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Effects of homocysteine on ERK signaling and cell proliferation in fetal neural stem cells in vitro.
Cell Biochem Biophys. 2013 May; 66(1):131-7.CB

Abstract

The aim of the present study was to determine if the excitatory amino acid homocysteine (Hcy) alters ERK signaling and cell proliferation in fetal neural stem cells (NSCs) in vitro. NSCs were isolated from fetal rats and grown in serum-free suspension medium. The cells were identified as NSCs by their expression of immunoreactive Sox2. NSCs were assigned to one of four treatment groups: vehicle control, low-dose Hcy group (Hcy-L, medium contained 30 μmol/L Hcy), middle-dose Hcy group (Hcy-M, 100 μmol/L Hcy) and high-dose Hcy group (Hcy-H, 300 μmol/L Hcy). Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Protein expression levels of ERK1/2 and phosphorylated ERK1/2 were detected by Western blot. The effects of Hcy on NSC death, including apoptosis, were assessed by using flow cytometry and trypan blue exclusion. The results showed that NSCs grew as neurospheres in the serum-free medium. Hcy decreased ERK1/2 protein phosphorylation and NSC proliferation, but it did not induce cell death or apoptosis within the concentration from 30 to 300 μmol/L. The above results are consistent with the hypothesis that Hcy decreases fetal NSC proliferation by inhibiting ERK signaling.

Authors+Show Affiliations

Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin, 300070, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23109178

Citation

Yan, Hai, et al. "Effects of Homocysteine On ERK Signaling and Cell Proliferation in Fetal Neural Stem Cells in Vitro." Cell Biochemistry and Biophysics, vol. 66, no. 1, 2013, pp. 131-7.
Yan H, Zhang X, Luo S, et al. Effects of homocysteine on ERK signaling and cell proliferation in fetal neural stem cells in vitro. Cell Biochem Biophys. 2013;66(1):131-7.
Yan, H., Zhang, X., Luo, S., Liu, H., Wang, X., Gao, Y., Wilson, J. X., & Huang, G. (2013). Effects of homocysteine on ERK signaling and cell proliferation in fetal neural stem cells in vitro. Cell Biochemistry and Biophysics, 66(1), 131-7. https://doi.org/10.1007/s12013-012-9461-z
Yan H, et al. Effects of Homocysteine On ERK Signaling and Cell Proliferation in Fetal Neural Stem Cells in Vitro. Cell Biochem Biophys. 2013;66(1):131-7. PubMed PMID: 23109178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of homocysteine on ERK signaling and cell proliferation in fetal neural stem cells in vitro. AU - Yan,Hai, AU - Zhang,Xumei, AU - Luo,Suhui, AU - Liu,Huan, AU - Wang,Xuan, AU - Gao,Yuxia, AU - Wilson,John X, AU - Huang,Guowei, PY - 2012/10/31/entrez PY - 2012/10/31/pubmed PY - 2013/12/20/medline SP - 131 EP - 7 JF - Cell biochemistry and biophysics JO - Cell Biochem. Biophys. VL - 66 IS - 1 N2 - The aim of the present study was to determine if the excitatory amino acid homocysteine (Hcy) alters ERK signaling and cell proliferation in fetal neural stem cells (NSCs) in vitro. NSCs were isolated from fetal rats and grown in serum-free suspension medium. The cells were identified as NSCs by their expression of immunoreactive Sox2. NSCs were assigned to one of four treatment groups: vehicle control, low-dose Hcy group (Hcy-L, medium contained 30 μmol/L Hcy), middle-dose Hcy group (Hcy-M, 100 μmol/L Hcy) and high-dose Hcy group (Hcy-H, 300 μmol/L Hcy). Cell proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Protein expression levels of ERK1/2 and phosphorylated ERK1/2 were detected by Western blot. The effects of Hcy on NSC death, including apoptosis, were assessed by using flow cytometry and trypan blue exclusion. The results showed that NSCs grew as neurospheres in the serum-free medium. Hcy decreased ERK1/2 protein phosphorylation and NSC proliferation, but it did not induce cell death or apoptosis within the concentration from 30 to 300 μmol/L. The above results are consistent with the hypothesis that Hcy decreases fetal NSC proliferation by inhibiting ERK signaling. SN - 1559-0283 UR - https://www.unboundmedicine.com/medline/citation/23109178/Effects_of_homocysteine_on_ERK_signaling_and_cell_proliferation_in_fetal_neural_stem_cells_in_vitro_ L2 - https://dx.doi.org/10.1007/s12013-012-9461-z DB - PRIME DP - Unbound Medicine ER -