Tags

Type your tag names separated by a space and hit enter

The role of calcitonin gene-related peptide on the increase in transient receptor potential vanilloid-1 levels in trigeminal ganglion and trigeminal nucleus caudalis activation of rat.
J Chem Neuroanat. 2013 Jan; 47:50-6.JC

Abstract

Calcitonin gene-related peptide (CGRP) and transient receptor potential vanilloid-1 (TRPV1) play an important role in the development of pain and migraine pathogenesis. Increase in plasma CGRP levels is associated with delayed migraine-like attacks in migraine patients. Although several lines of evidence have indicated a key role of CGRP in migraine pain, its mechanisms remain unclear. In this study, we aimed to investigate the functional role of CGRP on trigeminal nociceptive pathway by determining the alteration in TRPV1 levels in trigeminal ganglion (TG) and the activation of trigeminal nucleus caudalis (TNC) of rat. Post intravenous injection of CGRP (600ng/kg) at 60min significantly increased the levels of TRPV1, CGRP, phosphorylated protein kinase C and phosphorylated cyclic AMP responsive element-binding protein in TG of rats. The number of small and medium TRPV1 and CGRP positive immunostaining neurons accompanying with co-localization of TRPV1 with CGRP neurons were significantly increased in TG of CGRP-injected rats. The sustained increase in c-Fos expression in TNC neurons was also observed in CGRP-injected rats. These results indicate that CGRP may participate in trigeminal nociceptive system sensitization by induced increase in TRPV1 and CGRP levels in TG neurons and activation of the central neurons in TNC.

Authors+Show Affiliations

Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom 73170, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23123284

Citation

Chatchaisak, Duangthip, et al. "The Role of Calcitonin Gene-related Peptide On the Increase in Transient Receptor Potential Vanilloid-1 Levels in Trigeminal Ganglion and Trigeminal Nucleus Caudalis Activation of Rat." Journal of Chemical Neuroanatomy, vol. 47, 2013, pp. 50-6.
Chatchaisak D, Srikiatkhachorn A, Maneesri-le Grand S, et al. The role of calcitonin gene-related peptide on the increase in transient receptor potential vanilloid-1 levels in trigeminal ganglion and trigeminal nucleus caudalis activation of rat. J Chem Neuroanat. 2013;47:50-6.
Chatchaisak, D., Srikiatkhachorn, A., Maneesri-le Grand, S., Govitrapong, P., & Chetsawang, B. (2013). The role of calcitonin gene-related peptide on the increase in transient receptor potential vanilloid-1 levels in trigeminal ganglion and trigeminal nucleus caudalis activation of rat. Journal of Chemical Neuroanatomy, 47, 50-6. https://doi.org/10.1016/j.jchemneu.2012.09.005
Chatchaisak D, et al. The Role of Calcitonin Gene-related Peptide On the Increase in Transient Receptor Potential Vanilloid-1 Levels in Trigeminal Ganglion and Trigeminal Nucleus Caudalis Activation of Rat. J Chem Neuroanat. 2013;47:50-6. PubMed PMID: 23123284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of calcitonin gene-related peptide on the increase in transient receptor potential vanilloid-1 levels in trigeminal ganglion and trigeminal nucleus caudalis activation of rat. AU - Chatchaisak,Duangthip, AU - Srikiatkhachorn,Anan, AU - Maneesri-le Grand,Supang, AU - Govitrapong,Piyarat, AU - Chetsawang,Banthit, Y1 - 2012/11/02/ PY - 2012/07/30/received PY - 2012/09/27/revised PY - 2012/09/28/accepted PY - 2012/11/6/entrez PY - 2012/11/6/pubmed PY - 2013/8/10/medline SP - 50 EP - 6 JF - Journal of chemical neuroanatomy JO - J. Chem. Neuroanat. VL - 47 N2 - Calcitonin gene-related peptide (CGRP) and transient receptor potential vanilloid-1 (TRPV1) play an important role in the development of pain and migraine pathogenesis. Increase in plasma CGRP levels is associated with delayed migraine-like attacks in migraine patients. Although several lines of evidence have indicated a key role of CGRP in migraine pain, its mechanisms remain unclear. In this study, we aimed to investigate the functional role of CGRP on trigeminal nociceptive pathway by determining the alteration in TRPV1 levels in trigeminal ganglion (TG) and the activation of trigeminal nucleus caudalis (TNC) of rat. Post intravenous injection of CGRP (600ng/kg) at 60min significantly increased the levels of TRPV1, CGRP, phosphorylated protein kinase C and phosphorylated cyclic AMP responsive element-binding protein in TG of rats. The number of small and medium TRPV1 and CGRP positive immunostaining neurons accompanying with co-localization of TRPV1 with CGRP neurons were significantly increased in TG of CGRP-injected rats. The sustained increase in c-Fos expression in TNC neurons was also observed in CGRP-injected rats. These results indicate that CGRP may participate in trigeminal nociceptive system sensitization by induced increase in TRPV1 and CGRP levels in TG neurons and activation of the central neurons in TNC. SN - 1873-6300 UR - https://www.unboundmedicine.com/medline/citation/23123284/The_role_of_calcitonin_gene_related_peptide_on_the_increase_in_transient_receptor_potential_vanilloid_1_levels_in_trigeminal_ganglion_and_trigeminal_nucleus_caudalis_activation_of_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-0618(12)00065-8 DB - PRIME DP - Unbound Medicine ER -