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Crucial role of the N-glycans on the viral E-envelope glycoprotein in DC-SIGN-mediated dengue virus infection.
Antiviral Res. 2012 Dec; 96(3):280-7.AR

Abstract

We generated in the mosquito cell line C6/36 a dengue virus (DENV) resistant to Hippeastrum hybrid agglutinin (HHA), a carbohydrate-binding agent (CBA). The genotype and phenotype were characterized of the HHA resistant (HHA(res)) DENV compared to the wild-type (WT) DENV. Sequencing the structural proteins of HHA(res) resulted in two mutations, N67D and T155I, indicating a deletion of both N-glycosylation sites on the viral envelope E-glycoprotein. The HHA(res) DENV could replicate in mammalian and mosquito cells that are lacking dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) expression. In contrast, DC-SIGN expressing human cells namely monocyte-derived dendritic cells as well as DC-SIGN-transfected cells were no longer susceptible to HHA(res) DENV. This demonstrates a crucial role of the N-glycans in the E-glycoprotein in the infection of dendritic cells, which constitute primary target cells of DENV during viral pathogenesis in the human body.

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Authors+Show Affiliations

Alen MM
Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium. Marijke.Alen@rega.kuleuven.be
Dallmeier K
No affiliation info available
Balzarini J
No affiliation info available
Neyts J
No affiliation info available
Schols D
No affiliation info available

MeSH

AedesAgglutininsAnimalsAntiviral AgentsBinding SitesCell Adhesion MoleculesCell DifferentiationChlorocebus aethiopsDendritic CellsDengueDengue VirusDrug Resistance, ViralGenotypeGlycosylationHumansLectins, C-TypeLeukocytes, MononuclearLiliaceaeMutationPolysaccharidesReceptors, Cell SurfaceSequence Analysis, ProteinTransfectionVero CellsViral Envelope ProteinsVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23124109

Citation

Alen, Marijke M F., et al. "Crucial Role of the N-glycans On the Viral E-envelope Glycoprotein in DC-SIGN-mediated Dengue Virus Infection." Antiviral Research, vol. 96, no. 3, 2012, pp. 280-7.
Alen MM, Dallmeier K, Balzarini J, et al. Crucial role of the N-glycans on the viral E-envelope glycoprotein in DC-SIGN-mediated dengue virus infection. Antiviral Res. 2012;96(3):280-7.
Alen, M. M., Dallmeier, K., Balzarini, J., Neyts, J., & Schols, D. (2012). Crucial role of the N-glycans on the viral E-envelope glycoprotein in DC-SIGN-mediated dengue virus infection. Antiviral Research, 96(3), 280-7. https://doi.org/10.1016/j.antiviral.2012.10.007
Alen MM, et al. Crucial Role of the N-glycans On the Viral E-envelope Glycoprotein in DC-SIGN-mediated Dengue Virus Infection. Antiviral Res. 2012;96(3):280-7. PubMed PMID: 23124109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crucial role of the N-glycans on the viral E-envelope glycoprotein in DC-SIGN-mediated dengue virus infection. AU - Alen,Marijke M F, AU - Dallmeier,Kai, AU - Balzarini,Jan, AU - Neyts,Johan, AU - Schols,Dominique, Y1 - 2012/10/31/ PY - 2012/06/06/received PY - 2012/10/16/revised PY - 2012/10/21/accepted PY - 2012/11/6/entrez PY - 2012/11/6/pubmed PY - 2013/5/17/medline SP - 280 EP - 7 JF - Antiviral research JO - Antiviral Res VL - 96 IS - 3 N2 - We generated in the mosquito cell line C6/36 a dengue virus (DENV) resistant to Hippeastrum hybrid agglutinin (HHA), a carbohydrate-binding agent (CBA). The genotype and phenotype were characterized of the HHA resistant (HHA(res)) DENV compared to the wild-type (WT) DENV. Sequencing the structural proteins of HHA(res) resulted in two mutations, N67D and T155I, indicating a deletion of both N-glycosylation sites on the viral envelope E-glycoprotein. The HHA(res) DENV could replicate in mammalian and mosquito cells that are lacking dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) expression. In contrast, DC-SIGN expressing human cells namely monocyte-derived dendritic cells as well as DC-SIGN-transfected cells were no longer susceptible to HHA(res) DENV. This demonstrates a crucial role of the N-glycans in the E-glycoprotein in the infection of dendritic cells, which constitute primary target cells of DENV during viral pathogenesis in the human body. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/23124109/Crucial_role_of_the_N_glycans_on_the_viral_E_envelope_glycoprotein_in_DC_SIGN_mediated_dengue_virus_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(12)00246-X DB - PRIME DP - Unbound Medicine ER -