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Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia.
Methods Mol Biol. 2013; 943:159-70.MM

Abstract

Pneumocystis jirovecii is a common cause of life-threatening pneumonia among immunocompromised patients. Since P. jirovecii cannot be cultured, specific identification of it depends on examining respiratory specimens. In the last decade, PCR has been developed which allows the detection of very low levels of P. jirovecii not detectable by routine histochemical staining. We have shown that the direct immunofluorescence assay can be replaced by a real-time PCR assay given its feasibility, sensitivity, and specificity, for the detection of P. jirovecii. A negative PCR, performed on a LightCycler System(®), enables a diagnosis of Pneumocystis jirovecii pneumonia (PjP) to be excluded, and the semiquantitative result with the application of some cutoff values can have a role in distinguishing between colonized or subclinically infected patients and PjP patients.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Fillaux J
Service de Parasitologie-Mycologie, Hôpital Rangueil, CHU Toulouse, Toulouse, France. fillaux@cict.fr
Berry A
No affiliation info available

MeSH

DNA, BacterialHumansPneumocystis cariniiPneumonia, PneumocystisReal-Time Polymerase Chain ReactionSensitivity and SpecificitySpecimen Handlingbeta-Globins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23104289

Citation

Fillaux, Judith, and Antoine Berry. "Real-time PCR Assay for the Diagnosis of Pneumocystis Jirovecii Pneumonia." Methods in Molecular Biology (Clifton, N.J.), vol. 943, 2013, pp. 159-70.
Fillaux J, Berry A. Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. Methods Mol Biol. 2013;943:159-70.
Fillaux, J., & Berry, A. (2013). Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. Methods in Molecular Biology (Clifton, N.J.), 943, 159-70. https://doi.org/10.1007/978-1-60327-353-4_11
Fillaux J, Berry A. Real-time PCR Assay for the Diagnosis of Pneumocystis Jirovecii Pneumonia. Methods Mol Biol. 2013;943:159-70. PubMed PMID: 23104289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Real-time PCR assay for the diagnosis of Pneumocystis jirovecii pneumonia. AU - Fillaux,Judith, AU - Berry,Antoine, PY - 2012/10/30/entrez PY - 2012/10/30/pubmed PY - 2013/3/30/medline SP - 159 EP - 70 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 943 N2 - Pneumocystis jirovecii is a common cause of life-threatening pneumonia among immunocompromised patients. Since P. jirovecii cannot be cultured, specific identification of it depends on examining respiratory specimens. In the last decade, PCR has been developed which allows the detection of very low levels of P. jirovecii not detectable by routine histochemical staining. We have shown that the direct immunofluorescence assay can be replaced by a real-time PCR assay given its feasibility, sensitivity, and specificity, for the detection of P. jirovecii. A negative PCR, performed on a LightCycler System(®), enables a diagnosis of Pneumocystis jirovecii pneumonia (PjP) to be excluded, and the semiquantitative result with the application of some cutoff values can have a role in distinguishing between colonized or subclinically infected patients and PjP patients. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/23104289/Real_time_PCR_assay_for_the_diagnosis_of_Pneumocystis_jirovecii_pneumonia_ L2 - https://dx.doi.org/10.1007/978-1-60327-353-4_11 DB - PRIME DP - Unbound Medicine ER -