Tags

Type your tag names separated by a space and hit enter

MTHFR gene C677T mutation and ACE gene I/D polymorphism in Turkish patients with osteoarthritis.
Dis Markers 2013; 34(1):17-22DM

Abstract

Osteoarthritis is a degenerative joint disorder resulting in destruction of articular cartilage, osteophyte formation, and subchondral bone sclerosis. In recent years, numerous genetic factors have been identified and implicated in osteoarthritis. The aim of the current study was to examine the influence of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) variations on the risk of osteoarthritis. Genomic DNA is obtained from 421 persons (221 patients with osteoarthritis and 200 healthy controls). ACE gene I/D polymorphism genotypes were determined using polymerase chain reaction using I and D allele-specific primers. The MTHFR C677T mutation was analyzed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) methods. We found significant difference between the groups with respect to both ACE and MTHFR genotype distributions (p< 0.001, p< 0.001 respectively). Our study suggests that ACE gene DD genotype and MTHFR gene CC genotype could be used as genetic markers in osteoarthritis in Turkish study populations.

Authors+Show Affiliations

Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Gaziosmanpaşa University, Tokat, Turkey. ainanir@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23089924

Citation

Inanir, Ahmet, et al. "MTHFR Gene C677T Mutation and ACE Gene I/D Polymorphism in Turkish Patients With Osteoarthritis." Disease Markers, vol. 34, no. 1, 2013, pp. 17-22.
Inanir A, Yigit S, Tural S, et al. MTHFR gene C677T mutation and ACE gene I/D polymorphism in Turkish patients with osteoarthritis. Dis Markers. 2013;34(1):17-22.
Inanir, A., Yigit, S., Tural, S., Cecen, O., & Yildirim, E. (2013). MTHFR gene C677T mutation and ACE gene I/D polymorphism in Turkish patients with osteoarthritis. Disease Markers, 34(1), pp. 17-22. doi:10.3233/DMA-2012-00939.
Inanir A, et al. MTHFR Gene C677T Mutation and ACE Gene I/D Polymorphism in Turkish Patients With Osteoarthritis. Dis Markers. 2013;34(1):17-22. PubMed PMID: 23089924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MTHFR gene C677T mutation and ACE gene I/D polymorphism in Turkish patients with osteoarthritis. AU - Inanir,Ahmet, AU - Yigit,Serbulent, AU - Tural,Sengul, AU - Cecen,Osman, AU - Yildirim,Eren, PY - 2012/10/24/entrez PY - 2012/10/24/pubmed PY - 2013/5/29/medline SP - 17 EP - 22 JF - Disease markers JO - Dis. Markers VL - 34 IS - 1 N2 - Osteoarthritis is a degenerative joint disorder resulting in destruction of articular cartilage, osteophyte formation, and subchondral bone sclerosis. In recent years, numerous genetic factors have been identified and implicated in osteoarthritis. The aim of the current study was to examine the influence of methylenetetrahydrofolate reductase (MTHFR) gene C677T mutation and angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) variations on the risk of osteoarthritis. Genomic DNA is obtained from 421 persons (221 patients with osteoarthritis and 200 healthy controls). ACE gene I/D polymorphism genotypes were determined using polymerase chain reaction using I and D allele-specific primers. The MTHFR C677T mutation was analyzed by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) methods. We found significant difference between the groups with respect to both ACE and MTHFR genotype distributions (p< 0.001, p< 0.001 respectively). Our study suggests that ACE gene DD genotype and MTHFR gene CC genotype could be used as genetic markers in osteoarthritis in Turkish study populations. SN - 1875-8630 UR - https://www.unboundmedicine.com/medline/citation/23089924/MTHFR_gene_C677T_mutation_and_ACE_gene_I/D_polymorphism_in_Turkish_patients_with_osteoarthritis_ L2 - https://dx.doi.org/10.3233/DMA-2012-00939 DB - PRIME DP - Unbound Medicine ER -