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Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents.
J Ethnopharmacol. 2012 Dec 18; 144(3):638-45.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Tibetan medicine get used to use the flowers of Gentiana straminea Maxim. to cure inflammation of stomach and intestines, hepatitis, cholecystitis, etc. The flowers of Gentiana macrophylla Pall. have been traditionally treated as an anti-inflammatory agent to clear heat in Mongolian medicine. In traditional Chinese medicine, Gentiana macrophylla Pall. and Gentiana straminea Maxim. have also been used under the name "Gentianae Macrophyllae Radix" and prescribed for the treatment of pain and inflammatory conditions.

AIM OF STUDY

The present study evaluated the pharmacological effects of two species of "Radix Gentianae Macrophyllae" in experimental inflammation and pain models, and determined the chemical compounds that may correlate with their pharmacological activities. The comparison is needed to identify whether the two related plants can be used interchangeably.

MATERIALS AND METHODS

We evaluated the pharmacological effects of the flowers of Gentiana macrophylla Pall. and Gentiana straminea Maxim. in experimental inflammation and pain models. An HPLC-MS method was developed to analyze the chemical composition. The effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim. on the p65 and p50 phosphorylation were examined by immunblotting. NF-κB transcriptional activity was measured using the luciferase assay, in vitro kinase assay and Griess reaction.

RESULTS

The extracts of Gentiana macrophylla Pall. and Gentiana straminea Maxim. possessed significant antinociceptive and anti-inflammatory activities. Flavonoids, secoiridoid glycosides and triterpines were determined in the extracts and may be the basis of the observed pharmacological effects. Nuclear translocation of p65, p50 and NF-κB transcriptional activity induced by LPS were suppressed by Gentiana macrophylla Pall. and Gentiana straminea Maxim.

CONCLUSION

The results clearly demonstrated that the chemical composition and pharmacological activities of the two herbs were similar, which support the interchangeability among the two herbs when using them in folk medicine.

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Publisher Full Text

Authors+Show Affiliations

Jia N
Department of Pharmacy, Xijing Hospital of the Fourth Military Medical University, Xi'an 710032, PR China.
Li Y
No affiliation info available
Wu Y
No affiliation info available
Xi M
No affiliation info available
Hur G
No affiliation info available
Zhang X
No affiliation info available
Cui J
No affiliation info available
Sun W
No affiliation info available
Wen A
No affiliation info available

MeSH

Acetic AcidAnalgesicsAnimalsAnti-Inflammatory AgentsCarrageenanCell LineEdemaFemaleGentianaHot TemperatureMaleMiceMice, Inbred ICRNF-kappa B p50 SubunitPainPhytotherapyPlant ExtractsRatsRats, Sprague-DawleyTranscription Factor RelAXylenes

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23063959

Citation

Jia, Na, et al. "Comparison of the Anti-inflammatory and Analgesic Effects of Gentiana Macrophylla Pall. and Gentiana Straminea Maxim., and Identification of Their Active Constituents." Journal of Ethnopharmacology, vol. 144, no. 3, 2012, pp. 638-45.
Jia N, Li Y, Wu Y, et al. Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents. J Ethnopharmacol. 2012;144(3):638-45.
Jia, N., Li, Y., Wu, Y., Xi, M., Hur, G., Zhang, X., Cui, J., Sun, W., & Wen, A. (2012). Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents. Journal of Ethnopharmacology, 144(3), 638-45. https://doi.org/10.1016/j.jep.2012.10.004
Jia N, et al. Comparison of the Anti-inflammatory and Analgesic Effects of Gentiana Macrophylla Pall. and Gentiana Straminea Maxim., and Identification of Their Active Constituents. J Ethnopharmacol. 2012 Dec 18;144(3):638-45. PubMed PMID: 23063959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents. AU - Jia,Na, AU - Li,Yuwen, AU - Wu,Yin, AU - Xi,Miaomiao, AU - Hur,Gangmin, AU - Zhang,Xinxin, AU - Cui,Jia, AU - Sun,Wenji, AU - Wen,Aidong, Y1 - 2012/10/12/ PY - 2012/05/30/received PY - 2012/09/25/revised PY - 2012/10/03/accepted PY - 2012/10/16/entrez PY - 2012/10/16/pubmed PY - 2013/5/10/medline SP - 638 EP - 45 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 144 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Tibetan medicine get used to use the flowers of Gentiana straminea Maxim. to cure inflammation of stomach and intestines, hepatitis, cholecystitis, etc. The flowers of Gentiana macrophylla Pall. have been traditionally treated as an anti-inflammatory agent to clear heat in Mongolian medicine. In traditional Chinese medicine, Gentiana macrophylla Pall. and Gentiana straminea Maxim. have also been used under the name "Gentianae Macrophyllae Radix" and prescribed for the treatment of pain and inflammatory conditions. AIM OF STUDY: The present study evaluated the pharmacological effects of two species of "Radix Gentianae Macrophyllae" in experimental inflammation and pain models, and determined the chemical compounds that may correlate with their pharmacological activities. The comparison is needed to identify whether the two related plants can be used interchangeably. MATERIALS AND METHODS: We evaluated the pharmacological effects of the flowers of Gentiana macrophylla Pall. and Gentiana straminea Maxim. in experimental inflammation and pain models. An HPLC-MS method was developed to analyze the chemical composition. The effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim. on the p65 and p50 phosphorylation were examined by immunblotting. NF-κB transcriptional activity was measured using the luciferase assay, in vitro kinase assay and Griess reaction. RESULTS: The extracts of Gentiana macrophylla Pall. and Gentiana straminea Maxim. possessed significant antinociceptive and anti-inflammatory activities. Flavonoids, secoiridoid glycosides and triterpines were determined in the extracts and may be the basis of the observed pharmacological effects. Nuclear translocation of p65, p50 and NF-κB transcriptional activity induced by LPS were suppressed by Gentiana macrophylla Pall. and Gentiana straminea Maxim. CONCLUSION: The results clearly demonstrated that the chemical composition and pharmacological activities of the two herbs were similar, which support the interchangeability among the two herbs when using them in folk medicine. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23063959/Comparison_of_the_anti_inflammatory_and_analgesic_effects_of_Gentiana_macrophylla_Pall__and_Gentiana_straminea_Maxim__and_identification_of_their_active_constituents_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00675-7 DB - PRIME DP - Unbound Medicine ER -