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Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation.
Immunopharmacol Immunotoxicol. 2013 Feb; 35(1):34-42.II

Abstract

The aerial parts of Artemisia capillaris (Compositae) have been used in traditional Korean medicine as a cholagogic, antipyretic, anti-inflammatory, and diuretic purposes. In our previous study, ethanolic extracts of the plant demonstrated a marked anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (J. Korean Soc. Appl. Biol. Chem., 2010, 53, 275-282). In the present study, capillarisin (CPS), a flavone, main constituent of A. capillaris, was examined for its anti-inflammatory activity in the cells. We found that CPS highly suppressed LPS-induced nitric oxide (NO) without exerting cytotoxic effects on RAW 264.7 cells. CPS inhibited the expression of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein and their mRNA in a dose-dependent manner. Also, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and prostaglandin E(2) (PGE(2)) secretion were decreased by CPS in LPS-stimulated macrophages. As a result, CPS inhibited proinflammatory cytokines, iNOS, and COX-2, which is attributed to the suppression of LPS-induced ERK, JNK, and nuclear factor-κB (NF-κB) activation. Therefore, we demonstrate here that CPS potentially inhibits the biomarkers related to inflammation through the abrogation of ERK, JNK, and NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases.

Authors+Show Affiliations

College of Oriental Medicine and Institute of Oriental Medicine, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23131135

Citation

Han, Suckbae, et al. "Capillarisin Inhibits iNOS, COX-2 Expression, and Proinflammatory Cytokines in LPS-induced RAW 264.7 Macrophages Via the Suppression of ERK, JNK, and NF-κB Activation." Immunopharmacology and Immunotoxicology, vol. 35, no. 1, 2013, pp. 34-42.
Han S, Lee JH, Kim C, et al. Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation. Immunopharmacol Immunotoxicol. 2013;35(1):34-42.
Han, S., Lee, J. H., Kim, C., Nam, D., Chung, W. S., Lee, S. G., Ahn, K. S., Cho, S. K., Cho, M., & Ahn, K. S. (2013). Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation. Immunopharmacology and Immunotoxicology, 35(1), 34-42. https://doi.org/10.3109/08923973.2012.736522
Han S, et al. Capillarisin Inhibits iNOS, COX-2 Expression, and Proinflammatory Cytokines in LPS-induced RAW 264.7 Macrophages Via the Suppression of ERK, JNK, and NF-κB Activation. Immunopharmacol Immunotoxicol. 2013;35(1):34-42. PubMed PMID: 23131135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation. AU - Han,Suckbae, AU - Lee,Jong Hyun, AU - Kim,Chulwon, AU - Nam,Dongwoo, AU - Chung,Won-Seok, AU - Lee,Seok-Geun, AU - Ahn,Kyoo Seok, AU - Cho,Somi K, AU - Cho,Moonjae, AU - Ahn,Kwang Seok, Y1 - 2012/11/07/ PY - 2012/11/8/entrez PY - 2012/11/8/pubmed PY - 2013/7/10/medline SP - 34 EP - 42 JF - Immunopharmacology and immunotoxicology JO - Immunopharmacol Immunotoxicol VL - 35 IS - 1 N2 - The aerial parts of Artemisia capillaris (Compositae) have been used in traditional Korean medicine as a cholagogic, antipyretic, anti-inflammatory, and diuretic purposes. In our previous study, ethanolic extracts of the plant demonstrated a marked anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (J. Korean Soc. Appl. Biol. Chem., 2010, 53, 275-282). In the present study, capillarisin (CPS), a flavone, main constituent of A. capillaris, was examined for its anti-inflammatory activity in the cells. We found that CPS highly suppressed LPS-induced nitric oxide (NO) without exerting cytotoxic effects on RAW 264.7 cells. CPS inhibited the expression of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein and their mRNA in a dose-dependent manner. Also, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and prostaglandin E(2) (PGE(2)) secretion were decreased by CPS in LPS-stimulated macrophages. As a result, CPS inhibited proinflammatory cytokines, iNOS, and COX-2, which is attributed to the suppression of LPS-induced ERK, JNK, and nuclear factor-κB (NF-κB) activation. Therefore, we demonstrate here that CPS potentially inhibits the biomarkers related to inflammation through the abrogation of ERK, JNK, and NF-κB p65 activation, and it may be a potential therapeutic candidate for the treatment of inflammatory diseases. SN - 1532-2513 UR - https://www.unboundmedicine.com/medline/citation/23131135/Capillarisin_inhibits_iNOS_COX_2_expression_and_proinflammatory_cytokines_in_LPS_induced_RAW_264_7_macrophages_via_the_suppression_of_ERK_JNK_and_NF_��B_activation_ L2 - https://www.tandfonline.com/doi/full/10.3109/08923973.2012.736522 DB - PRIME DP - Unbound Medicine ER -