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Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis.
Microbiology (Reading). 2013 Jan; 159(Pt 1):68-76.M

Abstract

The role of the CcpC regulatory protein as a repressor of the genes encoding the tricarboxylic acid branch enzymes of the Krebs cycle (citrate synthase, citZ; aconitase, citB; and isocitrate dehydrogenase, citC) has been established for both Bacillus subtilis and Listeria monocytogenes. In addition, hyperexpression of citB-lacZ reporter constructs in an aconitase null mutant strain has been reported for B. subtilis. We show here that such hyperexpression of citB occurs in L. monocytogenes as well as in B. subtilis and that in both species the hyperexpression is unexpectedly dependent on CcpC. We propose a revision of the existing CcpC-citB regulatory scheme and suggest a mechanism of regulation in which CcpC represses citB expression at low citrate levels and activates citB expression when citrate levels are high.

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Authors+Show Affiliations

Mittal M
Program in Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA.
Pechter KB
Program in Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA.
Picossi S
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Kim HJ
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Kerstein KO
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA.
Sonenshein AL
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA 02111, USA. Program in Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA.

MeSH

Aconitate HydrataseArtificial Gene FusionBacillus subtilisGene DeletionGene Expression Regulation, BacterialGenes, ReporterListeria monocytogenesRepressor Proteinsbeta-Galactosidase

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23139400

Citation

Mittal, Meghna, et al. "Dual Role of CcpC Protein in Regulation of Aconitase Gene Expression in Listeria Monocytogenes and Bacillus Subtilis." Microbiology (Reading, England), vol. 159, no. Pt 1, 2013, pp. 68-76.
Mittal M, Pechter KB, Picossi S, et al. Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis. Microbiology (Reading). 2013;159(Pt 1):68-76.
Mittal, M., Pechter, K. B., Picossi, S., Kim, H. J., Kerstein, K. O., & Sonenshein, A. L. (2013). Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis. Microbiology (Reading, England), 159(Pt 1), 68-76. https://doi.org/10.1099/mic.0.063388-0
Mittal M, et al. Dual Role of CcpC Protein in Regulation of Aconitase Gene Expression in Listeria Monocytogenes and Bacillus Subtilis. Microbiology (Reading). 2013;159(Pt 1):68-76. PubMed PMID: 23139400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual role of CcpC protein in regulation of aconitase gene expression in Listeria monocytogenes and Bacillus subtilis. AU - Mittal,Meghna, AU - Pechter,Kieran B, AU - Picossi,Silvia, AU - Kim,Hyun-Jin, AU - Kerstein,Kathryn O, AU - Sonenshein,Abraham L, Y1 - 2012/11/08/ PY - 2012/11/10/entrez PY - 2012/11/10/pubmed PY - 2013/5/31/medline SP - 68 EP - 76 JF - Microbiology (Reading, England) JO - Microbiology (Reading) VL - 159 IS - Pt 1 N2 - The role of the CcpC regulatory protein as a repressor of the genes encoding the tricarboxylic acid branch enzymes of the Krebs cycle (citrate synthase, citZ; aconitase, citB; and isocitrate dehydrogenase, citC) has been established for both Bacillus subtilis and Listeria monocytogenes. In addition, hyperexpression of citB-lacZ reporter constructs in an aconitase null mutant strain has been reported for B. subtilis. We show here that such hyperexpression of citB occurs in L. monocytogenes as well as in B. subtilis and that in both species the hyperexpression is unexpectedly dependent on CcpC. We propose a revision of the existing CcpC-citB regulatory scheme and suggest a mechanism of regulation in which CcpC represses citB expression at low citrate levels and activates citB expression when citrate levels are high. SN - 1465-2080 UR - https://www.unboundmedicine.com/medline/citation/23139400/Dual_role_of_CcpC_protein_in_regulation_of_aconitase_gene_expression_in_Listeria_monocytogenes_and_Bacillus_subtilis_ L2 - http://mic.microbiologyresearch.org/pubmed/content/journal/micro/10.1099/mic.0.063388-0 DB - PRIME DP - Unbound Medicine ER -