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Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties.
World J Microbiol Biotechnol. 2013 Feb; 29(2):355-64.WJ

Abstract

Entomopathogenic nematodes (EPN) are well-known as biological control agents and are found to have associated bacteria which can produce a wide range of bioactive secondary metabolites. We report herewith isolation of six proline containing cyclic dipeptides cyclo(D-Pro-L-Leu), cyclo(L-Pro-L-Met), cyclo(D-Pro-L-Phe), cyclo(L-Pro-L-Phe), cyclo(L-Pro-L-Tyr) and cyclo(L-Pro-D-Tyr) from ethyl acetate extract of the Luria Broth (LB) cell free culture filtrate of Bacillus sp. strain N associated with a new EPN Rhabditis sp. from sweet potato weevil grubs collected from Central Tuber Crops Research Institute farm. Antimicrobial studies of these 2,5-diketopiperazines (DKPs) against both medicinally and agriculturally important bacterium and fungi showed potent inhibitory values in the range of μg/mL. Cyclic dipeptides showed significantly higher activity than the commercial fungicide bavistin against agriculturally important fungi, viz., Fusarium oxysporum, Rhizoctonia solani, and Pencillium expansum. The highest activity of 2 μg/mL by cyclo(L-Pro-L-Phe) was recorded against P. expansum, a plant pathogen responsible for causing post harvest decay of stored apples and oranges. To our knowledge, this is the first report on the isolation of these DKPs from Rhabditis EPN bacterial strain Bacillus sp.

Authors+Show Affiliations

Division of Crop Protection/Division of Crop Utilisation, Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23065379

Citation

Kumar, Nishanth, et al. "Isolation of Proline-based Cyclic Dipeptides From Bacillus Sp. N Strain Associated With Rhabditid [corrected] Entomopathogenic Nematode and Its Antimicrobial Properties." World Journal of Microbiology & Biotechnology, vol. 29, no. 2, 2013, pp. 355-64.
Kumar N, Mohandas C, Nambisan B, et al. Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties. World J Microbiol Biotechnol. 2013;29(2):355-64.
Kumar, N., Mohandas, C., Nambisan, B., Kumar, D. R., & Lankalapalli, R. S. (2013). Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties. World Journal of Microbiology & Biotechnology, 29(2), 355-64. https://doi.org/10.1007/s11274-012-1189-9
Kumar N, et al. Isolation of Proline-based Cyclic Dipeptides From Bacillus Sp. N Strain Associated With Rhabditid [corrected] Entomopathogenic Nematode and Its Antimicrobial Properties. World J Microbiol Biotechnol. 2013;29(2):355-64. PubMed PMID: 23065379.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation of proline-based cyclic dipeptides from Bacillus sp. N strain associated with rhabditid [corrected] entomopathogenic nematode and its antimicrobial properties. AU - Kumar,Nishanth, AU - Mohandas,C, AU - Nambisan,Bala, AU - Kumar,D R Soban, AU - Lankalapalli,Ravi S, Y1 - 2012/10/12/ PY - 2012/07/09/received PY - 2012/10/06/accepted PY - 2012/10/16/entrez PY - 2012/10/16/pubmed PY - 2013/6/12/medline SP - 355 EP - 64 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 29 IS - 2 N2 - Entomopathogenic nematodes (EPN) are well-known as biological control agents and are found to have associated bacteria which can produce a wide range of bioactive secondary metabolites. We report herewith isolation of six proline containing cyclic dipeptides cyclo(D-Pro-L-Leu), cyclo(L-Pro-L-Met), cyclo(D-Pro-L-Phe), cyclo(L-Pro-L-Phe), cyclo(L-Pro-L-Tyr) and cyclo(L-Pro-D-Tyr) from ethyl acetate extract of the Luria Broth (LB) cell free culture filtrate of Bacillus sp. strain N associated with a new EPN Rhabditis sp. from sweet potato weevil grubs collected from Central Tuber Crops Research Institute farm. Antimicrobial studies of these 2,5-diketopiperazines (DKPs) against both medicinally and agriculturally important bacterium and fungi showed potent inhibitory values in the range of μg/mL. Cyclic dipeptides showed significantly higher activity than the commercial fungicide bavistin against agriculturally important fungi, viz., Fusarium oxysporum, Rhizoctonia solani, and Pencillium expansum. The highest activity of 2 μg/mL by cyclo(L-Pro-L-Phe) was recorded against P. expansum, a plant pathogen responsible for causing post harvest decay of stored apples and oranges. To our knowledge, this is the first report on the isolation of these DKPs from Rhabditis EPN bacterial strain Bacillus sp. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/23065379/Isolation_of_proline_based_cyclic_dipeptides_from_Bacillus_sp__N_strain_associated_with_rhabditid_[corrected]_entomopathogenic_nematode_and_its_antimicrobial_properties_ L2 - https://dx.doi.org/10.1007/s11274-012-1189-9 DB - PRIME DP - Unbound Medicine ER -