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Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea.
Theor Appl Genet. 2013 Feb; 126(2):549-56.TA

Abstract

The lack of resistant source has greatly restrained resistance breeding of rapeseed (Brassica napus, AACC) against Sclerotinia sclerotiorum which causes severe yield losses in rapeseed production all over the world. Recently, several wild Brassica oleracea accessions (CC) with high level of resistance have been identified (Mei et al. in Euphytica 177:393-400, 2011), bringing a new hope to improve Sclerotinia resistance of rapeseed. To map quantitative trait loci (QTL) for Sclerotinia resistance from wild B. oleracea, an F2 population consisting of 149 genotypes, with several clones of each genotypes, was developed from one F1 individual derived from the cross between a resistant accession of wild B. oleracea (B. incana) and a susceptible accession of cultivated B. oleracea var. alboglabra. The F2 population was evaluated for Sclerotinia reaction in 2009 and 2010 under controlled condition. Significant differences among genotypes and high heritability for leaf and stem reaction indicated that genetic components accounted for a large portion of the phenotypic variance. A total of 12 QTL for leaf resistance and six QTL for stem resistance were identified in 2 years, each explaining 2.2-28.4 % of the phenotypic variation. The combined effect of alleles from wild B. oleracea reduced the relative susceptibility by 22.5 % in leaves and 15 % in stems on average over 2 years. A 12.8-cM genetic region on chromosome C09 of B. oleracea consisting of two major QTL intervals for both leaf and stem resistance was assigned into a 2.7-Mb genomic region on chromosome A09 of B. rapa, harboring about 30 putative resistance-related genes. Significant negative corrections were found between flowering time and relative susceptibility of leaf and stem. The association of flowering time with Sclerotinia resistance is discussed.

Authors+Show Affiliations

College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China. jiaqinmay@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23096003

Citation

Mei, Jiaqin, et al. "Identification of Genomic Regions Involved in Resistance Against Sclerotinia Sclerotiorum From Wild Brassica Oleracea." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 126, no. 2, 2013, pp. 549-56.
Mei J, Ding Y, Lu K, et al. Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. Theor Appl Genet. 2013;126(2):549-56.
Mei, J., Ding, Y., Lu, K., Wei, D., Liu, Y., Disi, J. O., Li, J., Liu, L., Liu, S., McKay, J., & Qian, W. (2013). Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 126(2), 549-56. https://doi.org/10.1007/s00122-012-2000-x
Mei J, et al. Identification of Genomic Regions Involved in Resistance Against Sclerotinia Sclerotiorum From Wild Brassica Oleracea. Theor Appl Genet. 2013;126(2):549-56. PubMed PMID: 23096003.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of genomic regions involved in resistance against Sclerotinia sclerotiorum from wild Brassica oleracea. AU - Mei,Jiaqin, AU - Ding,Yijuan, AU - Lu,Kun, AU - Wei,Dayong, AU - Liu,Yao, AU - Disi,Joseph Onwusemu, AU - Li,Jiana, AU - Liu,Liezhao, AU - Liu,Shengyi, AU - McKay,John, AU - Qian,Wei, Y1 - 2012/10/25/ PY - 2012/06/14/received PY - 2012/10/06/accepted PY - 2012/10/26/entrez PY - 2012/10/26/pubmed PY - 2013/7/24/medline SP - 549 EP - 56 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 126 IS - 2 N2 - The lack of resistant source has greatly restrained resistance breeding of rapeseed (Brassica napus, AACC) against Sclerotinia sclerotiorum which causes severe yield losses in rapeseed production all over the world. Recently, several wild Brassica oleracea accessions (CC) with high level of resistance have been identified (Mei et al. in Euphytica 177:393-400, 2011), bringing a new hope to improve Sclerotinia resistance of rapeseed. To map quantitative trait loci (QTL) for Sclerotinia resistance from wild B. oleracea, an F2 population consisting of 149 genotypes, with several clones of each genotypes, was developed from one F1 individual derived from the cross between a resistant accession of wild B. oleracea (B. incana) and a susceptible accession of cultivated B. oleracea var. alboglabra. The F2 population was evaluated for Sclerotinia reaction in 2009 and 2010 under controlled condition. Significant differences among genotypes and high heritability for leaf and stem reaction indicated that genetic components accounted for a large portion of the phenotypic variance. A total of 12 QTL for leaf resistance and six QTL for stem resistance were identified in 2 years, each explaining 2.2-28.4 % of the phenotypic variation. The combined effect of alleles from wild B. oleracea reduced the relative susceptibility by 22.5 % in leaves and 15 % in stems on average over 2 years. A 12.8-cM genetic region on chromosome C09 of B. oleracea consisting of two major QTL intervals for both leaf and stem resistance was assigned into a 2.7-Mb genomic region on chromosome A09 of B. rapa, harboring about 30 putative resistance-related genes. Significant negative corrections were found between flowering time and relative susceptibility of leaf and stem. The association of flowering time with Sclerotinia resistance is discussed. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/23096003/Identification_of_genomic_regions_involved_in_resistance_against_Sclerotinia_sclerotiorum_from_wild_Brassica_oleracea_ L2 - https://dx.doi.org/10.1007/s00122-012-2000-x DB - PRIME DP - Unbound Medicine ER -