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Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody.
Appl Microbiol Biotechnol. 2013 Feb; 97(4):1553-60.AM

Abstract

Dengue virus is a major international public health concern, and there is a lack of available effective vaccines. Virus-specific epitopes could help in developing epitope peptide vaccine. Previously, a neutralizing monoclonal antibody (mAb) 4F5 against nonstructural protein 3 (NS3) of dengue virus 2 (DV2) was developed in our lab. In this work, the B cell epitope recognized by mAb 4F5 was identified using the phage-displayed peptide library. The results of the binding assay and competitive inhibition assay indicated that the peptides, residues 460-469 (U460-469 RVGRNPKNEN) of DV2 NS3 protein, were the B cell epitopes recognized by mAb 4F5. Furthermore, the epitope peptides and a control peptide were synthesized and then immunized female BALB/c mice. ELISA analysis showed that immunization with synthesized epitope peptide elicited a high level of antibody in mice, and immunofluorescent staining showed that the antisera from fusion epitope-immunized mice also responded to DV2 NS3 protein, which further characterized the specific response of the present epitope peptide. Therefore, the present work revealed the specificity of the newly identified epitope (U460-469) of DV2 NS3 protein, which may shed light on dengue virus (DV) vaccine design, DV pathogenesis study, and even DV diagnostic reagent development.

Authors+Show Affiliations

Department of Histology and Embryology, School of Basic Medicine, Third Military Medical University, Chongqing 400038, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23081772

Citation

Tian, Yanping, et al. "Identification of B Cell Epitopes of Dengue Virus 2 NS3 Protein By Monoclonal Antibody." Applied Microbiology and Biotechnology, vol. 97, no. 4, 2013, pp. 1553-60.
Tian Y, Chen W, Yang Y, et al. Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody. Appl Microbiol Biotechnol. 2013;97(4):1553-60.
Tian, Y., Chen, W., Yang, Y., Xu, X., Zhang, J., Wang, J., Xiao, L., & Chen, Z. (2013). Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody. Applied Microbiology and Biotechnology, 97(4), 1553-60. https://doi.org/10.1007/s00253-012-4419-z
Tian Y, et al. Identification of B Cell Epitopes of Dengue Virus 2 NS3 Protein By Monoclonal Antibody. Appl Microbiol Biotechnol. 2013;97(4):1553-60. PubMed PMID: 23081772.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of B cell epitopes of dengue virus 2 NS3 protein by monoclonal antibody. AU - Tian,Yanping, AU - Chen,Wei, AU - Yang,Ya, AU - Xu,Xiaofeng, AU - Zhang,Junlei, AU - Wang,Jiali, AU - Xiao,Lan, AU - Chen,Zongtao, Y1 - 2012/10/19/ PY - 2012/06/25/received PY - 2012/09/04/accepted PY - 2012/08/28/revised PY - 2012/10/20/entrez PY - 2012/10/20/pubmed PY - 2013/7/11/medline SP - 1553 EP - 60 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 97 IS - 4 N2 - Dengue virus is a major international public health concern, and there is a lack of available effective vaccines. Virus-specific epitopes could help in developing epitope peptide vaccine. Previously, a neutralizing monoclonal antibody (mAb) 4F5 against nonstructural protein 3 (NS3) of dengue virus 2 (DV2) was developed in our lab. In this work, the B cell epitope recognized by mAb 4F5 was identified using the phage-displayed peptide library. The results of the binding assay and competitive inhibition assay indicated that the peptides, residues 460-469 (U460-469 RVGRNPKNEN) of DV2 NS3 protein, were the B cell epitopes recognized by mAb 4F5. Furthermore, the epitope peptides and a control peptide were synthesized and then immunized female BALB/c mice. ELISA analysis showed that immunization with synthesized epitope peptide elicited a high level of antibody in mice, and immunofluorescent staining showed that the antisera from fusion epitope-immunized mice also responded to DV2 NS3 protein, which further characterized the specific response of the present epitope peptide. Therefore, the present work revealed the specificity of the newly identified epitope (U460-469) of DV2 NS3 protein, which may shed light on dengue virus (DV) vaccine design, DV pathogenesis study, and even DV diagnostic reagent development. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/23081772/Identification_of_B_cell_epitopes_of_dengue_virus_2_NS3_protein_by_monoclonal_antibody_ L2 - https://dx.doi.org/10.1007/s00253-012-4419-z DB - PRIME DP - Unbound Medicine ER -