Tags

Type your tag names separated by a space and hit enter

Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa.
Mol Reprod Dev. 2012 Dec; 79(12):832-42.MR

Abstract

EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's expression and cellular distribution in rat tissues and its in vivo regulation by androgens in the epididymis. EPPIN (mRNA and protein) was abundantly expressed in the rat testis and epididymis; we also found that the vas deferens, seminal vesicles, and brain were novel sites of EPPIN expression. PCR studies demonstrated that in addition to Sertoli cells, spermatogenic cells expressed Eppin mRNA. EPPIN was immunolocalized in Sertoli cells and spermatogenic cells (pachytene spermatocytes and round and elongated spermatids) and in epithelial cells and spermatozoa from efferent ductules and epididymis. EPPIN staining was observed on the middle and principal pieces of the flagellum of testicular spermatozoa. Epididymal spermatozoa had more intense EPPIN staining on the flagellum, and the EPPIN staining became apparent on the head and neck regions. This suggested that the EPPIN found on maturing spermatozoa was secreted primarily by the epithelial cells of the epididymis. Surgical castration down-regulated EPPIN expression levels (mRNA and protein) in the caput and cauda epididymis, an effect reversed by testosterone replacement. Altogether, our data suggested that EPPIN expression in rats is more widespread than in humans and mice, and is androgen-dependent in the epididymis. This species could be used as an experimental model to further study EPPIN's role in male fertility.

Authors+Show Affiliations

Section of Experimental Endocrinology, Department of Pharmacology, Universidade Federal de São Paulo - Escola Paulista de Medicina, SP, Brazil. ejrsilva81@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23070980

Citation

Silva, Erick J R., et al. "Epididymal Protease Inhibitor (EPPIN) Is Differentially Expressed in the Male Rat Reproductive Tract and Immunolocalized in Maturing Spermatozoa." Molecular Reproduction and Development, vol. 79, no. 12, 2012, pp. 832-42.
Silva EJ, Patrão MT, Tsuruta JK, et al. Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa. Mol Reprod Dev. 2012;79(12):832-42.
Silva, E. J., Patrão, M. T., Tsuruta, J. K., O'Rand, M. G., & Avellar, M. C. (2012). Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa. Molecular Reproduction and Development, 79(12), 832-42. https://doi.org/10.1002/mrd.22119
Silva EJ, et al. Epididymal Protease Inhibitor (EPPIN) Is Differentially Expressed in the Male Rat Reproductive Tract and Immunolocalized in Maturing Spermatozoa. Mol Reprod Dev. 2012;79(12):832-42. PubMed PMID: 23070980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa. AU - Silva,Erick J R, AU - Patrão,Marília T C C, AU - Tsuruta,James K, AU - O'Rand,Michael G, AU - Avellar,Maria Christina W, Y1 - 2012/11/07/ PY - 2012/08/27/received PY - 2012/09/28/accepted PY - 2012/10/17/entrez PY - 2012/10/17/pubmed PY - 2013/6/21/medline SP - 832 EP - 42 JF - Molecular reproduction and development JO - Mol. Reprod. Dev. VL - 79 IS - 12 N2 - EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's expression and cellular distribution in rat tissues and its in vivo regulation by androgens in the epididymis. EPPIN (mRNA and protein) was abundantly expressed in the rat testis and epididymis; we also found that the vas deferens, seminal vesicles, and brain were novel sites of EPPIN expression. PCR studies demonstrated that in addition to Sertoli cells, spermatogenic cells expressed Eppin mRNA. EPPIN was immunolocalized in Sertoli cells and spermatogenic cells (pachytene spermatocytes and round and elongated spermatids) and in epithelial cells and spermatozoa from efferent ductules and epididymis. EPPIN staining was observed on the middle and principal pieces of the flagellum of testicular spermatozoa. Epididymal spermatozoa had more intense EPPIN staining on the flagellum, and the EPPIN staining became apparent on the head and neck regions. This suggested that the EPPIN found on maturing spermatozoa was secreted primarily by the epithelial cells of the epididymis. Surgical castration down-regulated EPPIN expression levels (mRNA and protein) in the caput and cauda epididymis, an effect reversed by testosterone replacement. Altogether, our data suggested that EPPIN expression in rats is more widespread than in humans and mice, and is androgen-dependent in the epididymis. This species could be used as an experimental model to further study EPPIN's role in male fertility. SN - 1098-2795 UR - https://www.unboundmedicine.com/medline/citation/23070980/Epididymal_protease_inhibitor__EPPIN__is_differentially_expressed_in_the_male_rat_reproductive_tract_and_immunolocalized_in_maturing_spermatozoa_ L2 - https://doi.org/10.1002/mrd.22119 DB - PRIME DP - Unbound Medicine ER -