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An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits.
Food Chem. 2013 Jan 15; 136(2):368-75.FC

Abstract

Thirty-nine phenolic compounds were analysed using ultra high performance liquid chromatography (UHPLC) coupled with diode array and accurate mass spectrometry detection using electrospray ionisation (DAD/ESI-am-MS). Instrumental parameters such as scan speed, resolution, and mass accuracy were optimised to establish accurate mass measurements. The method was fully validated in terms of model deviation (r(2)>0.9990), range (typically 10-3500 ngg(-1)), intra/inter-day precision (<6% and <8%, respectively) and accuracy (typically 100 ± 10%). The mass accuracy of each selected phenolic compound was below 1.5 ppm. The results confirmed that the UHPLC-DAD/ESI-am-MS method developed here was convenient and reliable for the determination of phenolic compounds in apple extracts.

Authors+Show Affiliations

Flemish Institute for Technological Research (VITO), Environmental Analysis and Technology, Boeretang 200, 2400 Mol, Belgium; Institute domien.depaepe@ilvo.vlaanderen.beNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

23122072

Citation

De Paepe, D, et al. "An Improved Mass Spectrometric Method for Identification and Quantification of Phenolic Compounds in Apple Fruits." Food Chemistry, vol. 136, no. 2, 2013, pp. 368-75.
De Paepe D, Servaes K, Noten B, et al. An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits. Food Chem. 2013;136(2):368-75.
De Paepe, D., Servaes, K., Noten, B., Diels, L., De Loose, M., Van Droogenbroeck, B., & Voorspoels, S. (2013). An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits. Food Chemistry, 136(2), 368-75. https://doi.org/10.1016/j.foodchem.2012.08.062
De Paepe D, et al. An Improved Mass Spectrometric Method for Identification and Quantification of Phenolic Compounds in Apple Fruits. Food Chem. 2013 Jan 15;136(2):368-75. PubMed PMID: 23122072.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits. AU - De Paepe,D, AU - Servaes,K, AU - Noten,B, AU - Diels,L, AU - De Loose,M, AU - Van Droogenbroeck,B, AU - Voorspoels,S, Y1 - 2012/09/07/ PY - 2011/08/28/received PY - 2012/05/24/revised PY - 2012/08/29/accepted PY - 2012/11/6/entrez PY - 2012/11/6/pubmed PY - 2013/5/23/medline SP - 368 EP - 75 JF - Food chemistry JO - Food Chem VL - 136 IS - 2 N2 - Thirty-nine phenolic compounds were analysed using ultra high performance liquid chromatography (UHPLC) coupled with diode array and accurate mass spectrometry detection using electrospray ionisation (DAD/ESI-am-MS). Instrumental parameters such as scan speed, resolution, and mass accuracy were optimised to establish accurate mass measurements. The method was fully validated in terms of model deviation (r(2)>0.9990), range (typically 10-3500 ngg(-1)), intra/inter-day precision (<6% and <8%, respectively) and accuracy (typically 100 ± 10%). The mass accuracy of each selected phenolic compound was below 1.5 ppm. The results confirmed that the UHPLC-DAD/ESI-am-MS method developed here was convenient and reliable for the determination of phenolic compounds in apple extracts. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23122072/An_improved_mass_spectrometric_method_for_identification_and_quantification_of_phenolic_compounds_in_apple_fruits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(12)01356-8 DB - PRIME DP - Unbound Medicine ER -