.2012.08.047
Santos FJ, et al. Genotoxic and Mutagenic Properties of Bauhinia Platypetala Extract, a Traditional Brazilian Medicinal Plant. J Ethnopharmacol. 2012 Dec 18;144(3):474-82. PubMed PMID: 23041699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant. AU - Santos,Francisco José Borges Dos, AU - Moura,Dinara Jaqueline, AU - Péres,Valéria Flores, AU - Sperotto,Angelo Regis de Moura, AU - Caramão,Elina Bastos, AU - Cavalcante,Ana Amélia de Carvalho Melo, AU - Saffi,Jenifer, Y1 - 2012/10/04/ PY - 2012/01/18/received PY - 2012/08/14/revised PY - 2012/08/25/accepted PY - 2012/10/9/entrez PY - 2012/10/9/pubmed PY - 2013/5/10/medline SP - 474 EP - 82 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 144 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Bauhinia platypetala Burch. is a traditionally used Brazilian medicinal plant, although no evidence in the literature substantiates the safety of its use. AIM OF THE STUDY: The aim of this study was to investigate the safety of the ethanolic extract and the ethereal fraction of B. platypetala leaves. MATERIALS AND METHODS: The identification of chemical compounds from the B. platypetala ethanolic extract and its ethereal fraction was performed by GC/MS and ESI-MS/MS. The plant's toxicological, cytotoxic, mutagenic and genotoxic properties were determined in Saccharomyces cerevisiae strains and V79 cell culture by survival assays and comet assay. RESULTS: The major compound identified in the B. platypetala ethanolic extract is palmitic acid, kaempferitirin and quercitrin, while the B. platypetala ethereal fraction was found to be rich in phytol, gamma-sitosterol and vitamin E. Moreover, the results indicated that the B. platypetala ethanolic extract has an anti-oxidative effect against H(2)O(2) in yeast. In addition, the B. platypetala ethanolic extract did not induce mutagenic effects on the S. cerevisiae N123 strain, but the ethereal fraction of B. platypetala at higher concentrations (250-500 μg/mL) induced cytotoxicity and mutagenicity. A slight cytotoxic effect was observed in mammalian V79 cells; however, both the B. platypetala ethanolic extract and its ethereal fraction were able to induce DNA strand breaks in V79 cells, as detected by the alkaline comet assay. CONCLUSION: The B. platypetala ethanolic extract has antioxidant action and showed absence of mutagenic effects in yeast S. cerevisiae. On the other hand B. platypetala ethereal fraction is mutagenic and does not show antioxidant activity in yeast. In mammalian cells B. platypetala ethanolic extract and it's ethereal fraction induce cyotoxic and genotoxic action. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23041699/Genotoxic_and_mutagenic_properties_of_Bauhinia_platypetala_extract_a_traditional_Brazilian_medicinal_plant_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00590-9 DB - PRIME DP - Unbound Medicine ER -