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Cell type-specific regulation of inhibition via cannabinoid type 1 receptors in rat neocortex.
J Neurophysiol. 2013 Jan; 109(1):216-24.JN

Abstract

Endogenous cannabinoid type 1 (CB1) receptors demonstrate a cell type-specific expression and are potent modulators of synaptic transmission within the central nervous system. We aimed to investigate whether two classes of multipolar interneuron in the neocortex displayed a form of short-term synaptic plasticity, depolarization-induced suppression of inhibition (DSI), and whether the DSI was mediated by a common receptor. Paired whole cell recordings combined with biocytin labeling were performed between pyramidal cells and either multipolar adapting or multipolar nonadapting interneurons in layers II-IV of male Wistar rat (postnatal day 17-22) somatosensory cortex. Inhibitory postsynaptic potentials elicited by multipolar adapting interneurons were sensitive to DSI, which was blocked by the CB1 receptor antagonist AM-251 (8 μM), indicating that the suppression of inhibition was mediated by CB1 receptors. Two subpopulations of multipolar nonadapting interneuron-to-pyramidal cell connections were discovered on the basis of their susceptibility to DSI. Whereas 50% were insensitive to DSI, the remaining half were sensitive to DSI, which could not be prevented by AM-251. DSI at these connections was also insensitive to the group I (mGluRIa) and III metabotropic glutamate receptor antagonists (RS)-1-aminoindan-1,5-dicarboxylic acid (100 μM) and (RS)-α-cyclopropyl-4-phosphonophenylglycine (100 μM) and the group III agonist l-2-amino-4-phosphonobutanoate (50 μM). However, multipolar nonadapting interneuron-to-pyramidal cell connections were sensitive to the endocannabinoid anandamide (9 μM), mimicking the effects of DSI, which also could not be prevented by AM-251, implying a CB1 receptor-independent suppression of inhibition. These results reveal an interneuron type-specific modulation of synaptic transmission via CB receptors in the neocortex.

Authors+Show Affiliations

Department of Pharmacology, University College London School of Pharmacy, London, United Kingdom.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23054605

Citation

De-May, Claire L., and Afia B. Ali. "Cell Type-specific Regulation of Inhibition Via Cannabinoid Type 1 Receptors in Rat Neocortex." Journal of Neurophysiology, vol. 109, no. 1, 2013, pp. 216-24.
De-May CL, Ali AB. Cell type-specific regulation of inhibition via cannabinoid type 1 receptors in rat neocortex. J Neurophysiol. 2013;109(1):216-24.
De-May, C. L., & Ali, A. B. (2013). Cell type-specific regulation of inhibition via cannabinoid type 1 receptors in rat neocortex. Journal of Neurophysiology, 109(1), 216-24. https://doi.org/10.1152/jn.00272.2012
De-May CL, Ali AB. Cell Type-specific Regulation of Inhibition Via Cannabinoid Type 1 Receptors in Rat Neocortex. J Neurophysiol. 2013;109(1):216-24. PubMed PMID: 23054605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell type-specific regulation of inhibition via cannabinoid type 1 receptors in rat neocortex. AU - De-May,Claire L, AU - Ali,Afia B, Y1 - 2012/10/10/ PY - 2012/10/12/entrez PY - 2012/10/12/pubmed PY - 2013/6/6/medline SP - 216 EP - 24 JF - Journal of neurophysiology JO - J Neurophysiol VL - 109 IS - 1 N2 - Endogenous cannabinoid type 1 (CB1) receptors demonstrate a cell type-specific expression and are potent modulators of synaptic transmission within the central nervous system. We aimed to investigate whether two classes of multipolar interneuron in the neocortex displayed a form of short-term synaptic plasticity, depolarization-induced suppression of inhibition (DSI), and whether the DSI was mediated by a common receptor. Paired whole cell recordings combined with biocytin labeling were performed between pyramidal cells and either multipolar adapting or multipolar nonadapting interneurons in layers II-IV of male Wistar rat (postnatal day 17-22) somatosensory cortex. Inhibitory postsynaptic potentials elicited by multipolar adapting interneurons were sensitive to DSI, which was blocked by the CB1 receptor antagonist AM-251 (8 μM), indicating that the suppression of inhibition was mediated by CB1 receptors. Two subpopulations of multipolar nonadapting interneuron-to-pyramidal cell connections were discovered on the basis of their susceptibility to DSI. Whereas 50% were insensitive to DSI, the remaining half were sensitive to DSI, which could not be prevented by AM-251. DSI at these connections was also insensitive to the group I (mGluRIa) and III metabotropic glutamate receptor antagonists (RS)-1-aminoindan-1,5-dicarboxylic acid (100 μM) and (RS)-α-cyclopropyl-4-phosphonophenylglycine (100 μM) and the group III agonist l-2-amino-4-phosphonobutanoate (50 μM). However, multipolar nonadapting interneuron-to-pyramidal cell connections were sensitive to the endocannabinoid anandamide (9 μM), mimicking the effects of DSI, which also could not be prevented by AM-251, implying a CB1 receptor-independent suppression of inhibition. These results reveal an interneuron type-specific modulation of synaptic transmission via CB receptors in the neocortex. SN - 1522-1598 UR - https://www.unboundmedicine.com/medline/citation/23054605/Cell_type_specific_regulation_of_inhibition_via_cannabinoid_type_1_receptors_in_rat_neocortex_ L2 - https://journals.physiology.org/doi/10.1152/jn.00272.2012?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -