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Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey.
Antimicrob Agents Chemother. 2013 Jan; 57(1):269-76.AA

Abstract

Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla(KPC), commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla(KPC-2) and bla(KPC-3), identified in plasmids with diverse genetic backgrounds. In this study, we examined bla(KPC-4)- and bla(KPC-5)-bearing plasmids recovered from two K. pneumoniae strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors bla(KPC-4), bla(TEM-1), qnrB2, aac(3)-Ib, aph(3')-I, qacF, qacEΔ1, sul1, and dfrA14, which confer resistance to β-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn4401 sequence revealed a large IS110- and Tn6901-carrying element (8.3 kb) inserted into the istA gene, encoding glyoxalase/bleomycin resistance, alcohol dehydrogenase, and S-formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors bla(KPC-5), dfrA5, qacEΔ1, and sul1. pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The bla(KPC-5) gene is carried on a Tn4401 element and differs from the genetic environment of bla(KPC-5) described in Pseudomonas aeruginosa strain P28 from Puerto Rico. This study underscores the genetic diversity of multidrug-resistant plasmids involved in the spread of bla(KPC) genes and highlights the mobility and plasticity of Tn4401. Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups.

Authors+Show Affiliations

Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23114770

Citation

Chen, Liang, et al. "Complete Nucleotide Sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX Plasmids From Klebsiella Pneumoniae Strains Isolated in New Jersey." Antimicrobial Agents and Chemotherapy, vol. 57, no. 1, 2013, pp. 269-76.
Chen L, Chavda KD, Fraimow HS, et al. Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. Antimicrob Agents Chemother. 2013;57(1):269-76.
Chen, L., Chavda, K. D., Fraimow, H. S., Mediavilla, J. R., Melano, R. G., Jacobs, M. R., Bonomo, R. A., & Kreiswirth, B. N. (2013). Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. Antimicrobial Agents and Chemotherapy, 57(1), 269-76. https://doi.org/10.1128/AAC.01648-12
Chen L, et al. Complete Nucleotide Sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX Plasmids From Klebsiella Pneumoniae Strains Isolated in New Jersey. Antimicrob Agents Chemother. 2013;57(1):269-76. PubMed PMID: 23114770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. AU - Chen,Liang, AU - Chavda,Kalyan D, AU - Fraimow,Henry S, AU - Mediavilla,José R, AU - Melano,Roberto G, AU - Jacobs,Michael R, AU - Bonomo,Robert A, AU - Kreiswirth,Barry N, Y1 - 2012/10/31/ PY - 2012/11/2/entrez PY - 2012/11/2/pubmed PY - 2013/6/12/medline SP - 269 EP - 76 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 57 IS - 1 N2 - Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have emerged as major nosocomial pathogens. bla(KPC), commonly located on Tn4401, is found in Gram-negative bacterial strains, with the two most common variants, bla(KPC-2) and bla(KPC-3), identified in plasmids with diverse genetic backgrounds. In this study, we examined bla(KPC-4)- and bla(KPC-5)-bearing plasmids recovered from two K. pneumoniae strains, which were isolated from a single New Jersey hospital in 2005 and 2006, respectively. IncN plasmid pBK31551 is 84 kb in length and harbors bla(KPC-4), bla(TEM-1), qnrB2, aac(3)-Ib, aph(3')-I, qacF, qacEΔ1, sul1, and dfrA14, which confer resistance to β-lactams, quinolones, aminoglycosides, quaternary ammonium compounds, and co-trimoxazole. The conserved regions within pBK31551 are similar to those of other IncN plasmids. Surprisingly, analysis of the Tn4401 sequence revealed a large IS110- and Tn6901-carrying element (8.3 kb) inserted into the istA gene, encoding glyoxalase/bleomycin resistance, alcohol dehydrogenase, and S-formylglutathione hydrolase. Plasmid pBK31567 is 47 kb in length and harbors bla(KPC-5), dfrA5, qacEΔ1, and sul1. pBK31567 belongs to a novel IncX subgroup (IncX5) and possesses a highly syntenic plasmid backbone like other IncX plasmids; however, sequence similarity at the nucleotide level is divergent. The bla(KPC-5) gene is carried on a Tn4401 element and differs from the genetic environment of bla(KPC-5) described in Pseudomonas aeruginosa strain P28 from Puerto Rico. This study underscores the genetic diversity of multidrug-resistant plasmids involved in the spread of bla(KPC) genes and highlights the mobility and plasticity of Tn4401. Comparative genomic analysis provides new insights into the evolution and dissemination of KPC plasmids belonging to different incompatibility groups. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/23114770/Complete_nucleotide_sequences_of_blaKPC_4__and_blaKPC_5_harboring_IncN_and_IncX_plasmids_from_Klebsiella_pneumoniae_strains_isolated_in_New_Jersey_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=23114770 DB - PRIME DP - Unbound Medicine ER -