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Intracellular trehalase activity is required for development, germination and heat-stress resistance of Aspergillus niger conidia.
Res Microbiol. 2013 Feb-Mar; 164(2):91-9.RM

Abstract

The disaccharide trehalose is known as a stress protectant in several kinds of organisms, including fungi, where it is a major carbohydrate in resting structures, e.g. asexual conidia. The gene encoding the enzyme responsible for degradation of intracellular trehalose, treB, was deleted and the phenotype was analyzed in terms of morphology, trehalose content during conidial outgrowth and stress tolerance. The mutant conidiophores produced fewer and less viable spores, and during early stages of germination the internal levels of trehalose were higher compared to the wild type. When subjecting the mutant to various stresses (weak acid and salt), no increased sensitivity could be observed, but in line with previous observations, e.g. in Aspergillus nidulans, Aspergillus niger ΔtreB spores in a very early stage of germination were less sensitive to heat stress. In contrast, when subjecting resting spores to 55 °C, an intact treB gene was essential for survival. This finding suggests that trehalose mobilization is required to facilitate cell recovery after heat-induced damage.

Authors+Show Affiliations

Department of Microbiology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7025, SE-75007 Uppsala, Sweden. Asa.svanstrom@slu.seNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23116628

Citation

Svanström, Asa, and Petter Melin. "Intracellular Trehalase Activity Is Required for Development, Germination and Heat-stress Resistance of Aspergillus Niger Conidia." Research in Microbiology, vol. 164, no. 2, 2013, pp. 91-9.
Svanström A, Melin P. Intracellular trehalase activity is required for development, germination and heat-stress resistance of Aspergillus niger conidia. Res Microbiol. 2013;164(2):91-9.
Svanström, A., & Melin, P. (2013). Intracellular trehalase activity is required for development, germination and heat-stress resistance of Aspergillus niger conidia. Research in Microbiology, 164(2), 91-9. https://doi.org/10.1016/j.resmic.2012.10.018
Svanström A, Melin P. Intracellular Trehalase Activity Is Required for Development, Germination and Heat-stress Resistance of Aspergillus Niger Conidia. Res Microbiol. 2013 Feb-Mar;164(2):91-9. PubMed PMID: 23116628.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intracellular trehalase activity is required for development, germination and heat-stress resistance of Aspergillus niger conidia. AU - Svanström,Asa, AU - Melin,Petter, Y1 - 2012/10/29/ PY - 2012/05/29/received PY - 2012/10/15/accepted PY - 2012/11/3/entrez PY - 2012/11/3/pubmed PY - 2013/8/13/medline SP - 91 EP - 9 JF - Research in microbiology JO - Res. Microbiol. VL - 164 IS - 2 N2 - The disaccharide trehalose is known as a stress protectant in several kinds of organisms, including fungi, where it is a major carbohydrate in resting structures, e.g. asexual conidia. The gene encoding the enzyme responsible for degradation of intracellular trehalose, treB, was deleted and the phenotype was analyzed in terms of morphology, trehalose content during conidial outgrowth and stress tolerance. The mutant conidiophores produced fewer and less viable spores, and during early stages of germination the internal levels of trehalose were higher compared to the wild type. When subjecting the mutant to various stresses (weak acid and salt), no increased sensitivity could be observed, but in line with previous observations, e.g. in Aspergillus nidulans, Aspergillus niger ΔtreB spores in a very early stage of germination were less sensitive to heat stress. In contrast, when subjecting resting spores to 55 °C, an intact treB gene was essential for survival. This finding suggests that trehalose mobilization is required to facilitate cell recovery after heat-induced damage. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/23116628/Intracellular_trehalase_activity_is_required_for_development_germination_and_heat_stress_resistance_of_Aspergillus_niger_conidia_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(12)00163-5 DB - PRIME DP - Unbound Medicine ER -