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The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia.
Neuropharmacology. 2013 Feb; 65:206-12.N

Abstract

Transient receptor potential ankyrin 1 (TRPA1) is a nonselective cation channel important in setting nociceptive threshold. It is expressed in nociceptive C-fibers and in non-neuronal cells involved in pro-inflammatory mediators' release. We asked whether TRPA1 contributes to carrageenan-induced hyperalgesia in rats, and if so, whether this contribution is mediated by mechanisms involved in inflammation such as cytokine release and neutrophil migration and/or by a direct sensitization of the primary afferent nociceptors. Pharmacological blockade of local TRPA1 by its selective antagonist HC 030031 prevented and reversed carrageenan-induced hyperalgesia, which was detected either by a mechanical or chemical (low dose of capsaicin) stimulus. However, it did not affect either carrageenan-induced cytokines expression or neutrophil migration. The neuronal TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodoexynucleotide completely prevented carrageenan-induced hyperalgesia over 24 h and significantly reduced TRPA1 expression in the dorsal root ganglia cells (L5-6), which was not affected by carrageenan treatment. We conclude that TRPA1 plays an important role in the development and maintenance of carrageenan-induced inflammatory hyperalgesia by directly contributing to nociceptor excitability.

Authors+Show Affiliations

Department of Functional and Structural Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23098993

Citation

Bonet, Ivan J M., et al. "The Role of Transient Receptor Potential a 1 (TRPA1) in the Development and Maintenance of Carrageenan-induced Hyperalgesia." Neuropharmacology, vol. 65, 2013, pp. 206-12.
Bonet IJ, Fischer L, Parada CA, et al. The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia. Neuropharmacology. 2013;65:206-12.
Bonet, I. J., Fischer, L., Parada, C. A., & Tambeli, C. H. (2013). The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia. Neuropharmacology, 65, 206-12. https://doi.org/10.1016/j.neuropharm.2012.09.020
Bonet IJ, et al. The Role of Transient Receptor Potential a 1 (TRPA1) in the Development and Maintenance of Carrageenan-induced Hyperalgesia. Neuropharmacology. 2013;65:206-12. PubMed PMID: 23098993.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of transient receptor potential A 1 (TRPA1) in the development and maintenance of carrageenan-induced hyperalgesia. AU - Bonet,Ivan J M, AU - Fischer,Luana, AU - Parada,Carlos Amílcar, AU - Tambeli,Claudia H, Y1 - 2012/10/23/ PY - 2012/06/19/received PY - 2012/08/27/revised PY - 2012/09/17/accepted PY - 2012/10/27/entrez PY - 2012/10/27/pubmed PY - 2013/10/18/medline SP - 206 EP - 12 JF - Neuropharmacology JO - Neuropharmacology VL - 65 N2 - Transient receptor potential ankyrin 1 (TRPA1) is a nonselective cation channel important in setting nociceptive threshold. It is expressed in nociceptive C-fibers and in non-neuronal cells involved in pro-inflammatory mediators' release. We asked whether TRPA1 contributes to carrageenan-induced hyperalgesia in rats, and if so, whether this contribution is mediated by mechanisms involved in inflammation such as cytokine release and neutrophil migration and/or by a direct sensitization of the primary afferent nociceptors. Pharmacological blockade of local TRPA1 by its selective antagonist HC 030031 prevented and reversed carrageenan-induced hyperalgesia, which was detected either by a mechanical or chemical (low dose of capsaicin) stimulus. However, it did not affect either carrageenan-induced cytokines expression or neutrophil migration. The neuronal TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodoexynucleotide completely prevented carrageenan-induced hyperalgesia over 24 h and significantly reduced TRPA1 expression in the dorsal root ganglia cells (L5-6), which was not affected by carrageenan treatment. We conclude that TRPA1 plays an important role in the development and maintenance of carrageenan-induced inflammatory hyperalgesia by directly contributing to nociceptor excitability. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/23098993/The_role_of_transient_receptor_potential_A_1__TRPA1__in_the_development_and_maintenance_of_carrageenan_induced_hyperalgesia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(12)00511-4 DB - PRIME DP - Unbound Medicine ER -