Tags

Type your tag names separated by a space and hit enter

Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae.
New Phytol. 2012 Dec; 196(4):1197-1207.NP

Abstract

Genomes of the rice (Oryza sativa) xylem and mesophyll pathogens Xanthomonas oryzae pv. oryzae (Xoo) and pv. oryzicola (Xoc) encode numerous secreted transcription factors called transcription activator-like (TAL) effectors. In a few studied rice varieties, some of these contribute to virulence by activating corresponding host susceptibility genes. Some activate disease resistance genes. The roles of X. oryzae TAL effectors in diverse rice backgrounds, however, are poorly understood. Xoo TAL effectors that promote infection by activating SWEET sucrose transporter genes were expressed in TAL effector-deficient X. oryzae strain X11-5A, and assessed in 21 rice varieties. Some were also tested in Xoc on variety Nipponbare. Several Xoc TAL effectors were tested in X11-5A on four rice varieties. Xoo TAL effectors enhanced X11-5A virulence on most varieties, but to varying extents depending on the effector and variety. SWEET genes were activated in all tested varieties, but increased virulence did not correlate with activation level. SWEET activators also enhanced Xoc virulence on Nipponbare. Xoc TAL effectors did not alter X11-5A virulence. SWEET-targeting TAL effectors contribute broadly and non-tissue-specifically to virulence in rice, and their function is affected by host differences besides target sequences. Further, the utility of X11-5A for characterizing individual TAL effectors in rice was established.

Authors+Show Affiliations

Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA. Institut de Recherche pour le Développement, UMR Résistance des Plantes aux Bioagresseurs, IRD-CIRAD-UM2, 911 Avenue Agropolis BP 64501, 34394, Montpellier Cedex 5, France.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, 14850, USA.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA.Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA, 50011, USA. Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, 14850, USA.Department of Bioagricultural Sciences and Pest Management and Program in Plant Molecular Biology, Colorado State University, Fort Collins, CO, 80523-1177, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23078195

Citation

Verdier, Valérie, et al. "Transcription Activator-like (TAL) Effectors Targeting OsSWEET Genes Enhance Virulence On Diverse Rice (Oryza Sativa) Varieties when Expressed Individually in a TAL Effector-deficient Strain of Xanthomonas Oryzae." The New Phytologist, vol. 196, no. 4, 2012, pp. 1197-1207.
Verdier V, Triplett LR, Hummel AW, et al. Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae. New Phytol. 2012;196(4):1197-1207.
Verdier, V., Triplett, L. R., Hummel, A. W., Corral, R., Cernadas, R. A., Schmidt, C. L., Bogdanove, A. J., & Leach, J. E. (2012). Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae. The New Phytologist, 196(4), 1197-1207. https://doi.org/10.1111/j.1469-8137.2012.04367.x
Verdier V, et al. Transcription Activator-like (TAL) Effectors Targeting OsSWEET Genes Enhance Virulence On Diverse Rice (Oryza Sativa) Varieties when Expressed Individually in a TAL Effector-deficient Strain of Xanthomonas Oryzae. New Phytol. 2012;196(4):1197-1207. PubMed PMID: 23078195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcription activator-like (TAL) effectors targeting OsSWEET genes enhance virulence on diverse rice (Oryza sativa) varieties when expressed individually in a TAL effector-deficient strain of Xanthomonas oryzae. AU - Verdier,Valérie, AU - Triplett,Lindsay R, AU - Hummel,Aaron W, AU - Corral,Rene, AU - Cernadas,R Andres, AU - Schmidt,Clarice L, AU - Bogdanove,Adam J, AU - Leach,Jan E, Y1 - 2012/10/18/ PY - 2012/05/03/received PY - 2012/08/31/accepted PY - 2012/10/20/entrez PY - 2012/10/20/pubmed PY - 2013/4/26/medline SP - 1197 EP - 1207 JF - The New phytologist JO - New Phytol VL - 196 IS - 4 N2 - Genomes of the rice (Oryza sativa) xylem and mesophyll pathogens Xanthomonas oryzae pv. oryzae (Xoo) and pv. oryzicola (Xoc) encode numerous secreted transcription factors called transcription activator-like (TAL) effectors. In a few studied rice varieties, some of these contribute to virulence by activating corresponding host susceptibility genes. Some activate disease resistance genes. The roles of X. oryzae TAL effectors in diverse rice backgrounds, however, are poorly understood. Xoo TAL effectors that promote infection by activating SWEET sucrose transporter genes were expressed in TAL effector-deficient X. oryzae strain X11-5A, and assessed in 21 rice varieties. Some were also tested in Xoc on variety Nipponbare. Several Xoc TAL effectors were tested in X11-5A on four rice varieties. Xoo TAL effectors enhanced X11-5A virulence on most varieties, but to varying extents depending on the effector and variety. SWEET genes were activated in all tested varieties, but increased virulence did not correlate with activation level. SWEET activators also enhanced Xoc virulence on Nipponbare. Xoc TAL effectors did not alter X11-5A virulence. SWEET-targeting TAL effectors contribute broadly and non-tissue-specifically to virulence in rice, and their function is affected by host differences besides target sequences. Further, the utility of X11-5A for characterizing individual TAL effectors in rice was established. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/23078195/Transcription_activator_like__TAL__effectors_targeting_OsSWEET_genes_enhance_virulence_on_diverse_rice__Oryza_sativa__varieties_when_expressed_individually_in_a_TAL_effector_deficient_strain_of_Xanthomonas_oryzae_ L2 - https://doi.org/10.1111/j.1469-8137.2012.04367.x DB - PRIME DP - Unbound Medicine ER -