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Gypsy, RTE and Mariner transposable elements populate Eyprepocnemis plorans genome.
Genetica. 2012 Sep; 140(7-9):365-74.G

Abstract

We analyze here the presence and abundance of three types of transposable elements (TEs), i.e. Gypsy, RTE and Mariner, in the genome of the grasshopper Eyprepocnemis plorans. PCR experiments allowed amplification, cloning and sequencing of these elements (EploGypI, EploRTE5, EploMar20) from the E. plorans genome. Fluorescent in situ hybridization (FISH) showed that all three elements are restricted to euchromatic regions, thus being absent from the pericentromeric region of all A chromosomes, which contain a satellite DNA (satDNA) and ribosomal DNA (rDNA), and being very scarce in B chromosomes mostly made up of these two types of repetitive DNA. FISH suggested that EploGypI is the most abundant and EploMar20 is the least abundant, with EploRTE5 showing intermediate abundance. An estimation of copy number, by means of quantitative PCR, showed that EploGypI is, by far, the most abundant element, followed by EploRTE5 and EploMar20, in consistency with FISH results. RNA isolation and PCR experiments on complementary DNA (cDNA) showed the presence of transcripts for the three TE elements. The implications of the preferential location of these TE elements into euchromatin, the significance of TE abundance in the giant genome of this species, and a possible relationship between TEs and B chromosome mutability, are discussed.

Authors+Show Affiliations

Departamento de Genética, Universidad de Granada, 18071 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23073915

Citation

Montiel, Eugenia E., et al. "Gypsy, RTE and Mariner Transposable Elements Populate Eyprepocnemis Plorans Genome." Genetica, vol. 140, no. 7-9, 2012, pp. 365-74.
Montiel EE, Cabrero J, Camacho JP, et al. Gypsy, RTE and Mariner transposable elements populate Eyprepocnemis plorans genome. Genetica. 2012;140(7-9):365-74.
Montiel, E. E., Cabrero, J., Camacho, J. P., & López-León, M. D. (2012). Gypsy, RTE and Mariner transposable elements populate Eyprepocnemis plorans genome. Genetica, 140(7-9), 365-74. https://doi.org/10.1007/s10709-012-9686-1
Montiel EE, et al. Gypsy, RTE and Mariner Transposable Elements Populate Eyprepocnemis Plorans Genome. Genetica. 2012;140(7-9):365-74. PubMed PMID: 23073915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gypsy, RTE and Mariner transposable elements populate Eyprepocnemis plorans genome. AU - Montiel,Eugenia E, AU - Cabrero,Josefa, AU - Camacho,Juan Pedro M, AU - López-León,M Dolores, Y1 - 2012/10/17/ PY - 2012/05/05/received PY - 2012/10/08/accepted PY - 2012/10/18/entrez PY - 2012/10/18/pubmed PY - 2013/4/18/medline SP - 365 EP - 74 JF - Genetica JO - Genetica VL - 140 IS - 7-9 N2 - We analyze here the presence and abundance of three types of transposable elements (TEs), i.e. Gypsy, RTE and Mariner, in the genome of the grasshopper Eyprepocnemis plorans. PCR experiments allowed amplification, cloning and sequencing of these elements (EploGypI, EploRTE5, EploMar20) from the E. plorans genome. Fluorescent in situ hybridization (FISH) showed that all three elements are restricted to euchromatic regions, thus being absent from the pericentromeric region of all A chromosomes, which contain a satellite DNA (satDNA) and ribosomal DNA (rDNA), and being very scarce in B chromosomes mostly made up of these two types of repetitive DNA. FISH suggested that EploGypI is the most abundant and EploMar20 is the least abundant, with EploRTE5 showing intermediate abundance. An estimation of copy number, by means of quantitative PCR, showed that EploGypI is, by far, the most abundant element, followed by EploRTE5 and EploMar20, in consistency with FISH results. RNA isolation and PCR experiments on complementary DNA (cDNA) showed the presence of transcripts for the three TE elements. The implications of the preferential location of these TE elements into euchromatin, the significance of TE abundance in the giant genome of this species, and a possible relationship between TEs and B chromosome mutability, are discussed. SN - 1573-6857 UR - https://www.unboundmedicine.com/medline/citation/23073915/Gypsy_RTE_and_Mariner_transposable_elements_populate_Eyprepocnemis_plorans_genome_ L2 - https://doi.org/10.1007/s10709-012-9686-1 DB - PRIME DP - Unbound Medicine ER -