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Activation and desensitization of TRPV1 channels in sensory neurons by the PPARα agonist palmitoylethanolamide.
Br J Pharmacol. 2013 Mar; 168(6):1430-44.BJ

Abstract

BACKGROUND AND PURPOSE

Palmitoylethanolamide (PEA) is an endogenous fatty acid amide displaying anti-inflammatory and analgesic actions. To investigate the molecular mechanism responsible for these effects, the ability of PEA and of pain-inducing stimuli such as capsaicin (CAP) or bradykinin (BK) to influence intracellular calcium concentrations ([Ca²⁺](i)) in peripheral sensory neurons, has been assessed in the present study. The potential involvement of the transcription factor PPARα and of TRPV1 channels in PEA-induced effects was also studied.

EXPERIMENTAL APPROACH

[Ca²⁺](i) was evaluated by single-cell microfluorimetry in differentiated F11 cells. Activation of TRPV1 channels was assessed by imaging and patch-clamp techniques in CHO cells transiently-transfected with rat TRPV1 cDNA.

KEY RESULTS

In F11 cells, PEA (1-30 μM) dose-dependently increased [Ca²⁺](i). The TRPV1 antagonists capsazepine (1 μM) and SB-366791 (1 μM), as well as the PPARα antagonist GW-6471 (10 μM), inhibited PEA-induced [Ca²⁺](i) increase; blockers of cannabinoid receptors were ineffective. PEA activated TRPV1 channels heterologously expressed in CHO cells; this effect appeared to be mediated at least in part by PPARα. When compared with CAP, PEA showed similar potency and lower efficacy, and caused stronger TRPV1 currents desensitization. Sub-effective PEA concentrations, closer to those found in vivo, counteracted CAP- and BK-induced [Ca²⁺](i) transients, as well as CAP-induced TRPV1 activation.

CONCLUSIONS AND IMPLICATIONS

Activation of PPARα and TRPV1 channels, rather than of cannabinoid receptors, largely mediate PEA-induced [Ca²⁺](i) transients in sensory neurons. Differential TRPV1 activation and desensitization by CAP and PEA might contribute to their distinct pharmacological profile, possibly translating into potentially relevant clinical differences.

Authors+Show Affiliations

Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23083124

Citation

Ambrosino, Paolo, et al. "Activation and Desensitization of TRPV1 Channels in Sensory Neurons By the PPARα Agonist Palmitoylethanolamide." British Journal of Pharmacology, vol. 168, no. 6, 2013, pp. 1430-44.
Ambrosino P, Soldovieri MV, Russo C, et al. Activation and desensitization of TRPV1 channels in sensory neurons by the PPARα agonist palmitoylethanolamide. Br J Pharmacol. 2013;168(6):1430-44.
Ambrosino, P., Soldovieri, M. V., Russo, C., & Taglialatela, M. (2013). Activation and desensitization of TRPV1 channels in sensory neurons by the PPARα agonist palmitoylethanolamide. British Journal of Pharmacology, 168(6), 1430-44. https://doi.org/10.1111/bph.12029
Ambrosino P, et al. Activation and Desensitization of TRPV1 Channels in Sensory Neurons By the PPARα Agonist Palmitoylethanolamide. Br J Pharmacol. 2013;168(6):1430-44. PubMed PMID: 23083124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation and desensitization of TRPV1 channels in sensory neurons by the PPARα agonist palmitoylethanolamide. AU - Ambrosino,Paolo, AU - Soldovieri,Maria Virginia, AU - Russo,Claudio, AU - Taglialatela,Maurizio, PY - 2012/05/09/received PY - 2012/10/03/revised PY - 2012/10/10/accepted PY - 2012/10/23/entrez PY - 2012/10/23/pubmed PY - 2013/8/24/medline SP - 1430 EP - 44 JF - British journal of pharmacology JO - Br J Pharmacol VL - 168 IS - 6 N2 - BACKGROUND AND PURPOSE: Palmitoylethanolamide (PEA) is an endogenous fatty acid amide displaying anti-inflammatory and analgesic actions. To investigate the molecular mechanism responsible for these effects, the ability of PEA and of pain-inducing stimuli such as capsaicin (CAP) or bradykinin (BK) to influence intracellular calcium concentrations ([Ca²⁺](i)) in peripheral sensory neurons, has been assessed in the present study. The potential involvement of the transcription factor PPARα and of TRPV1 channels in PEA-induced effects was also studied. EXPERIMENTAL APPROACH: [Ca²⁺](i) was evaluated by single-cell microfluorimetry in differentiated F11 cells. Activation of TRPV1 channels was assessed by imaging and patch-clamp techniques in CHO cells transiently-transfected with rat TRPV1 cDNA. KEY RESULTS: In F11 cells, PEA (1-30 μM) dose-dependently increased [Ca²⁺](i). The TRPV1 antagonists capsazepine (1 μM) and SB-366791 (1 μM), as well as the PPARα antagonist GW-6471 (10 μM), inhibited PEA-induced [Ca²⁺](i) increase; blockers of cannabinoid receptors were ineffective. PEA activated TRPV1 channels heterologously expressed in CHO cells; this effect appeared to be mediated at least in part by PPARα. When compared with CAP, PEA showed similar potency and lower efficacy, and caused stronger TRPV1 currents desensitization. Sub-effective PEA concentrations, closer to those found in vivo, counteracted CAP- and BK-induced [Ca²⁺](i) transients, as well as CAP-induced TRPV1 activation. CONCLUSIONS AND IMPLICATIONS: Activation of PPARα and TRPV1 channels, rather than of cannabinoid receptors, largely mediate PEA-induced [Ca²⁺](i) transients in sensory neurons. Differential TRPV1 activation and desensitization by CAP and PEA might contribute to their distinct pharmacological profile, possibly translating into potentially relevant clinical differences. SN - 1476-5381 UR - https://www.unboundmedicine.com/medline/citation/23083124/Activation_and_desensitization_of_TRPV1_channels_in_sensory_neurons_by_the_PPAR��_agonist_palmitoylethanolamide_ L2 - https://doi.org/10.1111/bph.12029 DB - PRIME DP - Unbound Medicine ER -