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Electric fields guide migration of epidermal stem cells and promote skin wound healing.
Wound Repair Regen. 2012 Nov-Dec; 20(6):840-51.WR

Abstract

Migration of epidermal stem cells (EpSCs) into wounds may play an important role in wound healing. Endogenous electric fields (EFs) arise naturally at wounds. Consistent with previous reports, we measured outward electric currents at rat skin wounds using vibrating probes. Topical use of prostaglandin E2 significantly promoted wound healing. However, it is not known whether EpSCs respond to EFs. We first isolated and characterized EpSCs from rat skin. We then demonstrated that EpSCs isolated from the epidermis migrated directionally toward the cathode in EFs of 50-400 mV/mm. The directedness values increased in a dose- and time-dependent fashion. The migration speed of EpSCs was significantly increased in EFs. EFs induced asymmetric polymerization of intracellular F-actin and activation of the extracellular signal-regulated kinase 1/2 and phosphatidylinositol-3-kinase (PI3K)/protein kinase B pathways. Inhibition of epidermal growth factor receptor, extracellular signal-regulated kinase 1/2, or PI3K significantly inhibited the cathodal distribution of F-actin and the electrotactic response of EpSCs. These data for the first time show that EpSCs possess obvious electrotaxis, in which the epidermal growth factor receptor-mitogen activated protein kinase-PI3K pathways are involved. These data thus suggest a novel aspect of electric signaling in wound healing-to stimulate and guide migration of EpSCs and to regulate wound healing.

Authors+Show Affiliations

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23082865

Citation

Li, Li, et al. "Electric Fields Guide Migration of Epidermal Stem Cells and Promote Skin Wound Healing." Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, vol. 20, no. 6, 2012, pp. 840-51.
Li L, Gu W, Du J, et al. Electric fields guide migration of epidermal stem cells and promote skin wound healing. Wound Repair Regen. 2012;20(6):840-51.
Li, L., Gu, W., Du, J., Reid, B., Deng, X., Liu, Z., Zong, Z., Wang, H., Yao, B., Yang, C., Yan, J., Zeng, L., Chalmers, L., Zhao, M., & Jiang, J. (2012). Electric fields guide migration of epidermal stem cells and promote skin wound healing. Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society, 20(6), 840-51. https://doi.org/10.1111/j.1524-475X.2012.00829.x
Li L, et al. Electric Fields Guide Migration of Epidermal Stem Cells and Promote Skin Wound Healing. Wound Repair Regen. 2012 Nov-Dec;20(6):840-51. PubMed PMID: 23082865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electric fields guide migration of epidermal stem cells and promote skin wound healing. AU - Li,Li, AU - Gu,Wei, AU - Du,Juan, AU - Reid,Brian, AU - Deng,Xianjian, AU - Liu,Zhidai, AU - Zong,Zhaowen, AU - Wang,Haiyan, AU - Yao,Bo, AU - Yang,Ce, AU - Yan,Jun, AU - Zeng,Ling, AU - Chalmers,Laura, AU - Zhao,Min, AU - Jiang,Jianxin, Y1 - 2012/10/19/ PY - 2012/02/14/received PY - 2012/06/06/accepted PY - 2012/10/23/entrez PY - 2012/10/23/pubmed PY - 2013/4/23/medline SP - 840 EP - 51 JF - Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society JO - Wound Repair Regen VL - 20 IS - 6 N2 - Migration of epidermal stem cells (EpSCs) into wounds may play an important role in wound healing. Endogenous electric fields (EFs) arise naturally at wounds. Consistent with previous reports, we measured outward electric currents at rat skin wounds using vibrating probes. Topical use of prostaglandin E2 significantly promoted wound healing. However, it is not known whether EpSCs respond to EFs. We first isolated and characterized EpSCs from rat skin. We then demonstrated that EpSCs isolated from the epidermis migrated directionally toward the cathode in EFs of 50-400 mV/mm. The directedness values increased in a dose- and time-dependent fashion. The migration speed of EpSCs was significantly increased in EFs. EFs induced asymmetric polymerization of intracellular F-actin and activation of the extracellular signal-regulated kinase 1/2 and phosphatidylinositol-3-kinase (PI3K)/protein kinase B pathways. Inhibition of epidermal growth factor receptor, extracellular signal-regulated kinase 1/2, or PI3K significantly inhibited the cathodal distribution of F-actin and the electrotactic response of EpSCs. These data for the first time show that EpSCs possess obvious electrotaxis, in which the epidermal growth factor receptor-mitogen activated protein kinase-PI3K pathways are involved. These data thus suggest a novel aspect of electric signaling in wound healing-to stimulate and guide migration of EpSCs and to regulate wound healing. SN - 1524-475X UR - https://www.unboundmedicine.com/medline/citation/23082865/Electric_fields_guide_migration_of_epidermal_stem_cells_and_promote_skin_wound_healing_ L2 - https://doi.org/10.1111/j.1524-475X.2012.00829.x DB - PRIME DP - Unbound Medicine ER -