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Glutathione reductase from Oryza sativa increases acquired tolerance to abiotic stresses in a genetically modified Saccharomyces cerevisiae strain.
J Microbiol Biotechnol. 2012 Nov; 22(11):1557-67.JM

Abstract

Glutathione reductase (GR, E.C. 1.6.4.2) is an important enzyme that reduces glutathione disulfide (GSSG) to a sulfydryl form (GSH) in the presence of an NADPH-dependent system. This is a critical antioxidant mechanism. Owing to the significance of GR, this enzyme has been examined in a number of animals, plants, and microbes. We performed a study to evaluate the molecular properties of GR (OsGR) from rice (Oryza sativa). To determine whether heterologous expression of OsGR can reduce the deleterious effects of unfavorable abiotic conditions, we constructed a transgenic Saccharomyces cerevisiae strain expressing the GR gene cloned into the yeast expression vector p426GPD. OsGR expression was confirmed by a semiquantitative reverse transcriptase polymerase chain reaction (semiquantitative RT-PCR) assay, Western-blotting, and a test for enzyme activity. OsGR expression increased the ability of the yeast cells to adapt and recover from H2O2-induced oxidative stress and various stimuli including heat shock and exposure to menadione, heavy metals (iron, zinc, copper, and cadmium), sodium dodecyl sulfate (SDS), ethanol, and sulfuric acid. However, augmented OsGR expression did not affect the yeast fermentation capacity owing to reduction of OsGR by multiple factors produced during the fermentation process. These results suggest that ectopic OsGR expression conferred acquired tolerance by improving cellular homeostasis and resistance against different stresses in the genetically modified yeast strain, but did not affect fermentation ability.

Authors+Show Affiliations

Department of Biology, Kyungpook National University, Daegu 702-701, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23124348

Citation

Kim, Il-Sup, et al. "Glutathione Reductase From Oryza Sativa Increases Acquired Tolerance to Abiotic Stresses in a Genetically Modified Saccharomyces Cerevisiae Strain." Journal of Microbiology and Biotechnology, vol. 22, no. 11, 2012, pp. 1557-67.
Kim IS, Kim YS, Yoon HS. Glutathione reductase from Oryza sativa increases acquired tolerance to abiotic stresses in a genetically modified Saccharomyces cerevisiae strain. J Microbiol Biotechnol. 2012;22(11):1557-67.
Kim, I. S., Kim, Y. S., & Yoon, H. S. (2012). Glutathione reductase from Oryza sativa increases acquired tolerance to abiotic stresses in a genetically modified Saccharomyces cerevisiae strain. Journal of Microbiology and Biotechnology, 22(11), 1557-67.
Kim IS, Kim YS, Yoon HS. Glutathione Reductase From Oryza Sativa Increases Acquired Tolerance to Abiotic Stresses in a Genetically Modified Saccharomyces Cerevisiae Strain. J Microbiol Biotechnol. 2012;22(11):1557-67. PubMed PMID: 23124348.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione reductase from Oryza sativa increases acquired tolerance to abiotic stresses in a genetically modified Saccharomyces cerevisiae strain. AU - Kim,Il-Sup, AU - Kim,Young-Saeng, AU - Yoon,Ho-Sung, PY - 2012/11/6/entrez PY - 2012/11/6/pubmed PY - 2013/3/27/medline SP - 1557 EP - 67 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 22 IS - 11 N2 - Glutathione reductase (GR, E.C. 1.6.4.2) is an important enzyme that reduces glutathione disulfide (GSSG) to a sulfydryl form (GSH) in the presence of an NADPH-dependent system. This is a critical antioxidant mechanism. Owing to the significance of GR, this enzyme has been examined in a number of animals, plants, and microbes. We performed a study to evaluate the molecular properties of GR (OsGR) from rice (Oryza sativa). To determine whether heterologous expression of OsGR can reduce the deleterious effects of unfavorable abiotic conditions, we constructed a transgenic Saccharomyces cerevisiae strain expressing the GR gene cloned into the yeast expression vector p426GPD. OsGR expression was confirmed by a semiquantitative reverse transcriptase polymerase chain reaction (semiquantitative RT-PCR) assay, Western-blotting, and a test for enzyme activity. OsGR expression increased the ability of the yeast cells to adapt and recover from H2O2-induced oxidative stress and various stimuli including heat shock and exposure to menadione, heavy metals (iron, zinc, copper, and cadmium), sodium dodecyl sulfate (SDS), ethanol, and sulfuric acid. However, augmented OsGR expression did not affect the yeast fermentation capacity owing to reduction of OsGR by multiple factors produced during the fermentation process. These results suggest that ectopic OsGR expression conferred acquired tolerance by improving cellular homeostasis and resistance against different stresses in the genetically modified yeast strain, but did not affect fermentation ability. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/23124348/Glutathione_reductase_from_Oryza_sativa_increases_acquired_tolerance_to_abiotic_stresses_in_a_genetically_modified_Saccharomyces_cerevisiae_strain_ L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1202.02028 DB - PRIME DP - Unbound Medicine ER -