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Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix.
J Nat Med. 2013 Jul; 67(3):657-61.JN

Abstract

A quantitative (1)H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45%. We demonstrated that this method is useful for quantitative analysis of crude drugs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Tanaka R
Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
Yamazaki M
No affiliation info available
Hasada K
No affiliation info available
Nagatsu A
No affiliation info available

MeSH

BenzoatesBridged-Ring CompoundsCalibrationGlucosidesMagnetic Resonance SpectroscopyMonoterpenesPaeoniaPlant RootsPlants, Medicinal

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

23081682

Citation

Tanaka, Rie, et al. "Application of Quantitative 1H-NMR Method to Determination of Paeoniflorin in Paeoniae Radix." Journal of Natural Medicines, vol. 67, no. 3, 2013, pp. 657-61.
Tanaka R, Yamazaki M, Hasada K, et al. Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix. J Nat Med. 2013;67(3):657-61.
Tanaka, R., Yamazaki, M., Hasada, K., & Nagatsu, A. (2013). Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix. Journal of Natural Medicines, 67(3), 657-61. https://doi.org/10.1007/s11418-012-0711-6
Tanaka R, et al. Application of Quantitative 1H-NMR Method to Determination of Paeoniflorin in Paeoniae Radix. J Nat Med. 2013;67(3):657-61. PubMed PMID: 23081682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of quantitative 1H-NMR method to determination of paeoniflorin in Paeoniae radix. AU - Tanaka,Rie, AU - Yamazaki,Marina, AU - Hasada,Keiko, AU - Nagatsu,Akito, Y1 - 2012/10/19/ PY - 2012/07/19/received PY - 2012/09/27/accepted PY - 2012/10/20/entrez PY - 2012/10/20/pubmed PY - 2014/5/23/medline SP - 657 EP - 61 JF - Journal of natural medicines JO - J Nat Med VL - 67 IS - 3 N2 - A quantitative (1)H-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at δ 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45%. We demonstrated that this method is useful for quantitative analysis of crude drugs. SN - 1861-0293 UR - https://www.unboundmedicine.com/medline/citation/23081682/Application_of_quantitative_1H_NMR_method_to_determination_of_paeoniflorin_in_Paeoniae_radix_ L2 - https://dx.doi.org/10.1007/s11418-012-0711-6 DB - PRIME DP - Unbound Medicine ER -