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Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures.

Abstract

BACKGROUND

Propolis and honey are natural bee products with wide range of biological and medicinal properties. The study investigated antimicrobial activity of ethyl alcohol extraction of propolis collected from Saudi Arabia (EEPS) and from Egypt (EEPE), and their synergistic effect when used with honey. Single and polymicrobial cultures of antibiotic resistant human pathogens were tested.

MATERIAL AND METHODS

Staphylococcus aureus (S. aureus),), Escherichia coli (E. coli) and Candida albicans (C.albicans) were cultured in 10-100% (v/v) honey diluted in broth, or 0.08-1.0% (weight/volume) EEPS and EEPE diluted in broth. Four types of polymicrobial cultures were prepared by culturing the isolates with each other in broth (control) and broth containing various concentrations of honey or propolis. Microbial growth was assessed on solid plate media after 24 h incubation.

RESULTS

EEPS and EEPE inhibited antibiotic resistant E.coli, and S.aureus, and C.albicans in single and polymicrobial cultures. S.aureus became more susceptible when it was cultured with E.coli or C.albicans or when all cultured together. C.albicans became more susceptible when it was cultured with S.aureus or with E.coli and S. aureus together. The presence of ethyl alcohol or honey potentiated antimicrobial effect of propolis toward entire microbes tested in single or polymicrobial cultures. EEPS had lower MIC toward E.coli and C.albicans than EEPE. When propolis was mixed with honey, EEPS showed lower MIC than EEPE. In addition, honey showed lower MIC toward entire microbes when mixed with EEPS than when it was mixed with EEPE.

CONCLUSION

1) propolis prevents the growth of the microorganisms in single and mixed microbial cultures, and has synergistic effect when used with honey or ethyl alcohol, 2) the antimicrobial property of propolis varies with geographical origin, and 3) this study will pave the way to isolate active ingredients from honey and propolis to be further tested individually or in combination against human resistant infections.

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  • Authors+Show Affiliations

    ,

    Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University-Riyadh, Kingdom of Saudi Arabia. Drnoori6@yahoo.com

    , , ,

    Source

    MeSH

    Anti-Bacterial Agents
    Antifungal Agents
    Candida albicans
    Drug Resistance, Multiple, Bacterial
    Drug Resistance, Multiple, Fungal
    Drug Synergism
    Egypt
    Escherichia coli
    Ethanol
    Honey
    Humans
    Microbial Sensitivity Tests
    Propolis
    Saudi Arabia
    Staphylococcus aureus

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    23136543

    Citation

    Al-Waili, Noori, et al. "Synergistic Effects of Honey and Propolis Toward Drug Multi-resistant Staphylococcus Aureus, Escherichia Coli and Candida Albicans Isolates in Single and Polymicrobial Cultures." International Journal of Medical Sciences, vol. 9, no. 9, 2012, pp. 793-800.
    Al-Waili N, Al-Ghamdi A, Ansari MJ, et al. Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. Int J Med Sci. 2012;9(9):793-800.
    Al-Waili, N., Al-Ghamdi, A., Ansari, M. J., Al-Attal, Y., & Salom, K. (2012). Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. International Journal of Medical Sciences, 9(9), pp. 793-800. doi:10.7150/ijms.4722.
    Al-Waili N, et al. Synergistic Effects of Honey and Propolis Toward Drug Multi-resistant Staphylococcus Aureus, Escherichia Coli and Candida Albicans Isolates in Single and Polymicrobial Cultures. Int J Med Sci. 2012;9(9):793-800. PubMed PMID: 23136543.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Synergistic effects of honey and propolis toward drug multi-resistant Staphylococcus aureus, Escherichia coli and Candida albicans isolates in single and polymicrobial cultures. AU - Al-Waili,Noori, AU - Al-Ghamdi,Ahmad, AU - Ansari,Mohammad Javed, AU - Al-Attal,Y, AU - Salom,Khelod, Y1 - 2012/10/26/ PY - 2012/06/10/received PY - 2012/08/19/accepted PY - 2012/11/9/entrez PY - 2012/11/9/pubmed PY - 2013/4/23/medline KW - Fungi KW - Honey KW - Propolis KW - bacteria KW - synergism. SP - 793 EP - 800 JF - International journal of medical sciences JO - Int J Med Sci VL - 9 IS - 9 N2 - BACKGROUND: Propolis and honey are natural bee products with wide range of biological and medicinal properties. The study investigated antimicrobial activity of ethyl alcohol extraction of propolis collected from Saudi Arabia (EEPS) and from Egypt (EEPE), and their synergistic effect when used with honey. Single and polymicrobial cultures of antibiotic resistant human pathogens were tested. MATERIAL AND METHODS: Staphylococcus aureus (S. aureus),), Escherichia coli (E. coli) and Candida albicans (C.albicans) were cultured in 10-100% (v/v) honey diluted in broth, or 0.08-1.0% (weight/volume) EEPS and EEPE diluted in broth. Four types of polymicrobial cultures were prepared by culturing the isolates with each other in broth (control) and broth containing various concentrations of honey or propolis. Microbial growth was assessed on solid plate media after 24 h incubation. RESULTS: EEPS and EEPE inhibited antibiotic resistant E.coli, and S.aureus, and C.albicans in single and polymicrobial cultures. S.aureus became more susceptible when it was cultured with E.coli or C.albicans or when all cultured together. C.albicans became more susceptible when it was cultured with S.aureus or with E.coli and S. aureus together. The presence of ethyl alcohol or honey potentiated antimicrobial effect of propolis toward entire microbes tested in single or polymicrobial cultures. EEPS had lower MIC toward E.coli and C.albicans than EEPE. When propolis was mixed with honey, EEPS showed lower MIC than EEPE. In addition, honey showed lower MIC toward entire microbes when mixed with EEPS than when it was mixed with EEPE. CONCLUSION: 1) propolis prevents the growth of the microorganisms in single and mixed microbial cultures, and has synergistic effect when used with honey or ethyl alcohol, 2) the antimicrobial property of propolis varies with geographical origin, and 3) this study will pave the way to isolate active ingredients from honey and propolis to be further tested individually or in combination against human resistant infections. SN - 1449-1907 UR - https://www.unboundmedicine.com/medline/citation/23136543/Synergistic_effects_of_honey_and_propolis_toward_drug_multi_resistant_Staphylococcus_aureus_Escherichia_coli_and_Candida_albicans_isolates_in_single_and_polymicrobial_cultures_ L2 - http://www.medsci.org/v09p0793.htm DB - PRIME DP - Unbound Medicine ER -