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Ameliorative effect of Vernonia cinerea in vincristine-induced painful neuropathy in rats.
Toxicol Ind Health. 2014 Oct; 30(9):794-805.TI

Abstract

The present study was designed to investigate the antinociceptive potential of Vernonia cinerea (VC) on vincristine-induced painful neuropathy in rats. A chemotherapeutic agent, vincristine (50 μg/kg intraperitoneally for 10 consecutive days), was administered for the induction of neuropathic pain in rats. The painful behavioral changes were assessed using hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests to assess the degree of hyperalgesic and allodynic pain sensation in paw and tail. Tissue biomarker changes including thiobarbituric acid reactive substances (TBARSs), reduced glutathione (GSH) and total calcium levels were estimated in sciatic nerve tissue samples to assess the degree of oxidative stress. Histopathological changes were also observed in transverse sections of rat sciatic nerve tissue. Ethanolic extract of VC leaves and pregabalin were administered for 14 consecutive days from day 0 (day of surgery). Pregabalin served as a positive control in the present study. Vincristine administration resulted in a significant reduction in painful behavioral changes along with a rise in the levels of TBARS, total calcium and decrease in GSH levels when compared with the normal control group. Furthermore, significant histopathological changes were also observed. Pretreatment with VC significantly attenuated vincristine-induced development of painful behavioral, biochemical and histological changes in a dose-dependent manner, which is similar to that of pregabalin-pretreated group. The attenuating effect of VC in vincristine-induced nociceptive painful sensation may be due to its potential of antioxidative, neuroprotective and calcium channel inhibitory action.

Authors+Show Affiliations

School of Chemical and Biotechnology, Sastra University, Thanjavur, Tamil Nadu, India Department of Pharmacognosy, College of Pharmacy, Madurai Medical College, Madurai, Tamil Nadu, India vrkmmcmadurai@gmail.com.Department of Pharmaceutical Sciences and Research, Sankaralingam Bhuvaneshwari College of Pharmacy, Thiruthangal, Sivakasi, Tamil Nadu, India.Center for Nanotechnology and Advanced Biomaterials, School of Chemical and Biotechnology, Sastra University, Thanjavur, Tamil Nadu, India.Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23081859

Citation

Thiagarajan, Venkata Rathina Kumar, et al. "Ameliorative Effect of Vernonia Cinerea in Vincristine-induced Painful Neuropathy in Rats." Toxicology and Industrial Health, vol. 30, no. 9, 2014, pp. 794-805.
Thiagarajan VR, Shanmugam P, Krishnan UM, et al. Ameliorative effect of Vernonia cinerea in vincristine-induced painful neuropathy in rats. Toxicol Ind Health. 2014;30(9):794-805.
Thiagarajan, V. R., Shanmugam, P., Krishnan, U. M., & Muthuraman, A. (2014). Ameliorative effect of Vernonia cinerea in vincristine-induced painful neuropathy in rats. Toxicology and Industrial Health, 30(9), 794-805. https://doi.org/10.1177/0748233712463779
Thiagarajan VR, et al. Ameliorative Effect of Vernonia Cinerea in Vincristine-induced Painful Neuropathy in Rats. Toxicol Ind Health. 2014;30(9):794-805. PubMed PMID: 23081859.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effect of Vernonia cinerea in vincristine-induced painful neuropathy in rats. AU - Thiagarajan,Venkata Rathina Kumar, AU - Shanmugam,Palanichamy, AU - Krishnan,Uma Maheswari, AU - Muthuraman,Arunachalam, Y1 - 2012/10/18/ PY - 2012/10/20/entrez PY - 2012/10/20/pubmed PY - 2015/5/28/medline KW - Calcium KW - Vernonia cinerea KW - neuropathic pain KW - oxidative stress KW - pregabalin KW - vincristine SP - 794 EP - 805 JF - Toxicology and industrial health JO - Toxicol Ind Health VL - 30 IS - 9 N2 - The present study was designed to investigate the antinociceptive potential of Vernonia cinerea (VC) on vincristine-induced painful neuropathy in rats. A chemotherapeutic agent, vincristine (50 μg/kg intraperitoneally for 10 consecutive days), was administered for the induction of neuropathic pain in rats. The painful behavioral changes were assessed using hot plate, acetone drop, paw pressure, Von Frey hair and tail immersion tests to assess the degree of hyperalgesic and allodynic pain sensation in paw and tail. Tissue biomarker changes including thiobarbituric acid reactive substances (TBARSs), reduced glutathione (GSH) and total calcium levels were estimated in sciatic nerve tissue samples to assess the degree of oxidative stress. Histopathological changes were also observed in transverse sections of rat sciatic nerve tissue. Ethanolic extract of VC leaves and pregabalin were administered for 14 consecutive days from day 0 (day of surgery). Pregabalin served as a positive control in the present study. Vincristine administration resulted in a significant reduction in painful behavioral changes along with a rise in the levels of TBARS, total calcium and decrease in GSH levels when compared with the normal control group. Furthermore, significant histopathological changes were also observed. Pretreatment with VC significantly attenuated vincristine-induced development of painful behavioral, biochemical and histological changes in a dose-dependent manner, which is similar to that of pregabalin-pretreated group. The attenuating effect of VC in vincristine-induced nociceptive painful sensation may be due to its potential of antioxidative, neuroprotective and calcium channel inhibitory action. SN - 1477-0393 UR - https://www.unboundmedicine.com/medline/citation/23081859/Ameliorative_effect_of_Vernonia_cinerea_in_vincristine_induced_painful_neuropathy_in_rats_ L2 - https://journals.sagepub.com/doi/10.1177/0748233712463779?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -