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Poly(alizarin red)/graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine.
Anal Chim Acta. 2012 Nov 08; 752:94-100.AC

Abstract

In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ba X
Department of Chemistry, Shanghai University, PR China.
Luo L
No affiliation info available
Ding Y
No affiliation info available
Zhang Z
No affiliation info available
Chu Y
No affiliation info available
Wang B
No affiliation info available
Ouyang X
No affiliation info available

MeSH

AnthraquinonesCarbonElectrodesGlassGraphitePolymersPurinesPyrimidines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23101657

Citation

Ba, Xi, et al. "Poly(alizarin Red)/graphene Modified Glassy Carbon Electrode for Simultaneous Determination of Purine and Pyrimidine." Analytica Chimica Acta, vol. 752, 2012, pp. 94-100.
Ba X, Luo L, Ding Y, et al. Poly(alizarin red)/graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine. Anal Chim Acta. 2012;752:94-100.
Ba, X., Luo, L., Ding, Y., Zhang, Z., Chu, Y., Wang, B., & Ouyang, X. (2012). Poly(alizarin red)/graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine. Analytica Chimica Acta, 752, 94-100. https://doi.org/10.1016/j.aca.2012.09.031
Ba X, et al. Poly(alizarin Red)/graphene Modified Glassy Carbon Electrode for Simultaneous Determination of Purine and Pyrimidine. Anal Chim Acta. 2012 Nov 8;752:94-100. PubMed PMID: 23101657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poly(alizarin red)/graphene modified glassy carbon electrode for simultaneous determination of purine and pyrimidine. AU - Ba,Xi, AU - Luo,Liqiang, AU - Ding,Yaping, AU - Zhang,Zhen, AU - Chu,Yuliang, AU - Wang,Bijun, AU - Ouyang,Xiaoqian, Y1 - 2012/09/27/ PY - 2012/04/16/received PY - 2012/09/12/revised PY - 2012/09/16/accepted PY - 2012/10/30/entrez PY - 2012/10/30/pubmed PY - 2013/3/30/medline SP - 94 EP - 100 JF - Analytica chimica acta JO - Anal Chim Acta VL - 752 N2 - In this work, a poly(alizarin red)/Graphene composite film modified glassy carbon electrode (PAR/Graphene/GCE) was prepared for simultaneous determination of four DNA bases (guanine, adenine, thymine and cytosine) without any pretreatment. The morphology and interface property of PAR/Graphene films were examined by scanning electron microscopy and electrochemical impedance spectroscopy. The PAR/Graphene/GCE exhibited excellent electrocatalytic activity toward purine (guanine and adenine) and pyrimidine (thymine and cytosine) in 0.1 M phosphate buffer solution (pH 7.4). Under optimum conditions, differential pulse voltammetry was used to detect the oxidation of purine and pyrimidine. The results showed that PAR/Graphene/GCE exhibited well-separated peaks, low detection limit, high sensitivity and wide linear range for simultaneous detection of purine and pyrimidine. The proposed sensor also has good stability and reproducibility. Furthermore, the modified electrode was applied for the detection of DNA bases in a fish sperm DNA sample with satisfactory results. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/23101657/Poly_alizarin_red_/graphene_modified_glassy_carbon_electrode_for_simultaneous_determination_of_purine_and_pyrimidine_ DB - PRIME DP - Unbound Medicine ER -