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Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck.
J Urol. 2013 Apr; 189(4):1567-73.JU

Abstract

PURPOSE

We investigated the possible involvement of H2S in nitric oxide independent inhibitory neurotransmission to the pig bladder neck.

MATERIALS AND METHODS

We used immunohistochemistry to determine the expression of the H2S synthesis enzymes cystathionine γ-lyase and cystathionine β-synthase. We also used electrical field stimulation and myographs for isometric force recordings to study relaxation in response to endogenously released or exogenously applied H2S in urothelium denuded, phenylephrine precontracted bladder neck strips under noradrenergic, noncholinergic, nonnitrergic conditions.

RESULTS

Cystathionine γ-lyase and cystathionine β-synthase expression was observed in nerve fibers in the smooth muscle layer. Cystathionine γ-lyase and cystathionine β-synthase immunoreactive fibers were also identified around the small arteries supplying the bladder neck. Electrical field stimulation (2 to 16 Hz) evoked frequency dependent relaxation, which was decreased by DL-propargylglycine and abolished by tetrodotoxin (blockers of cystathionine γ-lyase and neuronal voltage gated Na(+) channels, respectively). The cystathionine β-synthase inhibitor O-(carboxymethyl)hydroxylamine did not change nerve mediated responses. The H2S donor GYY4137 (0.1 nM to 10 μM) induced potent, concentration dependent relaxation, which was not modified by neuronal voltage gated Na(+) channels, or cystathionine γ-lyase or cystathionine β-synthase blockade.

CONCLUSIONS

Results suggest that endogenous H2S synthesized by cystathionine γ-lyase and released from intramural nerves acts as a powerful signaling molecule in nitric oxide independent inhibitory transmission to the pig bladder neck.

Authors+Show Affiliations

Departamento de Fisiología, Facultad de Farmacia, Universidad Complutense de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23063804

Citation

Fernandes, Vítor S., et al. "Endogenous Hydrogen Sulfide Has a Powerful Role in Inhibitory Neurotransmission to the Pig Bladder Neck." The Journal of Urology, vol. 189, no. 4, 2013, pp. 1567-73.
Fernandes VS, Ribeiro AS, Martínez MP, et al. Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. J Urol. 2013;189(4):1567-73.
Fernandes, V. S., Ribeiro, A. S., Martínez, M. P., Orensanz, L. M., Barahona, M. V., Martínez-Sáenz, A., Recio, P., Benedito, S., Bustamante, S., Carballido, J., García-Sacristán, A., Prieto, D., & Hernández, M. (2013). Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. The Journal of Urology, 189(4), 1567-73. https://doi.org/10.1016/j.juro.2012.10.006
Fernandes VS, et al. Endogenous Hydrogen Sulfide Has a Powerful Role in Inhibitory Neurotransmission to the Pig Bladder Neck. J Urol. 2013;189(4):1567-73. PubMed PMID: 23063804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. AU - Fernandes,Vítor S, AU - Ribeiro,Ana S F, AU - Martínez,María Pilar, AU - Orensanz,Luis M, AU - Barahona,María Victoria, AU - Martínez-Sáenz,Ana, AU - Recio,Paz, AU - Benedito,Sara, AU - Bustamante,Salvador, AU - Carballido,Joaquín, AU - García-Sacristán,Albino, AU - Prieto,Dolores, AU - Hernández,Medardo, Y1 - 2012/10/11/ PY - 2012/10/03/accepted PY - 2012/10/16/entrez PY - 2012/10/16/pubmed PY - 2013/7/9/medline SP - 1567 EP - 73 JF - The Journal of urology JO - J Urol VL - 189 IS - 4 N2 - PURPOSE: We investigated the possible involvement of H2S in nitric oxide independent inhibitory neurotransmission to the pig bladder neck. MATERIALS AND METHODS: We used immunohistochemistry to determine the expression of the H2S synthesis enzymes cystathionine γ-lyase and cystathionine β-synthase. We also used electrical field stimulation and myographs for isometric force recordings to study relaxation in response to endogenously released or exogenously applied H2S in urothelium denuded, phenylephrine precontracted bladder neck strips under noradrenergic, noncholinergic, nonnitrergic conditions. RESULTS: Cystathionine γ-lyase and cystathionine β-synthase expression was observed in nerve fibers in the smooth muscle layer. Cystathionine γ-lyase and cystathionine β-synthase immunoreactive fibers were also identified around the small arteries supplying the bladder neck. Electrical field stimulation (2 to 16 Hz) evoked frequency dependent relaxation, which was decreased by DL-propargylglycine and abolished by tetrodotoxin (blockers of cystathionine γ-lyase and neuronal voltage gated Na(+) channels, respectively). The cystathionine β-synthase inhibitor O-(carboxymethyl)hydroxylamine did not change nerve mediated responses. The H2S donor GYY4137 (0.1 nM to 10 μM) induced potent, concentration dependent relaxation, which was not modified by neuronal voltage gated Na(+) channels, or cystathionine γ-lyase or cystathionine β-synthase blockade. CONCLUSIONS: Results suggest that endogenous H2S synthesized by cystathionine γ-lyase and released from intramural nerves acts as a powerful signaling molecule in nitric oxide independent inhibitory transmission to the pig bladder neck. SN - 1527-3792 UR - https://www.unboundmedicine.com/medline/citation/23063804/Endogenous_hydrogen_sulfide_has_a_powerful_role_in_inhibitory_neurotransmission_to_the_pig_bladder_neck_ L2 - https://www.jurology.com/doi/10.1016/j.juro.2012.10.006?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -