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A unique caleosin serving as the major integral protein in oil bodies isolated from Chlorella sp. cells cultured with limited nitrogen.
Plant Physiol Biochem. 2012 Dec; 61:80-7.PP

Abstract

Accumulation of oil bodies was successfully induced in a microalga, Chlorella sp., cultured in a nitrogen-limited medium. The oil bodies were initially assembled as many small entities (mostly 0.1-1 μm), and lately found as a major irregular compartment (>3 μm) occupying more than half of the cell space. Approximately, two thirds of oil bodies isolated from Chlorella cells were broken and formed a transparent oil layer on top of the milky compact layer of the remaining stable oil bodies after being washed with 0.1% triton X-100. The stable oil bodies mainly comprised triacylglycerols as examined by thin layer chromatography analysis and confirmed by both Nile red and BODIPY stainings. Integrity of these stable oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunological cross-recognition revealed that a major protein of 29 kDa, tentatively identified as caleosin, was exclusively present in Chlorella oil bodies. Mass spectrometric analysis showed that the putative caleosin possessed a trypic fragment of 13 residues matching to that of a hypothetical caleosin in Picea sitchensis. With the aid of a degenerate primer designed according to the tryptic peptide, a complete cDNA fragment encoding this putative caleosin was obtained by PCR. Phylogenetic tree analysis supports that Chlorella caleosin is the most primitive caleosin found in oil bodies to date.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23085585

Citation

Lin, I-Ping, et al. "A Unique Caleosin Serving as the Major Integral Protein in Oil Bodies Isolated From Chlorella Sp. Cells Cultured With Limited Nitrogen." Plant Physiology and Biochemistry : PPB, vol. 61, 2012, pp. 80-7.
Lin IP, Jiang PL, Chen CS, et al. A unique caleosin serving as the major integral protein in oil bodies isolated from Chlorella sp. cells cultured with limited nitrogen. Plant Physiol Biochem. 2012;61:80-7.
Lin, I. P., Jiang, P. L., Chen, C. S., & Tzen, J. T. (2012). A unique caleosin serving as the major integral protein in oil bodies isolated from Chlorella sp. cells cultured with limited nitrogen. Plant Physiology and Biochemistry : PPB, 61, 80-7. https://doi.org/10.1016/j.plaphy.2012.09.008
Lin IP, et al. A Unique Caleosin Serving as the Major Integral Protein in Oil Bodies Isolated From Chlorella Sp. Cells Cultured With Limited Nitrogen. Plant Physiol Biochem. 2012;61:80-7. PubMed PMID: 23085585.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A unique caleosin serving as the major integral protein in oil bodies isolated from Chlorella sp. cells cultured with limited nitrogen. AU - Lin,I-Ping, AU - Jiang,Pei-Luen, AU - Chen,Chii-Shiarng, AU - Tzen,Jason T C, Y1 - 2012/09/26/ PY - 2012/08/18/received PY - 2012/09/19/accepted PY - 2012/10/23/entrez PY - 2012/10/23/pubmed PY - 2013/6/1/medline SP - 80 EP - 7 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 61 N2 - Accumulation of oil bodies was successfully induced in a microalga, Chlorella sp., cultured in a nitrogen-limited medium. The oil bodies were initially assembled as many small entities (mostly 0.1-1 μm), and lately found as a major irregular compartment (>3 μm) occupying more than half of the cell space. Approximately, two thirds of oil bodies isolated from Chlorella cells were broken and formed a transparent oil layer on top of the milky compact layer of the remaining stable oil bodies after being washed with 0.1% triton X-100. The stable oil bodies mainly comprised triacylglycerols as examined by thin layer chromatography analysis and confirmed by both Nile red and BODIPY stainings. Integrity of these stable oil bodies was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Immunological cross-recognition revealed that a major protein of 29 kDa, tentatively identified as caleosin, was exclusively present in Chlorella oil bodies. Mass spectrometric analysis showed that the putative caleosin possessed a trypic fragment of 13 residues matching to that of a hypothetical caleosin in Picea sitchensis. With the aid of a degenerate primer designed according to the tryptic peptide, a complete cDNA fragment encoding this putative caleosin was obtained by PCR. Phylogenetic tree analysis supports that Chlorella caleosin is the most primitive caleosin found in oil bodies to date. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/23085585/A_unique_caleosin_serving_as_the_major_integral_protein_in_oil_bodies_isolated_from_Chlorella_sp__cells_cultured_with_limited_nitrogen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(12)00251-3 DB - PRIME DP - Unbound Medicine ER -