TY - JOUR T1 - An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits. AU - De Paepe,D, AU - Servaes,K, AU - Noten,B, AU - Diels,L, AU - De Loose,M, AU - Van Droogenbroeck,B, AU - Voorspoels,S, Y1 - 2012/09/07/ PY - 2011/08/28/received PY - 2012/05/24/revised PY - 2012/08/29/accepted PY - 2012/11/6/entrez PY - 2012/11/6/pubmed PY - 2013/5/23/medline SP - 368 EP - 75 JF - Food chemistry JO - Food Chem VL - 136 IS - 2 N2 - Thirty-nine phenolic compounds were analysed using ultra high performance liquid chromatography (UHPLC) coupled with diode array and accurate mass spectrometry detection using electrospray ionisation (DAD/ESI-am-MS). Instrumental parameters such as scan speed, resolution, and mass accuracy were optimised to establish accurate mass measurements. The method was fully validated in terms of model deviation (r(2)>0.9990), range (typically 10-3500 ngg(-1)), intra/inter-day precision (<6% and <8%, respectively) and accuracy (typically 100 ± 10%). The mass accuracy of each selected phenolic compound was below 1.5 ppm. The results confirmed that the UHPLC-DAD/ESI-am-MS method developed here was convenient and reliable for the determination of phenolic compounds in apple extracts. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23122072/An_improved_mass_spectrometric_method_for_identification_and_quantification_of_phenolic_compounds_in_apple_fruits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)01356-8 DB - PRIME DP - Unbound Medicine ER -