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Connective tissue growth factor is a positive regulator of epithelial-mesenchymal transition and promotes the adhesion with gastric cancer cells in human peritoneal mesothelial cells.
Cytokine. 2013 Jan; 61(1):173-80.C

Abstract

Connective tissue growth factor (CTGF) is involved in human cancer development and progression. Epithelial to mesenchymal transition (EMT) plays an important role in many biological processes. In this study, we wished to investigate the role of CTGF in EMT of peritoneal mesothelial cells and the effects of CTGF on adhesion of gastric cancer cells to mesothelial cells. Human peritoneal mesothelial cells (HPMCs) were cultured with TGF-β1 or various concentrations of CTGF for different time. The EMT process was monitored by morphology. Real-time RT-PCR and Western blot were used to evaluate the expression of vimentin, α-SMA , E-cadherin and β-catenin. RNA interference was used to achieve selective and specific knockdown of CTGF. We demonstrated that CTGF induced EMT of mesothelial cells in a dose- and time-dependent manner. HPMCs were exposed to TGF-β1 also underwent EMT which was associated with the induction of CTGF expression. Transfection with CTGF siRNA was able to reverse the EMT partially after treatment of TGF-β1. Moreover, the induced EMT of HPMCs was associated with an increased adhesion of gastric cancer cells to mesothelial cells. These findings suggest that CTGF is not only an important mediator but a potent activator of EMT in peritoneal mesothelial cells, which in turn promotes gastric cancer cell adhesion to peritoneum.

Authors+Show Affiliations

Department of Surgical Oncology, The First Affiliated Hospital of China Medical University, and Department of Cell Biology, China Medical University, Shenyang 110001, Liaoning Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23073116

Citation

Jiang, Cheng-Gang, et al. "Connective Tissue Growth Factor Is a Positive Regulator of Epithelial-mesenchymal Transition and Promotes the Adhesion With Gastric Cancer Cells in Human Peritoneal Mesothelial Cells." Cytokine, vol. 61, no. 1, 2013, pp. 173-80.
Jiang CG, Lv L, Liu FR, et al. Connective tissue growth factor is a positive regulator of epithelial-mesenchymal transition and promotes the adhesion with gastric cancer cells in human peritoneal mesothelial cells. Cytokine. 2013;61(1):173-80.
Jiang, C. G., Lv, L., Liu, F. R., Wang, Z. N., Na, D., Li, F., Li, J. B., Sun, Z., & Xu, H. M. (2013). Connective tissue growth factor is a positive regulator of epithelial-mesenchymal transition and promotes the adhesion with gastric cancer cells in human peritoneal mesothelial cells. Cytokine, 61(1), 173-80. https://doi.org/10.1016/j.cyto.2012.09.013
Jiang CG, et al. Connective Tissue Growth Factor Is a Positive Regulator of Epithelial-mesenchymal Transition and Promotes the Adhesion With Gastric Cancer Cells in Human Peritoneal Mesothelial Cells. Cytokine. 2013;61(1):173-80. PubMed PMID: 23073116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Connective tissue growth factor is a positive regulator of epithelial-mesenchymal transition and promotes the adhesion with gastric cancer cells in human peritoneal mesothelial cells. AU - Jiang,Cheng-Gang, AU - Lv,Ling, AU - Liu,Fu-Rong, AU - Wang,Zhen-Ning, AU - Na,Di, AU - Li,Feng, AU - Li,Jia-Bin, AU - Sun,Zhe, AU - Xu,Hui-Mian, Y1 - 2012/10/13/ PY - 2012/03/06/received PY - 2012/09/02/revised PY - 2012/09/20/accepted PY - 2012/10/18/entrez PY - 2012/10/18/pubmed PY - 2013/6/20/medline SP - 173 EP - 80 JF - Cytokine JO - Cytokine VL - 61 IS - 1 N2 - Connective tissue growth factor (CTGF) is involved in human cancer development and progression. Epithelial to mesenchymal transition (EMT) plays an important role in many biological processes. In this study, we wished to investigate the role of CTGF in EMT of peritoneal mesothelial cells and the effects of CTGF on adhesion of gastric cancer cells to mesothelial cells. Human peritoneal mesothelial cells (HPMCs) were cultured with TGF-β1 or various concentrations of CTGF for different time. The EMT process was monitored by morphology. Real-time RT-PCR and Western blot were used to evaluate the expression of vimentin, α-SMA , E-cadherin and β-catenin. RNA interference was used to achieve selective and specific knockdown of CTGF. We demonstrated that CTGF induced EMT of mesothelial cells in a dose- and time-dependent manner. HPMCs were exposed to TGF-β1 also underwent EMT which was associated with the induction of CTGF expression. Transfection with CTGF siRNA was able to reverse the EMT partially after treatment of TGF-β1. Moreover, the induced EMT of HPMCs was associated with an increased adhesion of gastric cancer cells to mesothelial cells. These findings suggest that CTGF is not only an important mediator but a potent activator of EMT in peritoneal mesothelial cells, which in turn promotes gastric cancer cell adhesion to peritoneum. SN - 1096-0023 UR - https://www.unboundmedicine.com/medline/citation/23073116/Connective_tissue_growth_factor_is_a_positive_regulator_of_epithelial_mesenchymal_transition_and_promotes_the_adhesion_with_gastric_cancer_cells_in_human_peritoneal_mesothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1043-4666(12)00705-3 DB - PRIME DP - Unbound Medicine ER -