Tags

Type your tag names separated by a space and hit enter

Nano rolling-circle amplification for enhanced SERS hot spots in protein microarray analysis.
Anal Chem. 2012 Nov 06; 84(21):9139-45.AC

Abstract

Although "hot spots" have been proved to contribute to surface enhanced Raman scattering (SERS), less attention was paid to increase the number of the "hot spot" to directly enhance the Raman signals in bioanalytical systems. Here we report a new strategy based on nano rolling-circle amplification (nanoRCA) and nano hyperbranched rolling-circle amplification (nanoHRCA) to increase "hot spot" groups for protein microarrays. First, protein and ssDNA are coassembled on gold nanoparticles, making the assembled probe have both binding ability and hybridization ability. Second, the ssDNAs act as primers to initiate in situ RCA reaction to produced long ssDNAs. Third, a large number of SERS probes are loaded on the long ssDNA templetes, allowing thousands of SERS probes involved in each biomolecular recognition event. The strategy offered high-efficiency Raman enhancement and could detect less than 10 zeptomolar protein molecules in protein microarray analysis.

Authors+Show Affiliations

Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23046056

Citation

Yan, Juan, et al. "Nano Rolling-circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis." Analytical Chemistry, vol. 84, no. 21, 2012, pp. 9139-45.
Yan J, Su S, He S, et al. Nano rolling-circle amplification for enhanced SERS hot spots in protein microarray analysis. Anal Chem. 2012;84(21):9139-45.
Yan, J., Su, S., He, S., He, Y., Zhao, B., Wang, D., Zhang, H., Huang, Q., Song, S., & Fan, C. (2012). Nano rolling-circle amplification for enhanced SERS hot spots in protein microarray analysis. Analytical Chemistry, 84(21), 9139-45. https://doi.org/10.1021/ac301809e
Yan J, et al. Nano Rolling-circle Amplification for Enhanced SERS Hot Spots in Protein Microarray Analysis. Anal Chem. 2012 Nov 6;84(21):9139-45. PubMed PMID: 23046056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nano rolling-circle amplification for enhanced SERS hot spots in protein microarray analysis. AU - Yan,Juan, AU - Su,Shao, AU - He,Shijiang, AU - He,Yao, AU - Zhao,Bin, AU - Wang,Dongfang, AU - Zhang,Honglu, AU - Huang,Qing, AU - Song,Shiping, AU - Fan,Chunhai, Y1 - 2012/10/18/ PY - 2012/10/11/entrez PY - 2012/10/11/pubmed PY - 2013/12/16/medline SP - 9139 EP - 45 JF - Analytical chemistry JO - Anal Chem VL - 84 IS - 21 N2 - Although "hot spots" have been proved to contribute to surface enhanced Raman scattering (SERS), less attention was paid to increase the number of the "hot spot" to directly enhance the Raman signals in bioanalytical systems. Here we report a new strategy based on nano rolling-circle amplification (nanoRCA) and nano hyperbranched rolling-circle amplification (nanoHRCA) to increase "hot spot" groups for protein microarrays. First, protein and ssDNA are coassembled on gold nanoparticles, making the assembled probe have both binding ability and hybridization ability. Second, the ssDNAs act as primers to initiate in situ RCA reaction to produced long ssDNAs. Third, a large number of SERS probes are loaded on the long ssDNA templetes, allowing thousands of SERS probes involved in each biomolecular recognition event. The strategy offered high-efficiency Raman enhancement and could detect less than 10 zeptomolar protein molecules in protein microarray analysis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/23046056/Nano_rolling_circle_amplification_for_enhanced_SERS_hot_spots_in_protein_microarray_analysis_ L2 - https://doi.org/10.1021/ac301809e DB - PRIME DP - Unbound Medicine ER -