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Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis.
J Clin Endocrinol Metab. 2013 Jan; 98(1):281-9.JC

Abstract

CONTEXT

There is currently no reliable noninvasive biomarker for the clinical diagnosis of endometriosis. Previous analyses have reported that circulating microRNAs (miRNAs) can serve as biomarkers for a number of diseases.

OBJECTIVE

The study aims to detect the serum miRNAs that are differentially expressed between endometriosis patients and negative controls to evaluate the potential of these miRNAs as diagnostic markers for endometriosis.

DESIGN

A total of 765 serum miRNAs were profiled using a TaqMan microRNA array in a pool of 10 endometriosis patients and a pool of 10 negative controls, and a set of selected miRNAs were further analyzed in a validation cohort consisting of sera from 60 patients and 25 controls including 10 samples used in array profiling.

RESULTS

The relative expression levels of miR-199a and miR-122 were found to be up-regulated in endometriosis patient samples compared with control samples, whereas miR-145*, miR-141*, miR-542-3p, and miR-9* were down-regulated in endometriosis patients. Importantly, the relative expression of miR-199a (P < 0.05) and miR-122 can be used to discriminate between severe and mild endometriosis. We also found that miR-199a is well correlated with pelvic adhesion and lesion distribution (P < 0.05) and associated with hormone-mediated signaling pathways. Furthermore, we investigated the diagnostic value of these molecules and confirmed the optimal combination of miR-199a, miR-122, miR-145*, and miR-542-3p with area under the curve of 0.994 (95% confidence interval = 0.984-1.000, P < 0.001) and a cutoff point (0.4950) of 93.22% sensitivity and 96.00% specificity.

CONCLUSIONS

Our study demonstrated that the circulating miRNAs miR-199a, miR-122, miR-145*, and miR-542-3p could potentially serve as noninvasive biomarkers for endometriosis. miR-199a may also play an important role in the progression of the disease. This is the first report that circulating miRNAs serve as biomarkers of endometriosis.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Sun Yat-sen Memorial Hospital, Guangzhou 510120, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23118427

Citation

Wang, Wen-Tao, et al. "Circulating microRNAs Identified in a Genome-wide Serum microRNA Expression Analysis as Noninvasive Biomarkers for Endometriosis." The Journal of Clinical Endocrinology and Metabolism, vol. 98, no. 1, 2013, pp. 281-9.
Wang WT, Zhao YN, Han BW, et al. Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis. J Clin Endocrinol Metab. 2013;98(1):281-9.
Wang, W. T., Zhao, Y. N., Han, B. W., Hong, S. J., & Chen, Y. Q. (2013). Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis. The Journal of Clinical Endocrinology and Metabolism, 98(1), 281-9. https://doi.org/10.1210/jc.2012-2415
Wang WT, et al. Circulating microRNAs Identified in a Genome-wide Serum microRNA Expression Analysis as Noninvasive Biomarkers for Endometriosis. J Clin Endocrinol Metab. 2013;98(1):281-9. PubMed PMID: 23118427.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Circulating microRNAs identified in a genome-wide serum microRNA expression analysis as noninvasive biomarkers for endometriosis. AU - Wang,Wen-Tao, AU - Zhao,Ya-Nan, AU - Han,Bo-Wei, AU - Hong,Shun-Jia, AU - Chen,Yue-Qin, Y1 - 2012/11/01/ PY - 2012/11/3/entrez PY - 2012/11/3/pubmed PY - 2013/3/8/medline SP - 281 EP - 9 JF - The Journal of clinical endocrinology and metabolism JO - J. Clin. Endocrinol. Metab. VL - 98 IS - 1 N2 - CONTEXT: There is currently no reliable noninvasive biomarker for the clinical diagnosis of endometriosis. Previous analyses have reported that circulating microRNAs (miRNAs) can serve as biomarkers for a number of diseases. OBJECTIVE: The study aims to detect the serum miRNAs that are differentially expressed between endometriosis patients and negative controls to evaluate the potential of these miRNAs as diagnostic markers for endometriosis. DESIGN: A total of 765 serum miRNAs were profiled using a TaqMan microRNA array in a pool of 10 endometriosis patients and a pool of 10 negative controls, and a set of selected miRNAs were further analyzed in a validation cohort consisting of sera from 60 patients and 25 controls including 10 samples used in array profiling. RESULTS: The relative expression levels of miR-199a and miR-122 were found to be up-regulated in endometriosis patient samples compared with control samples, whereas miR-145*, miR-141*, miR-542-3p, and miR-9* were down-regulated in endometriosis patients. Importantly, the relative expression of miR-199a (P < 0.05) and miR-122 can be used to discriminate between severe and mild endometriosis. We also found that miR-199a is well correlated with pelvic adhesion and lesion distribution (P < 0.05) and associated with hormone-mediated signaling pathways. Furthermore, we investigated the diagnostic value of these molecules and confirmed the optimal combination of miR-199a, miR-122, miR-145*, and miR-542-3p with area under the curve of 0.994 (95% confidence interval = 0.984-1.000, P < 0.001) and a cutoff point (0.4950) of 93.22% sensitivity and 96.00% specificity. CONCLUSIONS: Our study demonstrated that the circulating miRNAs miR-199a, miR-122, miR-145*, and miR-542-3p could potentially serve as noninvasive biomarkers for endometriosis. miR-199a may also play an important role in the progression of the disease. This is the first report that circulating miRNAs serve as biomarkers of endometriosis. SN - 1945-7197 UR - https://www.unboundmedicine.com/medline/citation/23118427/Circulating_microRNAs_identified_in_a_genome_wide_serum_microRNA_expression_analysis_as_noninvasive_biomarkers_for_endometriosis_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2012-2415 DB - PRIME DP - Unbound Medicine ER -