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The effect of adenylate cyclase stimulation on endocochlear potential in the guinea pig.
Eur Arch Otorhinolaryngol. 1990; 247(1):16-9.EA

Abstract

Forskolin, a diterpene extracted from Coleus forskohlii, is potentially an important tool for studying the modulation of ionic currents by cAMP because it stimulates adenylate cyclase in a variety of cells. We studied the effect of forskolin on cochlear potentials and found that its perfusion of the scala vestibuli (SV) to a concentration more than 10(-5) M and the scala tympani (ST) to more than 10(-4) M produced a reversible elevation of the endocochlear potential (EP) in a dose-dependent manner. The cochlear microphonics recorded simultaneously with the EP was not depressed during the EP elevation. A large negative EP was induced by anoxia following the SV perfusion with forskolin (2 X 10(-4) M). The results suggest that the EP elevation produced by forskolin does not result from the decrease in the negative component of EP but from the increase in the positive component of EP.

Authors+Show Affiliations

Department of Otolaryngology, Osaka University Medical School, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2310543

Citation

Doi, K, et al. "The Effect of Adenylate Cyclase Stimulation On Endocochlear Potential in the Guinea Pig." European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, vol. 247, no. 1, 1990, pp. 16-9.
Doi K, Mori N, Matsunaga T. The effect of adenylate cyclase stimulation on endocochlear potential in the guinea pig. Eur Arch Otorhinolaryngol. 1990;247(1):16-9.
Doi, K., Mori, N., & Matsunaga, T. (1990). The effect of adenylate cyclase stimulation on endocochlear potential in the guinea pig. European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated With the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 247(1), 16-9.
Doi K, Mori N, Matsunaga T. The Effect of Adenylate Cyclase Stimulation On Endocochlear Potential in the Guinea Pig. Eur Arch Otorhinolaryngol. 1990;247(1):16-9. PubMed PMID: 2310543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of adenylate cyclase stimulation on endocochlear potential in the guinea pig. AU - Doi,K, AU - Mori,N, AU - Matsunaga,T, PY - 1990/1/1/pubmed PY - 1990/1/1/medline PY - 1990/1/1/entrez SP - 16 EP - 9 JF - European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery JO - Eur Arch Otorhinolaryngol VL - 247 IS - 1 N2 - Forskolin, a diterpene extracted from Coleus forskohlii, is potentially an important tool for studying the modulation of ionic currents by cAMP because it stimulates adenylate cyclase in a variety of cells. We studied the effect of forskolin on cochlear potentials and found that its perfusion of the scala vestibuli (SV) to a concentration more than 10(-5) M and the scala tympani (ST) to more than 10(-4) M produced a reversible elevation of the endocochlear potential (EP) in a dose-dependent manner. The cochlear microphonics recorded simultaneously with the EP was not depressed during the EP elevation. A large negative EP was induced by anoxia following the SV perfusion with forskolin (2 X 10(-4) M). The results suggest that the EP elevation produced by forskolin does not result from the decrease in the negative component of EP but from the increase in the positive component of EP. SN - 0937-4477 UR - https://www.unboundmedicine.com/medline/citation/2310543/The_effect_of_adenylate_cyclase_stimulation_on_endocochlear_potential_in_the_guinea_pig_ DB - PRIME DP - Unbound Medicine ER -