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Expression of Yes-associated protein in gastric adenocarcinoma and inhibitory effects of its knockdown on gastric cancer cell proliferation and metastasis.
Int J Immunopathol Pharmacol. 2012 Jul-Sep; 25(3):583-90.IJ

Abstract

Yes-associated protein (YAP) has been implicated as an oncogene in multiple human cancers. In the present study, human gastric adenocarcinoma tissues of different grades (N=78) were collected and the mRNA and protein expression of YAP and phosphorylated YAP (p-YAP) in gastric adenocarcinomas were evaluated using immunohistochemistry, Real-time PCR and Western blot assays. Then, human gastric cancer SGC-7901 cells were stably transfected with lentivirus-mediated YAP small hairpin RNA (shRNA). The expression levels of YAP, proliferating cell nuclear antigen (PCNA) and metalloproteinase-2 (MMP-2) were detected and the effects of shRNA-mediated knockdown of YAP on cell proliferation and metastasis were assessed in gastric cancer cells. As a result, the expression of YAP was observed in 69.23 percent gastric adenocarcinoma tissues, elevating with the ascending order of tumor malignancy. Knockdown of YAP could down-regulated the expression of PCNA and MMP-2, and inhibit the proliferation and metastasis of gastric cancer cells. In conclusion, YAP is strongly expressed in gastric adenocarcinomas, and knockdown of YAP may inhibit gastric cancer cell proliferation and metastasis through down-regulation of PCNA and MMP-2 expression, suggesting that YAP represents an important therapeutic target in human gastric cancer.

Authors+Show Affiliations

Department of Gastroenterology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23058008

Citation

Zhang, J, et al. "Expression of Yes-associated Protein in Gastric Adenocarcinoma and Inhibitory Effects of Its Knockdown On Gastric Cancer Cell Proliferation and Metastasis." International Journal of Immunopathology and Pharmacology, vol. 25, no. 3, 2012, pp. 583-90.
Zhang J, Xu ZP, Yang YC, et al. Expression of Yes-associated protein in gastric adenocarcinoma and inhibitory effects of its knockdown on gastric cancer cell proliferation and metastasis. Int J Immunopathol Pharmacol. 2012;25(3):583-90.
Zhang, J., Xu, Z. P., Yang, Y. C., Zhu, J. S., Zhou, Z., & Chen, W. X. (2012). Expression of Yes-associated protein in gastric adenocarcinoma and inhibitory effects of its knockdown on gastric cancer cell proliferation and metastasis. International Journal of Immunopathology and Pharmacology, 25(3), 583-90.
Zhang J, et al. Expression of Yes-associated Protein in Gastric Adenocarcinoma and Inhibitory Effects of Its Knockdown On Gastric Cancer Cell Proliferation and Metastasis. Int J Immunopathol Pharmacol. 2012;25(3):583-90. PubMed PMID: 23058008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of Yes-associated protein in gastric adenocarcinoma and inhibitory effects of its knockdown on gastric cancer cell proliferation and metastasis. AU - Zhang,J, AU - Xu,Z-P, AU - Yang,Y-C, AU - Zhu,J-S, AU - Zhou,Z, AU - Chen,W-X, PY - 2012/10/13/entrez PY - 2012/10/13/pubmed PY - 2012/12/12/medline SP - 583 EP - 90 JF - International journal of immunopathology and pharmacology JO - Int J Immunopathol Pharmacol VL - 25 IS - 3 N2 - Yes-associated protein (YAP) has been implicated as an oncogene in multiple human cancers. In the present study, human gastric adenocarcinoma tissues of different grades (N=78) were collected and the mRNA and protein expression of YAP and phosphorylated YAP (p-YAP) in gastric adenocarcinomas were evaluated using immunohistochemistry, Real-time PCR and Western blot assays. Then, human gastric cancer SGC-7901 cells were stably transfected with lentivirus-mediated YAP small hairpin RNA (shRNA). The expression levels of YAP, proliferating cell nuclear antigen (PCNA) and metalloproteinase-2 (MMP-2) were detected and the effects of shRNA-mediated knockdown of YAP on cell proliferation and metastasis were assessed in gastric cancer cells. As a result, the expression of YAP was observed in 69.23 percent gastric adenocarcinoma tissues, elevating with the ascending order of tumor malignancy. Knockdown of YAP could down-regulated the expression of PCNA and MMP-2, and inhibit the proliferation and metastasis of gastric cancer cells. In conclusion, YAP is strongly expressed in gastric adenocarcinomas, and knockdown of YAP may inhibit gastric cancer cell proliferation and metastasis through down-regulation of PCNA and MMP-2 expression, suggesting that YAP represents an important therapeutic target in human gastric cancer. SN - 0394-6320 UR - https://www.unboundmedicine.com/medline/citation/23058008/Expression_of_Yes_associated_protein_in_gastric_adenocarcinoma_and_inhibitory_effects_of_its_knockdown_on_gastric_cancer_cell_proliferation_and_metastasis_ L2 - http://journals.sagepub.com/doi/full/10.1177/039463201202500304?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -