Tags

Type your tag names separated by a space and hit enter

Antioxidant and anti-inflammatory activities of Pittocaulon species from México.
Pharm Biol. 2013 Feb; 51(2):260-6.PB

Abstract

context: Stems and leaves of Pittocaulon spp. (Asteraceae) are used in Mexican traditional medicine as an anti-inflammatory substance and for the treatment of skin injuries.

OBJECTIVE

This study evaluated the antioxidant activity of methanol (MeOH) and dichloromethane (DC) extracts of five Pittocaulon species.

MATERIALS AND METHODS

DC and MeOH extracts from flowers, roots, and stems of Pittocaulon praecox (Cav.) H. Rob. & Brettell, P. bombycophole (Bullock) H. Rob. & Brettell, P. filare (Mc Vaugh) H. Rob. & Brettell, P. velatum (Greenm.) Rob. & Brettell and P. hintonii H. Rob. & Brettell.

RESULT AND DISCUSSION

In the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the flower extracts obtained with MeOH were the most active with IC(50) values ranging from 51.83 ± 4.08 to 154.19 ± 8.39 ppm. In the thiobarbituric acid reactive substances (TBARS) model, the best activity was shown by DC extracts of roots with IC(50) values ranging from 55.54 ± 1.28 to 160.82 ± 5.37 ppm. The MeOH extract of flowers of P. bombycophole had the highest IC(50) value in both DPPH (51.83 ± 4.08 ppm) and TBARS (39.78 ± 1.97 ppm). The samples with the best values in the antioxidant activity assays were evaluated in the anti-inflammatory tests. The DC root extract of P. velatum at a dose of 1 mg/ear produced the greatest reduction (84.96%) of the 2-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. This extract also reduced the activity of the enzyme myeloperoxidase (MPO) (73.65%) at the same dose. In contrast, DC root extract of this species did not show significant inhibition of the increase in paw edema induced by carrageenan at the doses tested (100 mg/kg).

CONCLUSION

These results support the traditional use of these plants as anti-inflammatory. DC extracts of P. velatum and MeOH extracts of P. bombycophole may be a potential resource of natural anti-inflammatory and antioxidant compounds, respectively. Additional studies must be done to identify the compounds responsible of the activity on these plants and to establish the mechanism of action.

Authors+Show Affiliations

Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Edificio 450, Oficina 107, Bogotá, Colombia.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23127192

Citation

Marín-Loaiza, J Camilo, et al. "Antioxidant and Anti-inflammatory Activities of Pittocaulon Species From México." Pharmaceutical Biology, vol. 51, no. 2, 2013, pp. 260-6.
Marín-Loaiza JC, Nieto-Camacho A, Céspedes CL. Antioxidant and anti-inflammatory activities of Pittocaulon species from México. Pharm Biol. 2013;51(2):260-6.
Marín-Loaiza, J. C., Nieto-Camacho, A., & Céspedes, C. L. (2013). Antioxidant and anti-inflammatory activities of Pittocaulon species from México. Pharmaceutical Biology, 51(2), 260-6. https://doi.org/10.3109/13880209.2012.718352
Marín-Loaiza JC, Nieto-Camacho A, Céspedes CL. Antioxidant and Anti-inflammatory Activities of Pittocaulon Species From México. Pharm Biol. 2013;51(2):260-6. PubMed PMID: 23127192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and anti-inflammatory activities of Pittocaulon species from México. AU - Marín-Loaiza,J Camilo, AU - Nieto-Camacho,Antonio, AU - Céspedes,Carlos L, Y1 - 2012/11/05/ PY - 2012/11/7/entrez PY - 2012/11/7/pubmed PY - 2013/6/29/medline SP - 260 EP - 6 JF - Pharmaceutical biology JO - Pharm Biol VL - 51 IS - 2 N2 - UNLABELLED: context: Stems and leaves of Pittocaulon spp. (Asteraceae) are used in Mexican traditional medicine as an anti-inflammatory substance and for the treatment of skin injuries. OBJECTIVE: This study evaluated the antioxidant activity of methanol (MeOH) and dichloromethane (DC) extracts of five Pittocaulon species. MATERIALS AND METHODS: DC and MeOH extracts from flowers, roots, and stems of Pittocaulon praecox (Cav.) H. Rob. & Brettell, P. bombycophole (Bullock) H. Rob. & Brettell, P. filare (Mc Vaugh) H. Rob. & Brettell, P. velatum (Greenm.) Rob. & Brettell and P. hintonii H. Rob. & Brettell. RESULT AND DISCUSSION: In the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the flower extracts obtained with MeOH were the most active with IC(50) values ranging from 51.83 ± 4.08 to 154.19 ± 8.39 ppm. In the thiobarbituric acid reactive substances (TBARS) model, the best activity was shown by DC extracts of roots with IC(50) values ranging from 55.54 ± 1.28 to 160.82 ± 5.37 ppm. The MeOH extract of flowers of P. bombycophole had the highest IC(50) value in both DPPH (51.83 ± 4.08 ppm) and TBARS (39.78 ± 1.97 ppm). The samples with the best values in the antioxidant activity assays were evaluated in the anti-inflammatory tests. The DC root extract of P. velatum at a dose of 1 mg/ear produced the greatest reduction (84.96%) of the 2-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. This extract also reduced the activity of the enzyme myeloperoxidase (MPO) (73.65%) at the same dose. In contrast, DC root extract of this species did not show significant inhibition of the increase in paw edema induced by carrageenan at the doses tested (100 mg/kg). CONCLUSION: These results support the traditional use of these plants as anti-inflammatory. DC extracts of P. velatum and MeOH extracts of P. bombycophole may be a potential resource of natural anti-inflammatory and antioxidant compounds, respectively. Additional studies must be done to identify the compounds responsible of the activity on these plants and to establish the mechanism of action. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/23127192/Antioxidant_and_anti_inflammatory_activities_of_Pittocaulon_species_from_México_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2012.718352 DB - PRIME DP - Unbound Medicine ER -