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New genotypes of Orientia tsutsugamushi isolated from humans in Eastern Taiwan.
PLoS One. 2012; 7(10):e46997.Plos

Abstract

Scrub typhus, an acute febrile illness, is caused by the obligate intracellular bacterium Orientia tsutsugamushi. In our study, O. tsutsugamushi was rapidly detected and typed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 56-kDa type-specific antigen (TSA) gene. To investigate the genotypes of clinical variants of O. tsutsugamushi, we collected 3223 blood samples from eastern Taiwanese patients with suspected scrub typhus from 2002 to 2008. In total, 505 samples were found to be positive for scrub typhus infection by PCR, and bacteria were isolated from 282 of them. Four prototype genotype strains (Karp, Kato, Kawasaki and Gilliam) and eleven different Taiwanese genotype isolates (Taiwan-A, -B, -C, -D, -E, -G, -H, -J, -N, -O and -P) were identified by RPLF analysis. Taiwan-H, the major genotype in eastern Taiwan, exhibited prevalence and isolation rates of 47.3% (239/505) and 42.6% (120/282), respectively. We also assessed the genetic relatedness of the 56-kDa TSA gene among eight Taiwan-H isolates, thirteen other Taiwanese isolates and 104 DNA sequences deposited in the GenBank database using MEGA version 5.0 and PHYLIP version 3.66. We found that the Taiwan-H isolates formed into a new cluster, which was designated the Taiwan Gilliam-variant (TG-v) cluster to distinguish it from the Japanese Gilliam-variant (JG-v) cluster. According to Simplot analysis, TG-v is a new recombinant strain among Gilliam, Ikeda and Kato. Moreover, the Gilliam-Kawasaki cluster had the highest percentage of RFLP cases and was the most frequently isolated type in eastern Taiwan (50.1%, 253/505; 44.0%, 124/282). These findings shed light on the genetic evolution of O. tsutsugamushi into different strains and may be useful in vaccine development and epidemic disease control in the future.

Authors+Show Affiliations

Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23071693

Citation

Yang, Hui-Hua, et al. "New Genotypes of Orientia Tsutsugamushi Isolated From Humans in Eastern Taiwan." PloS One, vol. 7, no. 10, 2012, pp. e46997.
Yang HH, Huang IT, Lin CH, et al. New genotypes of Orientia tsutsugamushi isolated from humans in Eastern Taiwan. PLoS One. 2012;7(10):e46997.
Yang, H. H., Huang, I. T., Lin, C. H., Chen, T. Y., & Chen, L. K. (2012). New genotypes of Orientia tsutsugamushi isolated from humans in Eastern Taiwan. PloS One, 7(10), e46997. https://doi.org/10.1371/journal.pone.0046997
Yang HH, et al. New Genotypes of Orientia Tsutsugamushi Isolated From Humans in Eastern Taiwan. PLoS One. 2012;7(10):e46997. PubMed PMID: 23071693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - New genotypes of Orientia tsutsugamushi isolated from humans in Eastern Taiwan. AU - Yang,Hui-Hua, AU - Huang,I-Tsong, AU - Lin,Chin-Hui, AU - Chen,Tren-Yi, AU - Chen,Li-Kuang, Y1 - 2012/10/10/ PY - 2012/01/18/received PY - 2012/09/11/accepted PY - 2012/10/17/entrez PY - 2012/10/17/pubmed PY - 2013/5/2/medline SP - e46997 EP - e46997 JF - PloS one JO - PLoS One VL - 7 IS - 10 N2 - Scrub typhus, an acute febrile illness, is caused by the obligate intracellular bacterium Orientia tsutsugamushi. In our study, O. tsutsugamushi was rapidly detected and typed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 56-kDa type-specific antigen (TSA) gene. To investigate the genotypes of clinical variants of O. tsutsugamushi, we collected 3223 blood samples from eastern Taiwanese patients with suspected scrub typhus from 2002 to 2008. In total, 505 samples were found to be positive for scrub typhus infection by PCR, and bacteria were isolated from 282 of them. Four prototype genotype strains (Karp, Kato, Kawasaki and Gilliam) and eleven different Taiwanese genotype isolates (Taiwan-A, -B, -C, -D, -E, -G, -H, -J, -N, -O and -P) were identified by RPLF analysis. Taiwan-H, the major genotype in eastern Taiwan, exhibited prevalence and isolation rates of 47.3% (239/505) and 42.6% (120/282), respectively. We also assessed the genetic relatedness of the 56-kDa TSA gene among eight Taiwan-H isolates, thirteen other Taiwanese isolates and 104 DNA sequences deposited in the GenBank database using MEGA version 5.0 and PHYLIP version 3.66. We found that the Taiwan-H isolates formed into a new cluster, which was designated the Taiwan Gilliam-variant (TG-v) cluster to distinguish it from the Japanese Gilliam-variant (JG-v) cluster. According to Simplot analysis, TG-v is a new recombinant strain among Gilliam, Ikeda and Kato. Moreover, the Gilliam-Kawasaki cluster had the highest percentage of RFLP cases and was the most frequently isolated type in eastern Taiwan (50.1%, 253/505; 44.0%, 124/282). These findings shed light on the genetic evolution of O. tsutsugamushi into different strains and may be useful in vaccine development and epidemic disease control in the future. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23071693/New_genotypes_of_Orientia_tsutsugamushi_isolated_from_humans_in_Eastern_Taiwan_ L2 - https://dx.plos.org/10.1371/journal.pone.0046997 DB - PRIME DP - Unbound Medicine ER -