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Effects of chronic treatment with corticosterone and imipramine on fos immunoreactivity and adult hippocampal neurogenesis.
Behav Brain Res. 2013 Feb 01; 238:170-7.BB

Abstract

In a previous study we showed that rats chronically treated with corticosterone (CORT) display anxiogenic behavior, evidenced by facilitation of avoidance responses in the elevated T-maze (ETM) model of anxiety. Treatment with the tricyclic antidepressant imipramine significantly reversed the anxiogenic effects of CORT, while inhibiting ETM escape, a response related to panic disorder. To better understand the neurobiological mechanisms underlying these behavioral effects, analysis of c-fos protein immunoreactivity (fos-ir) was used here to map areas activated by chronic CORT (200 mg pellets, 21-day release) and imipramine (15 mg/kg, IP) administration. We also evaluated the number of cells expressing the neurogenesis marker doublecortin (DCX) in the hippocampus and measured plasma CORT levels on the 21st day of treatment. Results showed that CORT increased fos-ir in the ventrolateral septum, medial amygdala and paraventricular hypothalamic nucleus and decreased fos-ir in the lateral periaqueductal gray. Imipramine, on the other hand, increased fos-ir in the medial amygdala and decreased fos-ir in the anterior hypothalamus. CORT also decreased the number of DCX-positive cells in the ventral and dorsal hippocampus, an effect antagonized by imipramine. CORT levels were significantly higher after treatment. These data suggest that the behavioral effects of CORT and imipramine are mediated through specific, at times overlapping, neuronal circuits, which might be of relevance to a better understanding of the physiopathology of generalized anxiety and panic disorder.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Diniz L
Department of Psychiatry, Federal University of São Paulo, São Paulo, SP, Brazil.
dos Santos TB
No affiliation info available
Britto LR
No affiliation info available
Céspedes IC
No affiliation info available
Garcia MC
No affiliation info available
Spadari-Bratfisch RC
No affiliation info available
Medalha CC
No affiliation info available
de Castro GM
No affiliation info available
Montesano FT
No affiliation info available
Viana MB
No affiliation info available

MeSH

AmygdalaAnimalsCorticosteroneDoublecortin Domain ProteinsDoublecortin ProteinHippocampusHypothalamusImipramineMaleMicrotubule-Associated ProteinsNeurogenesisNeuronsNeuropeptidesProto-Oncogene Proteins c-fosRatsRats, Wistar

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23098799

Citation

Diniz, L, et al. "Effects of Chronic Treatment With Corticosterone and Imipramine On Fos Immunoreactivity and Adult Hippocampal Neurogenesis." Behavioural Brain Research, vol. 238, 2013, pp. 170-7.
Diniz L, dos Santos TB, Britto LR, et al. Effects of chronic treatment with corticosterone and imipramine on fos immunoreactivity and adult hippocampal neurogenesis. Behav Brain Res. 2013;238:170-7.
Diniz, L., dos Santos, T. B., Britto, L. R., Céspedes, I. C., Garcia, M. C., Spadari-Bratfisch, R. C., Medalha, C. C., de Castro, G. M., Montesano, F. T., & Viana, M. B. (2013). Effects of chronic treatment with corticosterone and imipramine on fos immunoreactivity and adult hippocampal neurogenesis. Behavioural Brain Research, 238, 170-7. https://doi.org/10.1016/j.bbr.2012.10.024
Diniz L, et al. Effects of Chronic Treatment With Corticosterone and Imipramine On Fos Immunoreactivity and Adult Hippocampal Neurogenesis. Behav Brain Res. 2013 Feb 1;238:170-7. PubMed PMID: 23098799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of chronic treatment with corticosterone and imipramine on fos immunoreactivity and adult hippocampal neurogenesis. AU - Diniz,L, AU - dos Santos,T B, AU - Britto,L R G, AU - Céspedes,I C, AU - Garcia,M C, AU - Spadari-Bratfisch,R C, AU - Medalha,C C, AU - de Castro,G M, AU - Montesano,F T, AU - Viana,M B, Y1 - 2012/10/22/ PY - 2012/06/27/received PY - 2012/10/10/revised PY - 2012/10/14/accepted PY - 2012/10/27/entrez PY - 2012/10/27/pubmed PY - 2013/5/18/medline SP - 170 EP - 7 JF - Behavioural brain research JO - Behav Brain Res VL - 238 N2 - In a previous study we showed that rats chronically treated with corticosterone (CORT) display anxiogenic behavior, evidenced by facilitation of avoidance responses in the elevated T-maze (ETM) model of anxiety. Treatment with the tricyclic antidepressant imipramine significantly reversed the anxiogenic effects of CORT, while inhibiting ETM escape, a response related to panic disorder. To better understand the neurobiological mechanisms underlying these behavioral effects, analysis of c-fos protein immunoreactivity (fos-ir) was used here to map areas activated by chronic CORT (200 mg pellets, 21-day release) and imipramine (15 mg/kg, IP) administration. We also evaluated the number of cells expressing the neurogenesis marker doublecortin (DCX) in the hippocampus and measured plasma CORT levels on the 21st day of treatment. Results showed that CORT increased fos-ir in the ventrolateral septum, medial amygdala and paraventricular hypothalamic nucleus and decreased fos-ir in the lateral periaqueductal gray. Imipramine, on the other hand, increased fos-ir in the medial amygdala and decreased fos-ir in the anterior hypothalamus. CORT also decreased the number of DCX-positive cells in the ventral and dorsal hippocampus, an effect antagonized by imipramine. CORT levels were significantly higher after treatment. These data suggest that the behavioral effects of CORT and imipramine are mediated through specific, at times overlapping, neuronal circuits, which might be of relevance to a better understanding of the physiopathology of generalized anxiety and panic disorder. SN - 1872-7549 UR - https://www.unboundmedicine.com/medline/citation/23098799/Effects_of_chronic_treatment_with_corticosterone_and_imipramine_on_fos_immunoreactivity_and_adult_hippocampal_neurogenesis_ DB - PRIME DP - Unbound Medicine ER -