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Immunogenicity of monococcum wheat in celiac patients.
Am J Clin Nutr. 2012 Dec; 96(6):1339-45.AJ

Abstract

BACKGROUND

Research is intense to find wheat of low or null toxicity for patients with celiac disease (CD). Among candidates, there are diploid wheat species.

OBJECTIVE

We compared the immunological properties of 2 lines of diploid monococcum wheat (Triticum monococcum ssp. monococcum), Monlis and ID331, with those of common wheat (Triticum aestivum).

DESIGN

Interferon-γ production and the proliferation of intestinal gliadin-specific T cell lines and clones were measured as evidence of T cell activation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines. Furthermore, organ cultures of jejunal biopsies from 28 CD patients were set up to assess the effects of PT gliadin on innate and adaptive immune response by using immunohistochemistry.

RESULTS

Monlis and ID331 induced interferon-γ production and proliferation in celiac mucosal T cells. In organ cultures, Monlis PT digest induced a significant increase of IL-15 epithelial expression and crypt enterocyte proliferation, whereas ID331 had no effect. Both monococcum lines caused intraepithelial T cell infiltration and lamina propria T cell activation.

CONCLUSIONS

Our data show that the monococcum lines Monlis and ID331 activate the CD T cell response and suggest that these lines are toxic for celiac patients. However, ID331 is likely to be less effective in inducing CD because of its inability to activate the innate immune pathways.

Authors+Show Affiliations

Institute of Food Sciences, Immunobiology, National Council Research, Avellino, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23134879

Citation

Gianfrani, Carmen, et al. "Immunogenicity of Monococcum Wheat in Celiac Patients." The American Journal of Clinical Nutrition, vol. 96, no. 6, 2012, pp. 1339-45.
Gianfrani C, Maglio M, Rotondi Aufiero V, et al. Immunogenicity of monococcum wheat in celiac patients. Am J Clin Nutr. 2012;96(6):1339-45.
Gianfrani, C., Maglio, M., Rotondi Aufiero, V., Camarca, A., Vocca, I., Iaquinto, G., Giardullo, N., Pogna, N., Troncone, R., Auricchio, S., & Mazzarella, G. (2012). Immunogenicity of monococcum wheat in celiac patients. The American Journal of Clinical Nutrition, 96(6), 1339-45. https://doi.org/10.3945/ajcn.112.040485
Gianfrani C, et al. Immunogenicity of Monococcum Wheat in Celiac Patients. Am J Clin Nutr. 2012;96(6):1339-45. PubMed PMID: 23134879.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunogenicity of monococcum wheat in celiac patients. AU - Gianfrani,Carmen, AU - Maglio,Mariatonia, AU - Rotondi Aufiero,Vera, AU - Camarca,Alessandra, AU - Vocca,Immacolata, AU - Iaquinto,Gaetano, AU - Giardullo,Nicola, AU - Pogna,Norberto, AU - Troncone,Riccardo, AU - Auricchio,Salvatore, AU - Mazzarella,Giuseppe, Y1 - 2012/11/07/ PY - 2012/11/9/entrez PY - 2012/11/9/pubmed PY - 2013/1/26/medline SP - 1339 EP - 45 JF - The American journal of clinical nutrition JO - Am J Clin Nutr VL - 96 IS - 6 N2 - BACKGROUND: Research is intense to find wheat of low or null toxicity for patients with celiac disease (CD). Among candidates, there are diploid wheat species. OBJECTIVE: We compared the immunological properties of 2 lines of diploid monococcum wheat (Triticum monococcum ssp. monococcum), Monlis and ID331, with those of common wheat (Triticum aestivum). DESIGN: Interferon-γ production and the proliferation of intestinal gliadin-specific T cell lines and clones were measured as evidence of T cell activation by peptic and tryptic (PT) digests of gliadins from 2 monococcum lines. Furthermore, organ cultures of jejunal biopsies from 28 CD patients were set up to assess the effects of PT gliadin on innate and adaptive immune response by using immunohistochemistry. RESULTS: Monlis and ID331 induced interferon-γ production and proliferation in celiac mucosal T cells. In organ cultures, Monlis PT digest induced a significant increase of IL-15 epithelial expression and crypt enterocyte proliferation, whereas ID331 had no effect. Both monococcum lines caused intraepithelial T cell infiltration and lamina propria T cell activation. CONCLUSIONS: Our data show that the monococcum lines Monlis and ID331 activate the CD T cell response and suggest that these lines are toxic for celiac patients. However, ID331 is likely to be less effective in inducing CD because of its inability to activate the innate immune pathways. SN - 1938-3207 UR - https://www.unboundmedicine.com/medline/citation/23134879/Immunogenicity_of_monococcum_wheat_in_celiac_patients_ L2 - https://academic.oup.com/ajcn/article-lookup/doi/10.3945/ajcn.112.040485 DB - PRIME DP - Unbound Medicine ER -