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Changes of human decidual natural killer cells cocultured with YFP-Toxoplasma gondii: implications for abnormal pregnancy.
Fertil Steril 2013; 99(2):427-32FS

Abstract

OBJECTIVE

To investigate the changes of human decidual natural killer (dNK) cells cocultured with Toxoplasma gondii in vitro and to infer implications on pregnancy.

DESIGN

Case-control study.

SETTING

College and hospital.

PATIENT(S)

Decidual tissue was obtained from 85 patients undergoing voluntary abortion during the first trimester of gestation (6-12 weeks).

INTERVENTION(S)

None.

MAIN OUTCOME MEASURE(S)

The dNK cells were isolated and infected with YFP-Toxoplasma gondii. Cells were observed by fluorescence and confocal microscopy. The CD56(bright)CD16(-)/CD56(dim)CD16(+) dNK ratio, expression of KIR2DL4, ILT-2, and NKG2D on dNK cells were analyzed by flow cytometry and the cytotoxic activity of infected dNK cells were evaluated.

RESULT(S)

The CD56(dim)CD16(+)/CD56(bright)CD16(-) dNK ratio was significantly elevated at 12, 24, and 48 hours after YFP-T. gondii infection. Expression of KIR2DL4, ILT-2, and NKG2D were increased after infection, but NKG2D were significantly higher than those of KIR2DL4 and ILT-2. Both the CD56(dim)CD16(+)/CD56(bright)CD16(-) dNK ratio and NKG2D expression were correlated with dNK cytotoxic activity.

CONCLUSION(S)

Enhanced dNK cytotoxicity due to increased CD16 and NKG2D expression may contribute to abnormal pregnancy outcomes observed with maternal infection with T. gondii.

Authors+Show Affiliations

Department of Immunology, Binzhou Medical University, Yantai, Shandong, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23089237

Citation

Xu, Xiaoyan, et al. "Changes of Human Decidual Natural Killer Cells Cocultured With YFP-Toxoplasma Gondii: Implications for Abnormal Pregnancy." Fertility and Sterility, vol. 99, no. 2, 2013, pp. 427-32.
Xu X, Fu Q, Zhang Q, et al. Changes of human decidual natural killer cells cocultured with YFP-Toxoplasma gondii: implications for abnormal pregnancy. Fertil Steril. 2013;99(2):427-32.
Xu, X., Fu, Q., Zhang, Q., Zhao, M., Gao, Z., Liu, X., ... Hu, X. (2013). Changes of human decidual natural killer cells cocultured with YFP-Toxoplasma gondii: implications for abnormal pregnancy. Fertility and Sterility, 99(2), pp. 427-32. doi:10.1016/j.fertnstert.2012.09.016.
Xu X, et al. Changes of Human Decidual Natural Killer Cells Cocultured With YFP-Toxoplasma Gondii: Implications for Abnormal Pregnancy. Fertil Steril. 2013;99(2):427-32. PubMed PMID: 23089237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes of human decidual natural killer cells cocultured with YFP-Toxoplasma gondii: implications for abnormal pregnancy. AU - Xu,Xiaoyan, AU - Fu,Qiang, AU - Zhang,Qun, AU - Zhao,Mingdong, AU - Gao,Zonghua, AU - Liu,Xianbing, AU - Liu,Yang, AU - Hu,Xuemei, Y1 - 2012/10/22/ PY - 2012/05/18/received PY - 2012/09/06/revised PY - 2012/09/06/accepted PY - 2012/10/24/entrez PY - 2012/10/24/pubmed PY - 2013/4/5/medline SP - 427 EP - 32 JF - Fertility and sterility JO - Fertil. Steril. VL - 99 IS - 2 N2 - OBJECTIVE: To investigate the changes of human decidual natural killer (dNK) cells cocultured with Toxoplasma gondii in vitro and to infer implications on pregnancy. DESIGN: Case-control study. SETTING: College and hospital. PATIENT(S): Decidual tissue was obtained from 85 patients undergoing voluntary abortion during the first trimester of gestation (6-12 weeks). INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The dNK cells were isolated and infected with YFP-Toxoplasma gondii. Cells were observed by fluorescence and confocal microscopy. The CD56(bright)CD16(-)/CD56(dim)CD16(+) dNK ratio, expression of KIR2DL4, ILT-2, and NKG2D on dNK cells were analyzed by flow cytometry and the cytotoxic activity of infected dNK cells were evaluated. RESULT(S): The CD56(dim)CD16(+)/CD56(bright)CD16(-) dNK ratio was significantly elevated at 12, 24, and 48 hours after YFP-T. gondii infection. Expression of KIR2DL4, ILT-2, and NKG2D were increased after infection, but NKG2D were significantly higher than those of KIR2DL4 and ILT-2. Both the CD56(dim)CD16(+)/CD56(bright)CD16(-) dNK ratio and NKG2D expression were correlated with dNK cytotoxic activity. CONCLUSION(S): Enhanced dNK cytotoxicity due to increased CD16 and NKG2D expression may contribute to abnormal pregnancy outcomes observed with maternal infection with T. gondii. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/23089237/Changes_of_human_decidual_natural_killer_cells_cocultured_with_YFP_Toxoplasma_gondii:_implications_for_abnormal_pregnancy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(12)02235-2 DB - PRIME DP - Unbound Medicine ER -