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Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat.
Chromosoma. 2012 Dec; 121(6):597-611.C

Abstract

Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation.

Authors+Show Affiliations

Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23052335

Citation

Danilova, Tatiana V., et al. "Single-copy Gene Fluorescence in Situ Hybridization and Genome Analysis: Acc-2 Loci Mark Evolutionary Chromosomal Rearrangements in Wheat." Chromosoma, vol. 121, no. 6, 2012, pp. 597-611.
Danilova TV, Friebe B, Gill BS. Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Chromosoma. 2012;121(6):597-611.
Danilova, T. V., Friebe, B., & Gill, B. S. (2012). Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Chromosoma, 121(6), 597-611. https://doi.org/10.1007/s00412-012-0384-7
Danilova TV, Friebe B, Gill BS. Single-copy Gene Fluorescence in Situ Hybridization and Genome Analysis: Acc-2 Loci Mark Evolutionary Chromosomal Rearrangements in Wheat. Chromosoma. 2012;121(6):597-611. PubMed PMID: 23052335.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. AU - Danilova,Tatiana V, AU - Friebe,Bernd, AU - Gill,Bikram S, Y1 - 2012/10/02/ PY - 2012/08/17/received PY - 2012/09/17/accepted PY - 2012/09/12/revised PY - 2012/10/12/entrez PY - 2012/10/12/pubmed PY - 2013/6/1/medline SP - 597 EP - 611 JF - Chromosoma JO - Chromosoma VL - 121 IS - 6 N2 - Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation. SN - 1432-0886 UR - https://www.unboundmedicine.com/medline/citation/23052335/Single_copy_gene_fluorescence_in_situ_hybridization_and_genome_analysis:_Acc_2_loci_mark_evolutionary_chromosomal_rearrangements_in_wheat_ L2 - https://dx.doi.org/10.1007/s00412-012-0384-7 DB - PRIME DP - Unbound Medicine ER -