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Characterization of bifidobacteria in the digestive tract of the Japanese honeybee, Apis cerana japonica.
J Invertebr Pathol. 2013 Jan; 112(1):88-93.JI

Abstract

Bifidobacteria were isolated from the intestinal tract of the Japanese honeybee, Apis cerana japonica, and investigated for potential application as a probiotic agent against Melissococcus plutonius, the causal agent of European foulbrood (EFB), based on the findings of in vitro inhibition assays. A total of 11 bifidobacteria strains (designated as AcjBF1-AcjBF11) were isolated using a culture-dependent method and their 16S rRNA gene sequences were analyzed. The AcjBF isolates belonged to three distinct bifidobacterial phylotypes that were similar to those found in the European honeybee, Apis mellifera. Although the Japanese and European honeybees are distinct species with different traits and habits, the observation that they share highly similar bifidobacterial phylotypes suggests that bifidobacteria are conserved among honeybee species. Despite having extremely high 16S rRNA gene sequence similarities, the AcjBF isolates had markedly different carbohydrate fermentation profiles. In addition, in vitro growth inhibition assays revealed that the cell-free supernatants of all AcjBF isolates exhibited antagonistic effects on M. plutonius growth. These results indicate that the bifidobacteria isolated from the gut of Japanese honeybee could potentially be employed as a new biological agent to control EFB.

Authors+Show Affiliations

Honeybee Research Unit, Animal Breeding and Reproduction Division, NARO Institute of Livestock and Grassland Science, 2 Ikenodai, Tsukuba, Ibaraki 305-0901, Japan. s0930286@u.tsukuba.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23069130

Citation

Wu, Meihua, et al. "Characterization of Bifidobacteria in the Digestive Tract of the Japanese Honeybee, Apis Cerana Japonica." Journal of Invertebrate Pathology, vol. 112, no. 1, 2013, pp. 88-93.
Wu M, Sugimura Y, Takaya N, et al. Characterization of bifidobacteria in the digestive tract of the Japanese honeybee, Apis cerana japonica. J Invertebr Pathol. 2013;112(1):88-93.
Wu, M., Sugimura, Y., Takaya, N., Takamatsu, D., Kobayashi, M., Taylor, D., & Yoshiyama, M. (2013). Characterization of bifidobacteria in the digestive tract of the Japanese honeybee, Apis cerana japonica. Journal of Invertebrate Pathology, 112(1), 88-93. https://doi.org/10.1016/j.jip.2012.09.005
Wu M, et al. Characterization of Bifidobacteria in the Digestive Tract of the Japanese Honeybee, Apis Cerana Japonica. J Invertebr Pathol. 2013;112(1):88-93. PubMed PMID: 23069130.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of bifidobacteria in the digestive tract of the Japanese honeybee, Apis cerana japonica. AU - Wu,Meihua, AU - Sugimura,Yuya, AU - Takaya,Noriko, AU - Takamatsu,Daisuke, AU - Kobayashi,Masaru, AU - Taylor,DeMar, AU - Yoshiyama,Mikio, Y1 - 2012/10/12/ PY - 2012/04/10/received PY - 2012/09/11/revised PY - 2012/09/18/accepted PY - 2012/10/17/entrez PY - 2012/10/17/pubmed PY - 2013/11/20/medline SP - 88 EP - 93 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 112 IS - 1 N2 - Bifidobacteria were isolated from the intestinal tract of the Japanese honeybee, Apis cerana japonica, and investigated for potential application as a probiotic agent against Melissococcus plutonius, the causal agent of European foulbrood (EFB), based on the findings of in vitro inhibition assays. A total of 11 bifidobacteria strains (designated as AcjBF1-AcjBF11) were isolated using a culture-dependent method and their 16S rRNA gene sequences were analyzed. The AcjBF isolates belonged to three distinct bifidobacterial phylotypes that were similar to those found in the European honeybee, Apis mellifera. Although the Japanese and European honeybees are distinct species with different traits and habits, the observation that they share highly similar bifidobacterial phylotypes suggests that bifidobacteria are conserved among honeybee species. Despite having extremely high 16S rRNA gene sequence similarities, the AcjBF isolates had markedly different carbohydrate fermentation profiles. In addition, in vitro growth inhibition assays revealed that the cell-free supernatants of all AcjBF isolates exhibited antagonistic effects on M. plutonius growth. These results indicate that the bifidobacteria isolated from the gut of Japanese honeybee could potentially be employed as a new biological agent to control EFB. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/23069130/Characterization_of_bifidobacteria_in_the_digestive_tract_of_the_Japanese_honeybee_Apis_cerana_japonica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(12)00243-1 DB - PRIME DP - Unbound Medicine ER -