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virG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein.
J Bacteriol 1990; 172(3):1241-9JB

Abstract

The Agrobacterium tumefaciens Ti plasmid virG locus, in conjunction with virA and acetosyringone, activates transcription of the virulence (vir) genes. Insertional and deoxyoligonucleotide-directed mutagenesis studies showed that both octopine and nopaline Ti plasmid virG genes initiate translation at a UUG codon. VirG protein initiated at this UUG codon was found to be 241 amino acid residues in length and had an apparent molecular mass of 27.1 kilodaltons. A Salmonella typhimurium trp-virG transcriptional fusion was constructed to overproduce VirG. Agrobacterium cells containing this gene fusion showed a large increase in virG activity in the presence of virA and acetosyringone. Since the trp promoter is not under virA-virG control, this result indicates that modification of VirG is necessary for its full activity. VirG overproduced in Escherichia coli was purified from inclusion bodies. It was found to be a DNA-binding protein that preferentially bound DNA fragments containing the 5' nontranscribed regions of the virA, -B, -C, -D, and -G operons. Significant specific binding to the 5' nontranscribed region sequences of virE was not detected. DNase I footprinting of the upstream regions of virC-virD and virG showed that VirG binds to sequences around the vir box region.

Authors+Show Affiliations

Department of Biochemistry, University of Minnesota, St. Paul 55108.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2307647

Citation

Pazour, G J., and A Das. "VirG, an Agrobacterium Tumefaciens Transcriptional Activator, Initiates Translation at a UUG Codon and Is a Sequence-specific DNA-binding Protein." Journal of Bacteriology, vol. 172, no. 3, 1990, pp. 1241-9.
Pazour GJ, Das A. VirG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein. J Bacteriol. 1990;172(3):1241-9.
Pazour, G. J., & Das, A. (1990). VirG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein. Journal of Bacteriology, 172(3), pp. 1241-9.
Pazour GJ, Das A. VirG, an Agrobacterium Tumefaciens Transcriptional Activator, Initiates Translation at a UUG Codon and Is a Sequence-specific DNA-binding Protein. J Bacteriol. 1990;172(3):1241-9. PubMed PMID: 2307647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - virG, an Agrobacterium tumefaciens transcriptional activator, initiates translation at a UUG codon and is a sequence-specific DNA-binding protein. AU - Pazour,G J, AU - Das,A, PY - 1990/3/1/pubmed PY - 1990/3/1/medline PY - 1990/3/1/entrez SP - 1241 EP - 9 JF - Journal of bacteriology JO - J. Bacteriol. VL - 172 IS - 3 N2 - The Agrobacterium tumefaciens Ti plasmid virG locus, in conjunction with virA and acetosyringone, activates transcription of the virulence (vir) genes. Insertional and deoxyoligonucleotide-directed mutagenesis studies showed that both octopine and nopaline Ti plasmid virG genes initiate translation at a UUG codon. VirG protein initiated at this UUG codon was found to be 241 amino acid residues in length and had an apparent molecular mass of 27.1 kilodaltons. A Salmonella typhimurium trp-virG transcriptional fusion was constructed to overproduce VirG. Agrobacterium cells containing this gene fusion showed a large increase in virG activity in the presence of virA and acetosyringone. Since the trp promoter is not under virA-virG control, this result indicates that modification of VirG is necessary for its full activity. VirG overproduced in Escherichia coli was purified from inclusion bodies. It was found to be a DNA-binding protein that preferentially bound DNA fragments containing the 5' nontranscribed regions of the virA, -B, -C, -D, and -G operons. Significant specific binding to the 5' nontranscribed region sequences of virE was not detected. DNase I footprinting of the upstream regions of virC-virD and virG showed that VirG binds to sequences around the vir box region. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2307647/virG_an_Agrobacterium_tumefaciens_transcriptional_activator_initiates_translation_at_a_UUG_codon_and_is_a_sequence_specific_DNA_binding_protein_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=2307647 DB - PRIME DP - Unbound Medicine ER -