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Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells.
J Immunol. 2012 Dec 01; 189(11):5402-10.JI

Abstract

Given that TLRs and sphingosine-1-phosphate (S1P) are key players in inflammation, we explored the potential interplay between TLRs and S1P in the adhesion/inflammatory pathways in primary human endothelial cells. As determined by Western blot and flow cytometry, cells treated with LPS (a TLR4 ligand) and S1P showed significantly enhanced expression of adhesion molecules such as ICAM-1 and E-selectin compared with the effect of either ligand alone. Cell-type differences on E-selectin upregulation were observed. In contrast, no cooperation effect on ICAM-1 or E-selectin was observed with a TLR2/TLR1 ligand. Consistent with an increase in adhesion molecule expression, endothelial cell treatment with LPS plus S1P significantly enhanced adhesion of PBMCs under shear stress conditions compared with the effect of either ligand alone and exhibited comparable levels of cell adhesion strength as those after TNF-α treatment. Moreover, LPS and S1P cooperated to increase the expression of proinflammatory molecules such as IL-6, cyclooxygenase-2, and prostacyclin, as determined by ELISA and Western blot. The analysis of signaling pathways revealed the synergistic phosphorylation of ERK upon LPS plus S1P treatment of HUVEC and human aortic endothelial cells and cell-type differences on p38 and NF-κB activation. Moreover, pharmacological and small interfering RNA experiments disclosed the involvement of S1P(1/3) and NF-κB in the cooperation effect and that cell origin determines the S1P receptors and signaling routes involved. Sphingosine kinase activity induction upon LPS plus S1P treatment suggests S1P- Sphingosine kinase axis involvement. In summary, LPS and S1P cooperate to increase proinflammatory molecules in endothelial cells and, in turn, to augment leukocyte adhesion, thus exacerbating S1P-mediated proadhesive/proinflammatory properties.

Authors+Show Affiliations

Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas and Universidad de Valladolid, Valladolid 47003, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23089395

Citation

Fernández-Pisonero, Isabel, et al. "Lipopolysaccharide and Sphingosine-1-phosphate Cooperate to Induce Inflammatory Molecules and Leukocyte Adhesion in Endothelial Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 189, no. 11, 2012, pp. 5402-10.
Fernández-Pisonero I, Dueñas AI, Barreiro O, et al. Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells. J Immunol. 2012;189(11):5402-10.
Fernández-Pisonero, I., Dueñas, A. I., Barreiro, O., Montero, O., Sánchez-Madrid, F., & García-Rodríguez, C. (2012). Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells. Journal of Immunology (Baltimore, Md. : 1950), 189(11), 5402-10. https://doi.org/10.4049/jimmunol.1201309
Fernández-Pisonero I, et al. Lipopolysaccharide and Sphingosine-1-phosphate Cooperate to Induce Inflammatory Molecules and Leukocyte Adhesion in Endothelial Cells. J Immunol. 2012 Dec 1;189(11):5402-10. PubMed PMID: 23089395.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells. AU - Fernández-Pisonero,Isabel, AU - Dueñas,Ana I, AU - Barreiro,Olga, AU - Montero,Olimpio, AU - Sánchez-Madrid,Francisco, AU - García-Rodríguez,Carmen, Y1 - 2012/10/22/ PY - 2012/10/24/entrez PY - 2012/10/24/pubmed PY - 2013/1/19/medline SP - 5402 EP - 10 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 189 IS - 11 N2 - Given that TLRs and sphingosine-1-phosphate (S1P) are key players in inflammation, we explored the potential interplay between TLRs and S1P in the adhesion/inflammatory pathways in primary human endothelial cells. As determined by Western blot and flow cytometry, cells treated with LPS (a TLR4 ligand) and S1P showed significantly enhanced expression of adhesion molecules such as ICAM-1 and E-selectin compared with the effect of either ligand alone. Cell-type differences on E-selectin upregulation were observed. In contrast, no cooperation effect on ICAM-1 or E-selectin was observed with a TLR2/TLR1 ligand. Consistent with an increase in adhesion molecule expression, endothelial cell treatment with LPS plus S1P significantly enhanced adhesion of PBMCs under shear stress conditions compared with the effect of either ligand alone and exhibited comparable levels of cell adhesion strength as those after TNF-α treatment. Moreover, LPS and S1P cooperated to increase the expression of proinflammatory molecules such as IL-6, cyclooxygenase-2, and prostacyclin, as determined by ELISA and Western blot. The analysis of signaling pathways revealed the synergistic phosphorylation of ERK upon LPS plus S1P treatment of HUVEC and human aortic endothelial cells and cell-type differences on p38 and NF-κB activation. Moreover, pharmacological and small interfering RNA experiments disclosed the involvement of S1P(1/3) and NF-κB in the cooperation effect and that cell origin determines the S1P receptors and signaling routes involved. Sphingosine kinase activity induction upon LPS plus S1P treatment suggests S1P- Sphingosine kinase axis involvement. In summary, LPS and S1P cooperate to increase proinflammatory molecules in endothelial cells and, in turn, to augment leukocyte adhesion, thus exacerbating S1P-mediated proadhesive/proinflammatory properties. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/23089395/Lipopolysaccharide_and_sphingosine_1_phosphate_cooperate_to_induce_inflammatory_molecules_and_leukocyte_adhesion_in_endothelial_cells_ DB - PRIME DP - Unbound Medicine ER -