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Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone.
Indian J Pharm Sci. 2011 Nov; 73(6):696-9.IJ

Abstract

A new simple, selective, rapid, precise and accurate reverse phase HPLC method has been developed for simultaneous estimation of granisetron and dexamethasone. The method was developed using CPS Hypersil CN column (250×4.6 mm I.D.) with a mobile phase consisting of acetonitrile:buffer (100 mM Triethylamine adjusted to pH 3.0 with o-phosphoric acid) in ratio of 25:75 at a flow rate of 2 ml/min. Detection was carried out at 242 nm. The developed method was evaluated for various system suitability parameters and validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. It was also evaluated for bench top stability and freeze/thaw stability. The proposed method can be used for the estimation of these drugs in their combined dosage forms.

Authors+Show Affiliations

Department of Pharmaceutics, Government College of Pharmacy, Osmanpura, Vedant Road, Aurangabad-431 005, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23112409

Citation

Heda, A A., et al. "Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone." Indian Journal of Pharmaceutical Sciences, vol. 73, no. 6, 2011, pp. 696-9.
Heda AA, Kathiriya JM, Gadade DD, et al. Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone. Indian J Pharm Sci. 2011;73(6):696-9.
Heda, A. A., Kathiriya, J. M., Gadade, D. D., & Puranik, P. K. (2011). Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone. Indian Journal of Pharmaceutical Sciences, 73(6), 696-9. https://doi.org/10.4103/0250-474X.100255
Heda AA, et al. Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone. Indian J Pharm Sci. 2011;73(6):696-9. PubMed PMID: 23112409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and Validation of RP-HPLC Method for Simultaneous Determination of Granisetron and Dexamethasone. AU - Heda,A A, AU - Kathiriya,J M, AU - Gadade,D D, AU - Puranik,P K, PY - 2010/07/19/received PY - 2011/11/28/revised PY - 2011/12/05/accepted PY - 2012/11/1/entrez PY - 2012/11/1/pubmed PY - 2012/11/1/medline KW - RP-HPLC KW - dexamethasone KW - granisetron KW - validation SP - 696 EP - 9 JF - Indian journal of pharmaceutical sciences JO - Indian J Pharm Sci VL - 73 IS - 6 N2 - A new simple, selective, rapid, precise and accurate reverse phase HPLC method has been developed for simultaneous estimation of granisetron and dexamethasone. The method was developed using CPS Hypersil CN column (250×4.6 mm I.D.) with a mobile phase consisting of acetonitrile:buffer (100 mM Triethylamine adjusted to pH 3.0 with o-phosphoric acid) in ratio of 25:75 at a flow rate of 2 ml/min. Detection was carried out at 242 nm. The developed method was evaluated for various system suitability parameters and validated for linearity, accuracy, precision, LOD, LOQ as per ICH guidelines. It was also evaluated for bench top stability and freeze/thaw stability. The proposed method can be used for the estimation of these drugs in their combined dosage forms. SN - 1998-3743 UR - https://www.unboundmedicine.com/medline/citation/23112409/Development_and_Validation_of_RP_HPLC_Method_for_Simultaneous_Determination_of_Granisetron_and_Dexamethasone_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23112409/ DB - PRIME DP - Unbound Medicine ER -
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