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Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor.
Biosens Bioelectron. 2013 Mar 15; 41:532-7.BB

Abstract

In this paper, biofunctional magnetic beads were investigated for bacterial cells concentration in a nanoporous alumina membrane based immunosensor for ultra-sensitive detection of E. coli O157:H7. The specific antibody modified magnetic beads were used for concentration of E coli O157:H7 from samples in a small region to enhance sensitivity. The magnetic bead conjugated E. coli O157:H7 cells were then captured on the nanoporous alumina membrane with immobilized antibody via assembled PEG-silane linker. Scanning electron microscopy and fluorescent microscopy were used to demonstrate the magnetic bead-bacteria cell conjugation and bacteria cells magnetic concentration, respectively. Impedance spectroscopy was used to monitor the pure E coli O157:H7 cells and magnetic bead conjugated E coli O157:H7 cells binding on antibody immobilized nanoporous membrane with or without magnetic field. Compared with direct detection of pure bacteria cells, this method via magnetic bead conjugation and concentration demonstrated the ultrasensitivity of 10 CFU/mL for E coli O157:H7 detection.

Authors+Show Affiliations

Interdisciplinary Division of Biomedical Engineering, the Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23058659

Citation

Chan, Ka Yiu, et al. "Ultrasensitive Detection of E. Coli O157:H7 With Biofunctional Magnetic Bead Concentration Via Nanoporous Membrane Based Electrochemical Immunosensor." Biosensors & Bioelectronics, vol. 41, 2013, pp. 532-7.
Chan KY, Ye WW, Zhang Y, et al. Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor. Biosens Bioelectron. 2013;41:532-7.
Chan, K. Y., Ye, W. W., Zhang, Y., Xiao, L. D., Leung, P. H., Li, Y., & Yang, M. (2013). Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor. Biosensors & Bioelectronics, 41, 532-7. https://doi.org/10.1016/j.bios.2012.09.016
Chan KY, et al. Ultrasensitive Detection of E. Coli O157:H7 With Biofunctional Magnetic Bead Concentration Via Nanoporous Membrane Based Electrochemical Immunosensor. Biosens Bioelectron. 2013 Mar 15;41:532-7. PubMed PMID: 23058659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive detection of E. coli O157:H7 with biofunctional magnetic bead concentration via nanoporous membrane based electrochemical immunosensor. AU - Chan,Ka Yiu, AU - Ye,Wei Wei, AU - Zhang,Yu, AU - Xiao,Li Dan, AU - Leung,Polly H M, AU - Li,Yi, AU - Yang,Mo, Y1 - 2012/09/19/ PY - 2012/07/04/received PY - 2012/09/07/revised PY - 2012/09/12/accepted PY - 2012/10/13/entrez PY - 2012/10/13/pubmed PY - 2013/6/1/medline SP - 532 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 41 N2 - In this paper, biofunctional magnetic beads were investigated for bacterial cells concentration in a nanoporous alumina membrane based immunosensor for ultra-sensitive detection of E. coli O157:H7. The specific antibody modified magnetic beads were used for concentration of E coli O157:H7 from samples in a small region to enhance sensitivity. The magnetic bead conjugated E. coli O157:H7 cells were then captured on the nanoporous alumina membrane with immobilized antibody via assembled PEG-silane linker. Scanning electron microscopy and fluorescent microscopy were used to demonstrate the magnetic bead-bacteria cell conjugation and bacteria cells magnetic concentration, respectively. Impedance spectroscopy was used to monitor the pure E coli O157:H7 cells and magnetic bead conjugated E coli O157:H7 cells binding on antibody immobilized nanoporous membrane with or without magnetic field. Compared with direct detection of pure bacteria cells, this method via magnetic bead conjugation and concentration demonstrated the ultrasensitivity of 10 CFU/mL for E coli O157:H7 detection. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/23058659/Ultrasensitive_detection_of_E__coli_O157:H7_with_biofunctional_magnetic_bead_concentration_via_nanoporous_membrane_based_electrochemical_immunosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(12)00622-7 DB - PRIME DP - Unbound Medicine ER -